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Dcbld1 (BC026771) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206887
Dcbld1 (Myc-DDK-tagged) - Mouse discoidin, CUB and LCCL domain containing 1 (cDNA clone MGC:30907 IMAGE:4014778)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcbld1
Synonyms 4631413K11Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206887 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACCGGGGCTGGTGGGCCGAGTGTCCTGGCGCTGCTGTTCGCCGTGTGTGCTCCGCTCCGGTTGC
AGGCGGAGGAGCTGGGTCCATATTGTGGGAGTTGGGCTGTTCCCAAAGAACTCCGGCTGAACTCAAACGA
AGTGACTGTCCTCTTCAAGAGTGGATCTCACATTTCTGGCCGGGGCTTTCTGCTGACCTACGCCAGCAGT
GACCATCCAGATTTAATAACCTGTTTGGAACGAGGCAGCCATTATTTCGAGGAAAAATACAGCAAATTCT
GCCCAGCTGGCTGTAGAGACATAGCAGGAGATATTTCTGGGAATACAAAAGATGGCTACAGAGATACCTC
TTTATTGTGCAAAGCTGCCATCCACGCAGGGATCATCACAGATGAACTAGGTGGCCACATCAACTTGCTT
CAGAGCAAAGGGATAAGTCACTATGAAGGACTCCTGGCCAATGGCGTGCTCTCCCGGCATGGTTCTTTGT
CGGAAAAGCGATTTCTTTTTACAACCCCAGGAATGAATATTACAACTGTGGCGATTCCATCAGTGATCTT
CATCGCCCTCCTTCTGACTGGAATGGGGATCTTTGCAATCTGTAGAAAGAGGAAAAAGAAAGGAAATCCA
TATGTGTCAGCTGACGCTCAGAAAACAGGCTGTTGGAAGCAGATTAAATATCCCTTTGCCAGGCATCAGT
CGACGGAATTTACCATCAGCTATGACAATGAAAAAGAGATGACACAAAAGTTGGATCTCATCACTAGTGA
TATGGCAGATTATCAGCAGCCTCTCATGATTGGCACAGGCACAGTCGCGAGAAAGGGCTCTACCTTCCGA
CCCATGGACACAGACACTGAGGAGGTCAGAGTGAACACTGAGGCCAGCGGCCACTATGACTGTCCTCACC
GCCCGGGCCGCCATGAGTACGCACTGCCTTTGACGCACTCAGAACCTGAGTATGCCACACCTATCGTGGA
GCGGCACCTGCTGCGAGCTCACACCTTCTCCACACAGAGCGGCTACCGAGTCCCTGGGCCCAGGCCCACT
CACAAACACTCCCATTCCTCTGGAGGCTTTCCTCCTGCTACAGGAGCCACCCAGATTGAAAGCTATCAGA
GGCCAGCAAGCCCCAAGCCTGTGGGTGGTGGCTATGACAAGCCTGCTGCTAGCAGCTTCTTGGACAGCAG
AGACCCAGCCTCTCAGTCACAGATGACTTCCGGGGGAGATGATGGTTATTCGGCACCCAGAACCGGTCTT
GCGCCCCTCAACCAGACGGCCATGACTGCTCTTTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206887 protein sequence
Red=Cloning site Green=Tags(s)

MGTGAGGPSVLALLFAVCAPLRLQAEELGPYCGSWAVPKELRLNSNEVTVLFKSGSHISGRGFLLTYASS
DHPDLITCLERGSHYFEEKYSKFCPAGCRDIAGDISGNTKDGYRDTSLLCKAAIHAGIITDELGGHINLL
QSKGISHYEGLLANGVLSRHGSLSEKRFLFTTPGMNITTVAIPSVIFIALLLTGMGIFAICRKRKKKGNP
YVSADAQKTGCWKQIKYPFARHQSTEFTISYDNEKEMTQKLDLITSDMADYQQPLMIGTGTVARKGSTFR
PMDTDTEEVRVNTEASGHYDCPHRPGRHEYALPLTHSEPEYATPIVERHLLRAHTFSTQSGYRVPGPRPT
HKHSHSSGGFPPATGATQIESYQRPASPKPVGGGYDKPAASSFLDSRDPASQSQMTSGGDDGYSAPRTGL
APLNQTAMTALL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC026771
ORF Size 1296 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC026771, AAH26771
RefSeq Size 2819 bp
RefSeq ORF 1298 bp
Locus ID 66686
Cytogenetics 10 B3
MW 46.8 kDa
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"BC026771" in other vectors (4)
SKU Description Size Price
MC217826 Dcbld1 (untagged) - Mouse discoidin, CUB and LCCL domain containing 1 (cDNA clone MGC:30907 IMAGE:4014778), (10ug) 10 ug
$503.00
MG206887 Dcbld1 (tGFP-tagged) - Mouse discoidin, CUB and LCCL domain containing 1 (cDNA clone MGC:30907 IMAGE:4014778) 10 ug
$657.00
MR206887L3 Lenti ORF clone of Dcbld1 (Myc-DDK-tagged) - Mouse discoidin, CUB and LCCL domain containing 1 (cDNA clone MGC:30907 IMAGE:4014778) 10 ug
$757.00
MR206887L4 Lenti ORF clone of Dcbld1 (mGFP-tagged) - Mouse discoidin, CUB and LCCL domain containing 1 (cDNA clone MGC:30907 IMAGE:4014778) 10 ug
$757.00

Plasmid Map for MR206887

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206887
Circular map for MR206887

file-alt Citations

Dynamic multi-site phosphorylation by Fyn and Abl drives the interaction between CRKL and the novel scaffolding receptors DCBLD1 and DCBLD2
Schmoker, Weinert, Kellett et al
Biochem. J. (2017)
AI Summary: OriGene contributed to this study by providing mammalian expression constructs for full-length DCBLD2 (Hs) (#RC224483) and DCBLD1 (Mm) (#MR206887), which were essential for investigating the phosphorylation-dependent interactions between these receptors and CRKL.
Snippet: Plasmids Mammalian expression constructs for full-length DCBLD2 (Hs) and DCBLD1 (Mm) in pCMV6- Entry, tagged with Flag and Myc sequences at C-termini, were obtained from Origene Page 7. (#RC224483 and #MR206887 respectively;RocNville, MD, USA). ...;... Plasmids Mammalian expression constructs for full-length DCBLD2 (Hs) and DCBLD1 (Mm) in pCMV6- Entry, tagged with Flag and Myc sequences at C-termini, were obtained from Origene Page 7. (#RC224483 and #MR206887 respectively;RocNville, MD, USA).
Showing 1-1 of 1 papers.
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