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Tph1 (NM_009414) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207141
Tph1 (Myc-DDK-tagged) - Mouse tryptophan hydroxylase 1 (Tph1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tph1 or NM_009414"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tph1
Synonyms Tph
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207141 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTGAAGACAACAAGGAGAACAAAGAGAACAAAGACCATTCCTCCGAAAGAGGGAGAGTGACTCTCA
TCTTCTCCTTGGAGAATGAAGTCGGAGGACTCATAAAAGTGCTGAAAATCTTCCAGGAGAATCATGTGAG
CCTGTTACACATCGAGTCCCGGAAATCAAAGCAAAGAAATTCAGAATTTGAGATATTTGTTGACTGCGAC
ATCAGCCGAGAACAGTTGAATGACATCTTCCCCCTGCTGAAGTCGCACGCCACCGTCCTCTCGGTGGACT
CGCCCGATCAGCTCACTGCGAAGGAAGACGTTATGGAGACTGTCCCTTGGTTTCCAAAGAAGATTTCTGA
CCTGGACTTCTGCGCCAACAGAGTGCTGTTGTATGGATCCGAACTTGACGCCGACCACCCTGGCTTCAAA
GACAATGTCTATCGTAGAAGACGAAAGTATTTTGCAGAGTTGGCTATGAACTACAAACATGGGGACCCCA
TTCCCAAGATTGAATTCACGGAAGAAGAGATTAAGACCTGGGGGACCATCTTCCGAGAGCTAAACAAACT
CTACCCGACCCACGCCTGCAGGGAGTACCTCAGAAACCTCCCTTTGCTCTCAAAATACTGTGGCTATCGG
GAAGACAACATCCCGCAACTGGAGGATGTCTCCAACTTTTTAAAAGAACGCACTGGGTTTTCCATCCGTC
CTGTGGCTGGTTACCTCTCACCGAGAGATTTTCTGTCGGGGTTAGCCTTTCGAGTCTTTCACTGCACTCA
GTATGTGAGACACAGTTCAGATCCCCTCTACACTCCAGAGCCAGACACCTGCCATGAACTCCTAGGCCAC
GTTCCTCTCTTGGCTGAACCCAGTTTTGCTCAATTCTCCCAAGAAATTGGCCTGGCTTCCCTTGGAGCTT
CAGAGGAGACAGTTCAAAAACTGGCAACGTGCTACTTTTTCACTGTGGAGTTTGGGCTGTGCAAACAAGA
TGGACAGCTGAGAGTCTTTGGGGCCGGCTTGCTTTCTTCCATCAGTGAACTCAAACATGCACTTTCTGGA
CATGCCAAAGTCAAGCCCTTTGATCCCAAGATTGCCTGTAAACAGGAATGTCTCATCACGAGCTTCCAGG
ATGTCTACTTTGTATCTGAGAGCTTTGAAGATGCAAAGGAGAAGATGAGAGAATTTGCCAAGACCGTGAA
GCGCCCGTTTGGACTGAAGTACAACCCGTACACACAGAGTGTTCAGGTTCTCAGAGACACCAAGAGCATA
ACTAGTGCCATGAATGAGTTGCGGTATGACCTTGATGTCATCAGTGATGCCCTCGCTAGGGTCACCAGGT
GGCCCAGTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207141 protein sequence
Red=Cloning site Green=Tags(s)

MIEDNKENKENKDHSSERGRVTLIFSLENEVGGLIKVLKIFQENHVSLLHIESRKSKQRNSEFEIFVDCD
ISREQLNDIFPLLKSHATVLSVDSPDQLTAKEDVMETVPWFPKKISDLDFCANRVLLYGSELDADHPGFK
DNVYRRRRKYFAELAMNYKHGDPIPKIEFTEEEIKTWGTIFRELNKLYPTHACREYLRNLPLLSKYCGYR
EDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPLYTPEPDTCHELLGH
VPLLAEPSFAQFSQEIGLASLGASEETVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSG
HAKVKPFDPKIACKQECLITSFQDVYFVSESFEDAKEKMREFAKTVKRPFGLKYNPYTQSVQVLRDTKSI
TSAMNELRYDLDVISDALARVTRWPSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009414
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009414.3
RefSeq Size 4581 bp
RefSeq ORF 1344 bp
Locus ID 21990
UniProt ID P17532
Cytogenetics 7 30.43 cM
MW 51.3 kDa
Summary This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase family. The encoded protein is one of two tryptophan hydroxylase enzymes that catalyze the first and rate limiting step in the biosynthesis of the hormone and neurotransmitter, serotonin. This gene is expressed in peripheral organs, while tryptophan hydroxylase 2 is expressed in neurons. The encoded protein is involved in the development of hypoxia-induced elevations in pulmonary pressures and pulmonary vascular remodeling, and has also been implicated as a regulator of immune tolerance. Disruption of this gene is associated with cardiac dysfunction. Alternative splicing results in multiple transcript variants. provided by RefSeq, Feb 2013
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"NM_009414" in other vectors (4)
SKU Description Size Price
MC202539 Tph1 (untagged) - Mouse tryptophan hydroxylase 1 (Tph1), transcript variant 1, (10ug) 10 ug
$457.00
MG207141 Tph1 (tGFP-tagged) - Mouse tryptophan hydroxylase 1 (Tph1) 10 ug
$657.00
MR207141L3 Lenti ORF clone of Tph1 (Myc-DDK-tagged) - Mouse tryptophan hydroxylase 1 (Tph1), transcript variant 1 10 ug
$757.00
MR207141L4 Lenti ORF clone of Tph1 (mGFP-tagged) - Mouse tryptophan hydroxylase 1 (Tph1), transcript variant 1 10 ug
$757.00

Plasmid Map for MR207141

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207141
Circular map for MR207141

file-alt Citations

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