Casp2 (NM_007610) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR207227
Casp2 (Myc-DDK-tagged) - Mouse caspase 2 (Casp2)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Casp2
Synonyms Casp; CASP-2; Ich-; ICH-1; Nedd; NEDD-2; Nedd2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207227 representing NM_007610
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCCGAGCGGGAGGTCGCAGTCCTCCCTGCACAGGAAGGGGCTGATGGCGGCTGACAGGAGGA
GCAGGATTTTGGCAGTGTGTGGAATGCATCCTGACCACCAGGAAACACTGAAAAAGAATCGAGTGGTGCT
GGCCAAGCAGCTGCTGCTGAGCGAGCTGTTAGAACACCTCCTAGAGAAGGACATTATCACTTTGGAAATG
AGGGAGCTCATCCAGGCCAAAGGGGGCAGTTTCAGCCAGAATGTGGAACTCCTCAACCTGCTGCCAAAGA
GAGGACCCCAGGCTTTTGATGCCTTCTGTGAAGCCCTGCGGGAGACCAGGCAGGGTCACTTGGAAGACTT
ACTGCTCACAACCCTCTCAGATATTCAGCACGTACTCCCACCGTTGAGCTGTGACTATGACACAAGTCTC
CCTTTCTCGGTGTGTGAGTCCTGCCCTCCTCACAAGCAGCTCCGCCTATCCACAGATGCTACGGAACACT
CCTTAGATAATGGTGATGGTCCTCCCTGTCTTCTGGTGAAGCCATGCACTCCTGAGTTTTACCAGGCACA
CTACCAGCTGGCCTATAGGTTGCAATCTCAGCCCCGTGGCTTGGCACTGGTGCTGAGCAATGTGCACTTC
ACTGGAGAGAAAGACCTGGAATTCCGCTCTGGAGGGGATGTGGACCACACTACTCTAGTCACCCTCTTCA
AGCTTTTGGGCTACAATGTCCATGTGCTACATGACCAGACCGCACAGGAAATGCAAGAGAAACTTCAGAA
TTTTGCACAGTTACCTGCACACCGGGTCACAGACTCCTGCGTAGTGGCACTCCTCTCACATGGTGTGGAA
GGTGGCATCTATGGTGTAGATGGCAAACTGCTTCAGCTCCAAGAGGTTTTTCGACTTTTTGACAATGCTA
ACTGTCCAAGTCTACAGAACAAGCCAAAAATGTTCTTCATCCAAGCATGTCGTGGAGATGAGACAGATAG
AGGTGTCGACCAGCAAGATGGAAAGAACCACACACAATCCCCTGGATGTGAGGAGAGTGATGCTGGCAAA
GAGGAGTTGATGAAGATGAGACTGCCTACTCGCTCAGACATGATATGTGGCTATGCTTGCCTTAAAGGTA
ATGCTGCCATGCGGAACACCAAACGGGGTTCCTGGTACATTGAGGCCCTCACTCAGGTGTTCTCTGAAAG
AGCTTGTGACATGCACGTGGCCGACATGCTTGTTAAGGTGAATGCCCTTATCAAGGAGCGTGAAGGCTAT
GCCCCTGGCACAGAATTCCACCGATGCAAGGAGATGTCTGAGTACTGTAGTACTCTGTGCCAGCAACTCT
ACCTGTTCCCAGGCTACCCACCCACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR207227 representing NM_007610
Red=Cloning site Green=Tags(s)

MAAPSGRSQSSLHRKGLMAADRRSRILAVCGMHPDHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEM
RELIQAKGGSFSQNVELLNLLPKRGPQAFDAFCEALRETRQGHLEDLLLTTLSDIQHVLPPLSCDYDTSL
PFSVCESCPPHKQLRLSTDATEHSLDNGDGPPCLLVKPCTPEFYQAHYQLAYRLQSQPRGLALVLSNVHF
TGEKDLEFRSGGDVDHTTLVTLFKLLGYNVHVLHDQTAQEMQEKLQNFAQLPAHRVTDSCVVALLSHGVE
GGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHTQSPGCEESDAGK
EELMKMRLPTRSDMICGYACLKGNAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGY
APGTEFHRCKEMSEYCSTLCQQLYLFPGYPPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007610
ORF Size 1356 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007610.2
RefSeq Size 3463 bp
RefSeq ORF 1359 bp
Locus ID 12366
UniProt ID P29594
Cytogenetics 6 20.54 cM
MW 51.1 kDa
Summary This gene encodes the evolutionarily ancient and most conserved member of the cysteine proteases that plays important role in stress-induced apoptosis, DNA repair and tumor suppression. Mice lacking the encoded protein develop normally but display cell type-specific apoptotic defects. Germ cells and oocytes from such mice were found to be resistant to cell death after treatment with chemotherapeutic drugs. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_007610" in other vectors (4)
SKU Description Size Price
MC206011 Casp2 (untagged) - Mouse caspase 2 (Casp2), (10ug) 10 ug
$686.00
MG207227 Casp2 (tGFP-tagged) - Mouse caspase 2 (Casp2) 10 ug
$886.00
MR207227L3 Lenti ORF clone of Casp2 (Myc-DDK-tagged) - Mouse caspase 2 (Casp2) 10 ug
$986.00
MR207227L4 Lenti ORF clone of Casp2 (mGFP-tagged) - Mouse caspase 2 (Casp2) 10 ug
$986.00

Plasmid Map for MR207227

Download sequence for

Cloning scheme for MR207227

Circular map for MR207227

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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