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Nlrp6 (BC031139) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207117
Nlrp6 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nlrp6
Synonyms AI504961; Avr; Nalp6; Navr; Navr/Avr; Non-AVR; Pypaf5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207117 representing BC031139
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAAGTCTGCAGGCACCAATGGACCCCGGGTTCAGGGAGAGCTGAGAACGCTGTGTCGCCTGGCCA
GGGAGGGCATCCTGGATCATCATAAAGCACAATTCTCAGAAGAGGACCTGGAGAAATTGAAGCTTCGGGG
TTCCCAAGTTCAGACAATATTTCTCAACAAGAAAGAGATACCAGGAGTGCTAAAAACTGAGGTCACCTAC
CAGTTCATTGACCAGAGCTTCCAGGAGTTCTTGGCTGCATTGTCATACCTACTAGAAGCTGAGCGAACTC
CGGGGACGCCCGCAGGCGGTGTGCAGAAGCTCCTGAACTCTGACGCGGAGCTACGTGGTCATCTTGCACT
GACCACCCGATTCCTCTTTGGCCTGCTAAATACAGAGGGGCTTCGTGACATTGGAAACCATTTTGGCTGT
GTGGTGCCAGATCATGTGAAAAAGGACACCCTGCGGTGGGTACAAGGACAGAGCCACCCCAAGGGGCCAC
CAGTAGGGGCAAAAAAGACTGCTGAGCTGGAGGACATCGAGGACGCAGAGGAGGAGGAGGAGGAAGAGGA
GGACCTCAATTTTGGACTGGAGCTGCTGTACTGCCTGTATGAGACACAGGAGGAGGATTTTGTTCGACAG
GCTCTCAGCAGCCTTCCAGAGATAGTACTGGAGCGAGTCAGGTTGACCCGCATGGACCTTGAAGTTCTGA
ATTACTGCGTGCAGTGCTGCCCAGATGGCCAGGCTCTGAGACTGGTGAGCTGTGGGCTGGTGGCGGCAAA
GGAGAAGAAGAAGAAGAAGAAGAGCCTGGTGAAGCGGCTGAAGGGCTCTCAAAGCACCAAGAAACAACCC
CCAGTCTCCTTGCTGCGTCCACTCTGTGAGACAATGACTACCCCGAAATGTCATCTGAGTGTTCTGATCT
TGTCACACTGCAGACTCCCTGATGCAGTTTGCCGAGACCTTTCCGAGGCCCTGAAGGTAGCTCCTGCCCT
AAGGGAGTTGGGCCTCCTCCAGAGCCGGCTCACCAACACAGGCCTGCGTTTACTGTGCGAAGGCCTGGCT
TGGCCCAAGTGCCAGGTGAAGACACTCAGGATGCAGCTGCCTGACCTCCAAGAGGTGATCAATTACTTGG
TCATCGTGCTCCAGCAGAGCCCAGTCCTGACCACTCTGGACCTCAGTGGCTGTCAGCTGCCTGGCGTCAT
TGTGGAACCTCTGTGTGCAGCCCTGAAGCACCCTAAATGCTCCCTAAAGACCCTCAGTCTGACCTCTGTG
GAGCTGAGTGAGAACTCACTGAGGGATCTTCAAGCTGTGAAGACATCAAAGCCAGATCTGTCCATCATAT
ATTCAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207117 representing BC031139
Red=Cloning site Green=Tags(s)

MLKSAGTNGPRVQGELRTLCRLAREGILDHHKAQFSEEDLEKLKLRGSQVQTIFLNKKEIPGVLKTEVTY
QFIDQSFQEFLAALSYLLEAERTPGTPAGGVQKLLNSDAELRGHLALTTRFLFGLLNTEGLRDIGNHFGC
VVPDHVKKDTLRWVQGQSHPKGPPVGAKKTAELEDIEDAEEEEEEEEDLNFGLELLYCLYETQEEDFVRQ
ALSSLPEIVLERVRLTRMDLEVLNYCVQCCPDGQALRLVSCGLVAAKEKKKKKKSLVKRLKGSQSTKKQP
PVSLLRPLCETMTTPKCHLSVLILSHCRLPDAVCRDLSEALKVAPALRELGLLQSRLTNTGLRLLCEGLA
WPKCQVKTLRMQLPDLQEVINYLVIVLQQSPVLTTLDLSGCQLPGVIVEPLCAALKHPKCSLKTLSLTSV
ELSENSLRDLQAVKTSKPDLSIIYSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC031139
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC031139, AAH31139
RefSeq Size 3454 bp
RefSeq ORF 1340 bp
Locus ID 101613
Cytogenetics 7 F5
MW 126.6 kDa
Summary The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. provided by RefSeq, Feb 2013
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"BC031139" in other vectors (4)
SKU Description Size Price
MC218117 Nlrp6 (untagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485), (10ug) 10 ug
$503.00
MG207117 Nlrp6 (tGFP-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485) 10 ug
$657.00
MR207117L3 Lenti ORF clone of Nlrp6 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485) 10 ug
$757.00
MR207117L4 Lenti ORF clone of Nlrp6 (mGFP-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485) 10 ug
$757.00

Plasmid Map for MR207117

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207117
Circular map for MR207117

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