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Bckdha (NM_007533) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR207048
Bckdha (Myc-DDK-tagged) - Mouse branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bckdha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207048 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCGGCCAAGATCTGGAGGCCGAGCCGTGGCCTGCGCCAGGCTGCTCTTCTCCTGTTGGGACGAT
CTGGGGTTCGGGGCTTGGCTAGATCTCACCCCAGCAGGCAGCAGCAACAGCAGTTCCCATCCCTGGACGA
CAAGCCCCAGTTCCCAGGGGCCTCTGCAGAGTTTGTAGACAAGCTTGAGTTCATCCAGCCCAATGTCATC
TCCGGCATCCCCATCTACCGTGTCATGGACCGCCAGGGCCAGATCATCAACCCCAGTGAAGATCCCCACC
TGCCCCAGGAGGAGGTGCTGAAGTTCTACCGGAGCATGACGCTGCTCAACACCATGGACCGCATTCTCTA
TGAGTCCCAGCGGCAGGGCCGGATCTCCTTCTACATGACCAACTATGGCGAGGAGGGGACACATGTGGGC
AGTGCCGCTGCCTTGGAGCGCACAGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGCTCATGTACC
GGGACTACCCGCTGGAGCTGTTCATGTCCCAGTGCTACGGCAATGTGAATGACCCAGGCAAGGGACGCCA
GATGCCTGTTCACTACGGCTGCAAGGAACGCCACTTCGTCACCATTTCCTCTCCACTGGCCACGCAGATC
CCTCAGGCGGTAGGGGCAGCCTATGCTGCCAAGCGGGCCAATGCCAACCGGATTGTGATCTGTTACTTTG
GCGAGGGGGCAGCCAGTGAAGGGGATGCCCACGCCGGTTTCAATTTCGCTGCCACCCTGGAGTGTCCCAT
CATCTTCTTCTGCCGGAACAATGGCTATGCCATCTCCACACCAACCTCTGAGCAGTACCGTGGGGATGGC
ATAGCGGCTCGGGGCCCTGGGTACGGCATCATGTCAATCCGTGTGGACGGCAACGATGTGTTTGCTGTGT
ACAATGCCACTAAGGAGGCCCGACGGCGGGCTGTGGCTGAGAACCAGCCCTTCCTCATTGAGGCCATGAC
CTACAGGATTGGCCACCACAGCACCAGTGACGACAGCTCAGCGTACCGTTCGGTGGACGAGGTCAATTAC
TGGGACAAGCAGGACCACCCAATCTCGAGGCTGAGGCAGTACCTGCTGAACCAGGGTTGGTGGGATGAGG
AACAGGAGAAGGCCTGGCGGAAGCAGTCACGAAAGAAGGTCATGGAAGCCTTTGAGCAGGCTGAGCGGAA
GCTAAAGCCGAACCCAAGCCTCCTCTTCTCCGATGTGTACCAGGAGATGCCCGCCCAGCTCCGCAGGCAG
CAGGAGTCGCTGGCACGGCACCTGCAGACTTACGGGGAGCACTACCCCCTGGACCACTTTGACAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207048 protein sequence
Red=Cloning site Green=Tags(s)

MSAAKIWRPSRGLRQAALLLLGRSGVRGLARSHPSRQQQQQFPSLDDKPQFPGASAEFVDKLEFIQPNVI
SGIPIYRVMDRQGQIINPSEDPHLPQEEVLKFYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVG
SAAALERTDLVFGQYREAGVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLATQI
PQAVGAAYAAKRANANRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDG
IAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNY
WDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSRKKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRRQ
QESLARHLQTYGEHYPLDHFDK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007533
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007533.3
RefSeq Size 1773 bp
RefSeq ORF 1341 bp
Locus ID 12039
Cytogenetics 7 A3
MW 50.4 kDa
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Documents
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"NM_007533" in other vectors (4)
SKU Description Size Price
MC200515 Bckdha (untagged) - Mouse branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG207048 Bckdha (tGFP-tagged) - Mouse branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha) 10 ug
$657.00
MR207048L3 Lenti ORF clone of Bckdha (Myc-DDK-tagged) - Mouse branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR207048L4 Lenti ORF clone of Bckdha (mGFP-tagged) - Mouse branched chain ketoacid dehydrogenase E1, alpha polypeptide (Bckdha), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR207048

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207048
Circular map for MR207048

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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