Ppp2r2d (NM_026391) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207239
Ppp2r2d (Myc-DDK-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppp2r2d
Synonyms 1300017E19Rik; D7Ertd753e; MDS026
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207239 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGAGCTGGAGGCGGCGGCTGCCCGGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTGA
AGGGCGCTGTCGACGAGGACGTGGCGGAAGCCGACATCATCTCCACCGTTGAGTTTAACTACTCTGGAGA
CCTTCTTGCAACAGGAGACAAAGGTGGCAGAGTTGTTATTTTCCAGCGGGAACAAGAGAATAAAGGCCGC
GCTCACTCTAGGGGAGAGTACAATGTTTACAGTACCTTTCAGAGTCATGAGCCAGAGTTTGACTATTTGA
AAAGTCTAGAAATTGAAGAAAAAATTAATAAAATCAGGTGGTTACCGCAACAGAATGCTGCTCATTTTCT
ACTCTCTACAAATGATAAAACTATTAAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTAT
AACTTGAAAGATGAAGATGGACGACTTCGAGACCCATTTAGAATTACGGCACTACGGGTTCCAATATTGA
AGCCCATGGACCTTATGGTAGAAGCAAGTCCACGACGAATTTTTGCAAATGCTCATACATATCACATAAA
TTCCATTTCAGTAAATAGTGATCATGAAACATATCTCTCTGCAGATGATCTGAGAATTAACCTATGGCAT
TTAGAAATCACAGATAGAAGCTTCAACATTGTGGACATCAAGCCAGCTAATATGGAGGAGCTGACAGAAG
TCATCACTGCCGCAGAGTTCCACCCACATCAGTGCAATGTATTTGTTTACAGCAGCAGCAAGGGCACCAT
CAGGCTGTGTGACATGCGTTCCTCTGCCCTATGTGACAGGCATGCCAAGTTTTTTGAAGAGCCAGAAGAT
CCCAGCAGTAGATCCTTCTTCTCAGAAATAATCTCATCTATATCTGATGTCAAGTTCAGCCACAGTGGTC
GATACATGATGACCAGAGACTATCTGTCGGTGAAGGTCTGGGACCTCAACATGGAGGGCAGGCCTGTGGA
GACCCACCAGGTACATGAGTACCTGCGAAGCAAGCTCTGCTCCTTGTATGAGAACGACTGCATCTTTGAC
AAGTTCGAGTGCTGCTGGAACGGTTCAGACAGTGCCATTATGACGGGGTCCTACAACAACTTCTTTAGAA
TGTTTGATAGAAACACTCGGAGGGATGTTACACTGGAAGCCTCAAGAGAGAACAGCAAACCCCGAGCCAG
CCTGAAGCCCCGGAAAGTATGTACAGGGGGTAAGAGAAAGAAAGACGAGATTAGCGTGGACAGTTTGGAC
TTCAATAAGAAGATCCTCCACACAGCCTGGCACCCCATGGAGAGCATTATTGCTGTAGCTGCCACCAATA
ACTTGTATATATTCCAGGACAAAATTAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207239 protein sequence
Red=Cloning site Green=Tags(s)

MAGAGGGGCPAGGNDFQWCFSQVKGAVDEDVAEADIISTVEFNYSGDLLATGDKGGRVVIFQREQENKGR
AHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAHFLLSTNDKTIKLWKISERDKRAEGY
NLKDEDGRLRDPFRITALRVPILKPMDLMVEASPRRIFANAHTYHINSISVNSDHETYLSADDLRINLWH
LEITDRSFNIVDIKPANMEELTEVITAAEFHPHQCNVFVYSSSKGTIRLCDMRSSALCDRHAKFFEEPED
PSSRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKVWDLNMEGRPVETHQVHEYLRSKLCSLYENDCIFD
KFECCWNGSDSAIMTGSYNNFFRMFDRNTRRDVTLEASRENSKPRASLKPRKVCTGGKRKKDEISVDSLD
FNKKILHTAWHPMESIIAVAATNNLYIFQDKIN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026391
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026391.2, NP_080667.1
RefSeq Size 2079 bp
RefSeq ORF 1362 bp
Locus ID 52432
UniProt ID Q925E7
Cytogenetics 7 82.95 cM
MW 52 kDa
Summary B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026391" in other vectors (3)
SKU Description Size Price
MG207239 Ppp2r2d (tGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d) 10 ug
$657.00
MR207239L3 Lenti ORF clone of Ppp2r2d (Myc-DDK-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d) 10 ug
$757.00
MR207239L4 Lenti ORF clone of Ppp2r2d (mGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d) 10 ug
$757.00

Plasmid Map for MR207239

Download sequence for

Cloning scheme for MR207239

Circular map for MR207239

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