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Ints14 (NM_001077631) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206920
Vwa9 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010321M09 gene (2010321M09Rik), transcript variant 2
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $479.00
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Bulk Requests & Clone Modifications

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Other products for "Ints14 or NM_001077631"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ints14
Synonyms 2010321M09Rik; Vwa9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206920 representing NM_001077631
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCACTGTGGTGGTAATGGATGTGTCCCTGTCCATGACCCGACCTGTGTCTGTCGAGGGCTCTGAGG
AATACCAGCGGAAGCACCTGGCTGCCCATGGCCTGACGATGCTATTTGAACACATGGCCACAAATTACAA
GCTTGAATTCACAGCGCTGGTGGTGTTTTCGTCACTGTGGGAGCTGATGGTCCCCTTTACGAGAGACTAC
AACACCCTGCAGGAAGCACTGAGTAACATGGATGATTATGACAAAACCTGCTTGGAGTCTGCATTAGTCG
GCGTTTGCAATATTGTCCAGCAAGAATGGGGTGGAGCGATTCCTTGCCAGGTTGTTCTGGTGACTGATGG
CTGTCTTGGTATTGGCAGGGGGTCACTGCGACACTCCCTAGCTACTCAGAATCAAAGAAGTGAGAGCAAC
AGGTTTCCACTCCCCTTCCCTTTCCCATCTAAGTTATATGTCATGTGCATGGCGAACTTGGAGGAGCTTC
AGAGCACCGATTCCTTGGAATGCCTTGAACGACTCATAGACTTAAATAATGGTGAAGGGCAAATTTTTAC
TATTGATGGCCCCCTGTGTTTGAAAAATGTGCAATCTATGTTTGGAAAGCTGATAGATCTGGCGTACACA
CCTTTCCATGCTGTTCTCAAGTGTGGCCACCTAACGGCTGATGTACAAGTCTTCCCCAGGCCAGAGCCCT
TTGTTATAGATGAAGAAATTGACCCCATCCCTAAAGTCATTAACACAGACTTGGAAATAGTGGGCTTTAT
TGACATAGCTGATATTTCCAGCCCCCCAGTTTTGTCCAGGCATTTGGTCTTGCCCATAGCACTTAACAAA
GAAGGTGACGAGGTGGGTGCTGGCATCACTGATGACAATGAAGATGAGAATTCAGCCAATCAGATTGCTG
GCAAAATACCCAACTTCTGTGTCCTGCTCCACGGCAGCCTGAAGGTGGAAGGAATGGTGGCACTTGTCCA
ACTAGGGCCTGAATGGCATGGAATGCTCTACTCCCAAGCAGACAGCAAGAAGAAATCAAACCTCATGATG
TCTCTCTTCGAGCCTGGCCCAGAACCTCTCCCGTGGCTTGGGAAAATGGCCCAGTTGGGGCCCATCTCAG
ATGCTAAAGAAAATCCTTATGGGGAGGATGACAACAAGAGCCCATTCCCCCTGCAGCCCAAAAACAAACG
AAGTTATGCCCAGAATGTGACTGTCTGGATCAAACCAAGTGGCCTGCAGACAGATGTACAGAAGATCTTA
AGAAATGCAAGGAAGCTCCCTGAAAAAACACAGACATTCTATAAGGAGCTGAACAGGTTACGAAAAGCTG
CCTTAGCCTTTGGTTTCCTGGACCTGCTTAAAGGGGTGGCCGACATGCTGGAGAGGGAGTGTACCCTGCT
GCCGGACACAGCCCACCCAGACGCCGCATTCCAGCTCACCCACGCTGCCCAGCAGCTCAAGCTGGCCAGC
ACTGAGTATGCCATATATGACCACAACATCACACCCCTGCACACAGACTTCTCAGGGAGCAGCACTGAAA
GAATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206920 representing NM_001077631
Red=Cloning site Green=Tags(s)

MPTVVVMDVSLSMTRPVSVEGSEEYQRKHLAAHGLTMLFEHMATNYKLEFTALVVFSSLWELMVPFTRDY
NTLQEALSNMDDYDKTCLESALVGVCNIVQQEWGGAIPCQVVLVTDGCLGIGRGSLRHSLATQNQRSESN
RFPLPFPFPSKLYVMCMANLEELQSTDSLECLERLIDLNNGEGQIFTIDGPLCLKNVQSMFGKLIDLAYT
PFHAVLKCGHLTADVQVFPRPEPFVIDEEIDPIPKVINTDLEIVGFIDIADISSPPVLSRHLVLPIALNK
EGDEVGAGITDDNEDENSANQIAGKIPNFCVLLHGSLKVEGMVALVQLGPEWHGMLYSQADSKKKSNLMM
SLFEPGPEPLPWLGKMAQLGPISDAKENPYGEDDNKSPFPLQPKNKRSYAQNVTVWIKPSGLQTDVQKIL
RNARKLPEKTQTFYKELNRLRKAALAFGFLDLLKGVADMLERECTLLPDTAHPDAAFQLTHAAQQLKLAS
TEYAIYDHNITPLHTDFSGSSTERM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001077631
ORF Size 1545 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001077631.2, NP_001071099.1
RefSeq Size 2896 bp
RefSeq ORF 1548 bp
Locus ID 69882
UniProt ID Q8R3P6
Cytogenetics 9 C
MW 57.2 kDa
Summary Probable component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001077631" in other vectors (2)
SKU Description Size Price
MR206920L3 Lenti ORF clone of Vwa9 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010321M09 gene (2010321M09Rik), transcript variant 2 10 ug
$779.00
MR206920L4 Lenti ORF clone of Vwa9 (mGFP-tagged) - Mouse RIKEN cDNA 2010321M09 gene (2010321M09Rik), transcript variant 2 10 ug
$779.00

Plasmid Map for MR206920

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206920
Circular map for MR206920

file-alt Citations

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