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Ppp2r5c (NM_001081458) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207212
Ppp2r5c (Myc-DDK-tagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), gamma isoform (Ppp2r5c), transcript variant 3
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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Other products for "Ppp2r5c or NM_001081458"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppp2r5c
Synonyms 2610043M05Rik; 2700063L20Rik; AI060890; AW545884; C85228; D12Bwg0916e; mKIAA0044
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207212 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGACATGTAATAAAGCGGGCAGCGGGATGGTGGTGGATGCGGCCAGCTCCAACGGGCCTTTCCAGC
CCGTGGCCCTTCTCCACATTCGAGATGTTCCTCCTGCGGATCAAGAGAAGCTTTTTATCCAGAAGCTACG
CCAGTGTTGTGTCCTCTTTGACTTTGTCTCTGACCCACTGAGTGACCTGAAGTGGAAGGAAGTAAAGCGC
GCTGCGCTGAGCGAGATGGTGGAGTATATCACCCACAACCGGAACGTGATCACGGAGCCCATTTACCCCG
AGGCCGTCCACATGTTTGCAGTTAACATGTTCCGAACCTTGCCACCTTCCTCCAATCCCACGGGAGCAGA
ATTCGACCCAGAAGAGGATGAACCAACGTTAGAAGCAGCCTGGCCTCATCTGCAGCTTGTTTATGAATTT
TTCTTAAGATTTTTAGAGTCTCCAGATTTCCAACCCAATATAGCAAAGAAATATATTGATCAGAAGTTTG
TATTGCAGCTTCTAGAGCTGTTTGACAGCGAGGATCCTCGGGAGAGAGATTTTCTAAAAACCACCCTGCA
CAGAATCTATGGGAAGTTCTTAGGCCTGCGTGCTTACATCAGGAAACAGATCAATAATATATTTTATAGG
TTTATCTATGAGACAGAGCATCACAATGGCATAGCGGAGTTACTGGAGATCCTGGGAAGTATAATTAATG
GATTTGCCTTACCACTGAAGGAGGAACACAAGATTTTCCTGCTGAAGGTGTTGCTGCCCTTGCACAAAGT
GAAGTCCCTGAGTGTCTACCATCCCCAGCTGGCGTACTGTGTCGTGCAGTTTTTAGAGAAGGACAGCACC
CTCACTGAACCAGTGGTAATGGCACTTCTCAAATACTGGCCAAAGACTCACAGTCCAAAAGAAGTAATGT
TCTTAAATGAATTAGAAGAAATTTTAGATGTAATTGAACCATCAGAGTTTGTGAAGATCATGGAGCCTCT
TTTCCGACAGTTAGCCAAATGTGTTTCCAGCCCTCACTTCCAGGTGGCCGAGCGGGCGCTCTATTACTGG
AACAACGAGTACATCATGAGTTTAATCAGTGACAACGCAGCGAAGATTCTGCCCATCATGTTTCCGTCCT
TATACCGCAACTCAAAGACCCACTGGAACAAGACAATACACGGCTTGATATACAACGCCCTGAAACTCTT
CATGGAGATGAACCAAAAACTCTTCGATGACTGCACTCAGCAGTTCAAAGCAGAGAAACTCAAAGAGAAG
CTAAAAATGAAAGAGCGAGAAGAAGCATGGGTTAAAATAGAAAATCTAGCCAAAGCGAATCCCCAGGTAC
TAAAAAAGAGAGTAACTCGGGAGTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207212 protein sequence
Red=Cloning site Green=Tags(s)

MLTCNKAGSGMVVDAASSNGPFQPVALLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKR
AALSEMVEYITHNRNVITEPIYPEAVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEF
FLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYR
FIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDST
LTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYW
NNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEK
LKMKEREEAWVKIENLAKANPQVLKKRVTREC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081458
ORF Size 1356 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081458.3
RefSeq Size 2383 bp
RefSeq ORF 1359 bp
Locus ID 26931
UniProt ID Q60996
Cytogenetics 12 60.56 cM
MW 52.8 kDa
Summary The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001081458" in other vectors (4)
SKU Description Size Price
MC215903 Ppp2r5c (untagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), gamma isoform (Ppp2r5c), transcript variant 3, (10ug) 10 ug
$503.00
MG207212 Ppp2r5c (tGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), gamma isoform (Ppp2r5c), transcript variant 3 10 ug
$657.00
MR207212L3 Lenti ORF clone of Ppp2r5c (Myc-DDK-tagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), gamma isoform (Ppp2r5c), transcript variant 3 10 ug
$757.00
MR207212L4 Lenti ORF clone of Ppp2r5c (mGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), gamma isoform (Ppp2r5c), transcript variant 3 10 ug
$757.00

Plasmid Map for MR207212

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207212
Circular map for MR207212

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