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Tmprss4 (NM_145403) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206946
Tmprss4 (Myc-DDK-tagged) - Mouse transmembrane protease, serine 4 (Tmprss4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Other products for "Tmprss4 or NM_145403"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmprss4
Synonyms mCAP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206946 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCAGACAGTGGCCAACCCCTCAACAACCGTGATATTGTTCCCTTTCGCAAACCCCGAAGGCCCC
AGGAGACCTTCAAAAAGGTGGGGATCCCCATCATTGCAGTGCTGCTGAGCCTGATAGCCCTCGTGATTGT
GGCCCTTCTCATCAAGGTGATTCTGGATAAATACTACTTCATCTGCGGCAGTCCCCTGACCTTCATTCAG
AGGGGCCAGTTGTGTGACGGCCACCTTGACTGCGCCTCAGGGGAGGATGAGGAACACTGTGTCAAGGACT
TCCCTGAAAAGCCCGGAGTGGCAGTCCGGCTCTCCAAGGACAGATCCACCCTGCAGGTGCTGGATGCAGC
CACAGGGACCTGGGCCTCAGTCTGTTTCGACAACTTCACAGAAGCACTGGCCAAGACAGCCTGCAGACAG
ATGGGCTATGACAGCCAGCCCGCTTTCAGAGCAGTGGAGATCCGTCCAGATCAGAACCTCCCTGTTGCTC
AAGTCACAGGAAACAGCCAGGAACTTCAGGTGCAGAATGGAAGCAGATCCTGCCTCTCAGGCTCCCTGGT
TTCCTTGCGCTGCCTTGACTGTGGAAAGAGCCTGAAGACTCCTCGTGTGGTGGGTGGGGTGGAGGCCCCT
GTGGATTCTTGGCCGTGGCAGGTCAGCATCCAGTACAACAAGCAGCATGTCTGTGGTGGGAGCATCCTGG
ATCCCCACTGGATCCTCACAGCAGCCCACTGCTTCAGGAAGTATCTTGATGTGTCAAGCTGGAAGGTCAG
GGCAGGCTCAAACATACTGGGTAACTCTCCATCCTTGCCTGTGGCCAAGATCTTCATCGCTGAACCCAAT
CCTCTGTACCCCAAAGAGAAGGACATTGCCCTTGTTAAGCTGCAGATGCCACTCACATTCTCAGGCTCAG
TCAGGCCCATCTGCCTGCCCTTCTCTGATGAGGTGCTTGTCCCAGCCACACCAGTCTGGGTCATTGGATG
GGGCTTTACAGAAGAAAACGGAGGAAAGATGTCTGACATGCTACTGCAGGCATCAGTCCAGGTCATCGAC
AGCACACGGTGCAATGCAGAGGATGCCTACGAAGGGGAAGTGACCGCTGAGATGCTGTGTGCAGGTACCC
CACAGGGTGGCAAGGACACCTGCCAGGGTGACAGTGGTGGGCCTTTGATGTACCATTCTGACAAGTGGCA
GGTAGTAGGCATCGTGAGCTGGGGCCATGGATGCGGCGGCCCAAGTACTCCTGGAGTGTATACCAAGGTC
ACTGCCTATCTCAACTGGATCTACAATGTTCGGAAGTCTGAGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206946 protein sequence
Red=Cloning site Green=Tags(s)

MESDSGQPLNNRDIVPFRKPRRPQETFKKVGIPIIAVLLSLIALVIVALLIKVILDKYYFICGSPLTFIQ
RGQLCDGHLDCASGEDEEHCVKDFPEKPGVAVRLSKDRSTLQVLDAATGTWASVCFDNFTEALAKTACRQ
MGYDSQPAFRAVEIRPDQNLPVAQVTGNSQELQVQNGSRSCLSGSLVSLRCLDCGKSLKTPRVVGGVEAP
VDSWPWQVSIQYNKQHVCGGSILDPHWILTAAHCFRKYLDVSSWKVRAGSNILGNSPSLPVAKIFIAEPN
PLYPKEKDIALVKLQMPLTFSGSVRPICLPFSDEVLVPATPVWVIGWGFTEENGGKMSDMLLQASVQVID
STRCNAEDAYEGEVTAEMLCAGTPQGGKDTCQGDSGGPLMYHSDKWQVVGIVSWGHGCGGPSTPGVYTKV
TAYLNWIYNVRKSEM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145403
ORF Size 1305 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145403.1, NM_145403.2, NP_663378.1
RefSeq Size 2267 bp
RefSeq ORF 1308 bp
Locus ID 214523
UniProt ID Q8VCA5
Cytogenetics 9 A5.2
MW 47.5 kDa
Summary Probable protease. Seems to be capable of activating ENaC.UniProtKB/Swiss-Prot Function
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"NM_145403" in other vectors (4)
SKU Description Size Price
MC215701 Tmprss4 (untagged) - Mouse transmembrane protease, serine 4 (Tmprss4), (10ug) 10 ug
$503.00
MG206946 Tmprss4 (tGFP-tagged) - Mouse transmembrane protease, serine 4 (Tmprss4) 10 ug
$657.00
MR206946L3 Lenti ORF clone of Tmprss4 (Myc-DDK-tagged) - Mouse transmembrane protease, serine 4 (Tmprss4) 10 ug
$757.00
MR206946L4 Lenti ORF clone of Tmprss4 (mGFP-tagged) - Mouse transmembrane protease, serine 4 (Tmprss4) 10 ug
$757.00

Plasmid Map for MR206946

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206946
Circular map for MR206946

file-alt Citations

Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airway
Harbig, Mernberger, Bittel et al
J. Biol. Chem. (2020)
AI Summary: OriGene Technologies supplied pCMV6-Entry expression plasmids encoding murine proteases—including CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731)—which were used to assess the ability of these proteases to activate influenza virus hemagglutinin in vitro.
Snippet: … pCMV6-Entry expression plasmids encoding murine proteases with Cterminal Myc-DDK tag were obtained from OriGene Technologies: CFB (MR210521), HGFA (MR216475), Hepsin (MR219750), LTF (MR210170), tPA (MR208868), uPA (MR225747), tryptase ɛ (MR204321), NSP4 (MR217041), prostasin (MR223227), matriptase (MR222240), TMPRSS4 (MR206946), and TMPRSS13 (MR220731)
Showing 1-1 of 1 papers.
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