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Smoc2 (NM_022315) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207121
Smoc2 (Myc-DDK-tagged) - Mouse SPARC related modular calcium binding 2 (Smoc2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Smoc2
Synonyms 1700056C05Rik; 5430426J21Rik; Smoc2l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207121 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCGCCACAGCTGTGCTGGCTGCCGCTGCTCGCTGCCTTGCTGCCGCCGGTGCCCGCGCAGAAGT
TCTCAGCGCTCACGTTCTTGAGAGTCGATCAAGACAAAGACAGAGACTGCAGCCTGGACTGCCCAAGCTC
CCCTCAGAAGCCACTCTGTGCCTCGGACGGGAGGACCTTCCTGTCCCGATGTGAGTTCCAGCGGGCCAAG
TGCAAAGATCCACAGCTGGAGATCGCTCACCGTGGGAATTGCAAAGATGTGTCCAGGTGTGTGGCTGAGA
GGAAGTATACCCAGGAGCAGGCCCGGAAGGAGTTCCAGCAAGTGTTCATTCCAGAATGCAATGATGACGG
CACCTACAGTCAGGTCCAGTGTCACAGCTACACAGGATACTGTTGGTGTGTTACACCAAATGGAAGACCC
ATCAGTGGCACTGCTGTGGCCCACAAGACACCCAGGTGCCCCGGTTCAATAAATGAAAAGGTGCCGCAGC
GGGAAGGAGCAGGGAAAGCAGATGATGCTGCAGCCCCAGCATTGGAGACTCAGCCCCAAGGAGATGAAGA
AGATATTGCCTCACGCTACCCTACACTCTGGACCGAGCAAGTTAAGAGTCGGCAGAACAAGACCAATAAA
AATTCAGCATCCTCCTGTGATCAGGAGCATCAGTCAGCTCTTGAGGAAGCCAAGCAGCCCAAGAATGACA
ATGTAGTGATCCCCGAGTGTGCACATGGTGGTCTCTACAAGCCAGTGCAATGCCATCCATCCACCGGATA
CTGCTGGTGTGTGCTAGTGGACACTGGACGGCCCATTCCCGGGACCTCCACAAGGTATGAGCAACCTAAG
TGTGACAACACAGCCCGAGCTCACCCAGCGAAGGCCCGGGACCTGTACAAGAACAGGCCACTGCAGGGTT
GTCCTGGTGCCAAAAAGCACGAGTTTCTGACAAGTGTCCTGGATGCGCTCTCCACAGACATGGTTCATGC
CGTCTCTGACCCCTCTTCCTCTTCTGGCAGGCTGTCAGAGCCAGACCCCAGCCACACCCTGGAGGAGAGG
GTTGTACATTGGTACTTCAAGCTGCTTGATAAGAACTCTAGTGGAGACATTGGCAAGAAGGAGATCAAAC
CCTTTAAGAGGTTCCTGCGAAAGAAATCCAAGCCCAAAAAGTGTGTGAAGAAGTTTGTGGAGTACTGCGA
CATGAACAATGACAAGTCCATCACCGTGCAGGAGCTCATGGGCTGCTTGGGTGTCACCAGAGAGGAGGGT
AAAGCCAACACCAGGAAGCGCCACACCCCCAGAGGAAATGCTGAAAGTTCTTCTTCCTATAGACAGCCCA
GGAAACAAGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207121 protein sequence
Red=Cloning site Green=Tags(s)

MLPPQLCWLPLLAALLPPVPAQKFSALTFLRVDQDKDRDCSLDCPSSPQKPLCASDGRTFLSRCEFQRAK
CKDPQLEIAHRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDDGTYSQVQCHSYTGYCWCVTPNGRP
ISGTAVAHKTPRCPGSINEKVPQREGAGKADDAAAPALETQPQGDEEDIASRYPTLWTEQVKSRQNKTNK
NSASSCDQEHQSALEEAKQPKNDNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPIPGTSTRYEQPK
CDNTARAHPAKARDLYKNRPLQGCPGAKKHEFLTSVLDALSTDMVHAVSDPSSSSGRLSEPDPSHTLEER
VVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDMNNDKSITVQELMGCLGVTREEG
KANTRKRHTPRGNAESSSSYRQPRKQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022315
ORF Size 1341 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022315.1, NM_022315.2, NP_071710.1
RefSeq Size 2832 bp
RefSeq ORF 1344 bp
Locus ID 64074
UniProt ID Q8CD91
Cytogenetics 17 8.95 cM
MW 49.9 kDa
Summary Can stimulate endothelial cell proliferation, migration, as well as angiogenesis (By similarity). Promotes matrix assembly and cell adhesiveness.UniProtKB/Swiss-Prot Function
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"NM_022315" in other vectors (3)
SKU Description Size Price
MG207121 Smoc2 (tGFP-tagged) - Mouse SPARC related modular calcium binding 2 (Smoc2) 10 ug
$657.00
MR207121L3 Lenti ORF clone of Smoc2 (Myc-DDK-tagged) - Mouse SPARC related modular calcium binding 2 (Smoc2) 10 ug
$757.00
MR207121L4 Lenti ORF clone of Smoc2 (mGFP-tagged) - Mouse SPARC related modular calcium binding 2 (Smoc2) 10 ug
$757.00

Plasmid Map for MR207121

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207121
Circular map for MR207121

file-alt Citations

Ap4 is rate limiting for intestinal tumor formation by controlling the homeostasis of intestinal stem cells
Jaeckel, Kaller, Jackstadt et al
Nat Commun (2018) 9 (1), 3573 DOI: 10.1038/s41467-018-06001-x
AI Summary: OriGene contributed to this study by providing pCMV6 entry plasmids containing the Lgr5 (cat# MR219702) and Smoc2 (cat# MR207121) open reading frames, which were used to investigate the roles of these genes in intestinal stem cell regulation and tumor formation.
Snippet: The pCMV6 entry plasmids containing the Lgr5 or Smoc2 open reading frame (ORF) were obtained from Origene with the catalog number MR219702 or MR207121, respectively
FigureFigureFigure
Showing 1-1 of 1 papers.
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