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Tedc2 (NM_028056) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206952
1600002H07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1600002H07 gene (1600002H07Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tedc2
Synonyms 1600002H07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206952 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGGTGTTAAAGGAAACTACCTACTACCCGCAGACTGCGCTCACCGGCTGGTAGCGGAGCTGCAGG
GCGCCTTGGACTCCTGCGCCGATCGGCAGCGGCAGTTGGAGCGGAGCCTGCGCGTTTCCAGGAGGCTGCT
GCAGGTCTGGGAACCAGCCAGGACCCCATCTCCAGTTCCAGAAACCAAAGAAGAGGACCCAAGTCCAGCA
TGTGCACCGAGTTCTCAAGATCTCGAGGAGCTGGAACTTTTGACCCAGGCTCTGGAGAAGGCTGTACGTG
TGAGGAAAGGCGTGTCCAATGCTGGACAAAGAGACAGAACCCCCACTCTGACATCTAAGGCAGCCACTTC
TGGTGCCGCTGCCGCCTCCCATCCTCGGGCTCCCAGCCGGGGTGGCAGCCGTGTATTGGGTACAAGATCC
ACCAAGGGTATACAGCGGGCCACAGCCCCTCCCAAGGATTACCCTGAGCACAGACTACGATCAAAGGGTG
ATAAGACCCATGTAAGAACACAAGACCAAACTACTGGGTATGGACCAGACCTCAGGGACCAACAAATGAC
CCCGTCATCTGCTCATCACACCACAGAACTCTTCGCATTAAAGGAGAAGGGGACTCTCCTGCAGCTACCT
GAGGACTTCAGGAAGGCAGTGTCTCGGAATTCCTGCCTGTGGGCCCAGCTCAACTCTGCACGAACCAACG
ACTCCACAGATGCCACTAGAGCCGCCAAAACTCAGTTCCTCCACAAACTCCAGATGGCTTCAGGCTGTTC
CAGCCACAGGCCCAGTGCTACAGAGGTGGAGGCGCACGCACGGATCCTGAGGAAGGCCTGTATGCTGCTG
AGACTTCGTATGCAAAAGGAACTTGCAATAGCTCCCACTGACTGGATGCAGGAGTACCGATGCCTGCTCA
CCCTGGAAGGACTGCAGACCGTTGTAGGACAGTGTCTCCACAGGATACAGGTGCTGCAAGCAGCTGTGAC
AGAGCAACTTCCAGGAGAATGCCTTGCAGAGACGCGCACTCAGGCCTCGTCCGTTTGCGGAGGAGAGGTG
GACTCTGCCTGCAGCCCTGAGCTCCTTCTCTATGCTAGCACCGAGGAGCTGCAGACCTTGGCCACCTTGA
AGCTTCAGGTGGCCTTGCTACACCAGCAGATCCATCTAGAAAAGGTTCTGATGGCTGAACTCCTCCCTCT
GATAAACACCCAGGATCCTGGAGGACCGCCTTGGCTGGCCTTGTGTCGGGCTGCGTACAGTCTGCTCTGT
GAGGGAGGCGAACGCTTCCTCACAGTCCTTCGAGATGACCCTGCTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206952 protein sequence
Red=Cloning site Green=Tags(s)

MLGVKGNYLLPADCAHRLVAELQGALDSCADRQRQLERSLRVSRRLLQVWEPARTPSPVPETKEEDPSPA
CAPSSQDLEELELLTQALEKAVRVRKGVSNAGQRDRTPTLTSKAATSGAAAASHPRAPSRGGSRVLGTRS
TKGIQRATAPPKDYPEHRLRSKGDKTHVRTQDQTTGYGPDLRDQQMTPSSAHHTTELFALKEKGTLLQLP
EDFRKAVSRNSCLWAQLNSARTNDSTDATRAAKTQFLHKLQMASGCSSHRPSATEVEAHARILRKACMLL
RLRMQKELAIAPTDWMQEYRCLLTLEGLQTVVGQCLHRIQVLQAAVTEQLPGECLAETRTQASSVCGGEV
DSACSPELLLYASTEELQTLATLKLQVALLHQQIHLEKVLMAELLPLINTQDPGGPPWLALCRAAYSLLC
EGGERFLTVLRDDPAD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028056
ORF Size 1308 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028056.2
RefSeq Size 2179 bp
RefSeq ORF 1311 bp
Locus ID 72016
UniProt ID Q6GQV0
Cytogenetics 17 A3.3
MW 47.8 kDa
Summary Acts as a positive regulator of ciliary hedgehog signaling. Required for centriole stability.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028056" in other vectors (4)
SKU Description Size Price
MC202697 1600002H07Rik (untagged) - Mouse RIKEN cDNA 1600002H07 gene (1600002H07Rik), (10ug) 10 ug
$457.00
MG206952 1600002H07Rik (tGFP-tagged) - Mouse RIKEN cDNA 1600002H07 gene (1600002H07Rik) 10 ug
$657.00
MR206952L3 Lenti ORF clone of 1600002H07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1600002H07 gene (1600002H07Rik) 10 ug
$757.00
MR206952L4 Lenti ORF clone of 1600002H07Rik (mGFP-tagged) - Mouse RIKEN cDNA 1600002H07 gene (1600002H07Rik) 10 ug
$757.00

Plasmid Map for MR206952

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206952
Circular map for MR206952

file-alt Citations

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