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Klhl18 (BC025563) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206865
Klhl18 (Myc-DDK-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Klhl18 or BC025563"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klhl18
Synonyms A930041K15, MGC36415
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206865 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTGTGCTGTGTTGTACGATGCAGCCAACAGCTTCATCCACCAGCACTTTGTAGAGGTGTCTCTGT
CCGAAGAGTTCCTGGCCCTGCCCTTGGAAGACGTGCTTGAGCTGGTGTCCCGGGATGAGCTGAATGTGAA
GTCAGAGGAGCAGGTTTTTGAAGCTGCATTGGCCTGGGTCAGGTATGACCGGGAGCAGAGGGGACCATGC
CTGCCCGAGCTGCTGTCCAATATCCGCCTGCCTCTTTGCCGGCCCCAGTTCTTATCAGATCGAGTGCAGC
AAGATGACCTGGTACGGTGCTGTCACAAATGCAGGGACCTGGTCGATGAAGCAAAGGACTATCATCTGAT
GCCAGAGCGCCGGCCCCACCTGCCAGCTTTCAGGACTCGGCCCCGATGCTGCACGTCCATCGCTGGGCTC
ATCTACGCTGTGGGGGGCCTCAACTCAGCAGCAAATTTTTATGCAGGTGATTCCCTGAATGTGGTGGAAG
TGTTCGACCCTATCGCCAATCGCTGGGAAAAGTGCCATCCCATGACAACAGCCCGAAGCCGTGTGGGTGT
GGCTGTGGTGAACGGGCTCCTCTATGCTATCGGGGGATATGATGGTCAGTTGCGGCTGAGCACCGTGGAG
GCCTACAATCCTGAGACGGACACATGGACCCGAGTGGGGAGCATGAATAGCAAGCGAAGTGCCATGGGGA
CAGTCGTGCTGGATGGACAGATCTACGTGTGTGGAGGCTATGACGGCAACTCCTCCCTCAACTCTGTGGA
GACCTACTCACCTGAGACGGACAAGTGGACAGTGGTGACTCCGATGAGCTCAAACCGGAGTGCTGCTGGG
GTGACAGTCTTTGAGGGCAGGATATATGTGTCAGGAGGCCACGATGGCTTGCAGATCTTCAGCAGTGTGG
AACACTACAACCACCACACGGCCACCTGGCACCCGGCAGCCAGCATGCTCAACAAGCGCTGCCGACACGG
AGCCGCCTCCCTGGGAAGCAAGATGTTTGTCTGTGGGGGCTATGATGGCTCTGGCTTCCTCAGTATTGCT
GAGATGTACAGCTCTGTGGCAGACCAGTGGTGTCTCATAGTACCCATGCACACACGCCGGAGCCGGGTCT
CCCTCGTGGCCAGCTGTGGGCGCCTCTATGCAGTGGGAGGTTACGATGGACAGTCAAACCTAAGCTCAGT
GGAGATGTACGACCCAGAGACGGACCGCTGGACATTTATGGCCCCCATGGCATGCCACGAGGGGGGGGTT
GGTGTGGGCTGCATCCCTCTTCTCACCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206865 protein sequence
Red=Cloning site Green=Tags(s)

MMCAVLYDAANSFIHQHFVEVSLSEEFLALPLEDVLELVSRDELNVKSEEQVFEAALAWVRYDREQRGPC
LPELLSNIRLPLCRPQFLSDRVQQDDLVRCCHKCRDLVDEAKDYHLMPERRPHLPAFRTRPRCCTSIAGL
IYAVGGLNSAANFYAGDSLNVVEVFDPIANRWEKCHPMTTARSRVGVAVVNGLLYAIGGYDGQLRLSTVE
AYNPETDTWTRVGSMNSKRSAMGTVVLDGQIYVCGGYDGNSSLNSVETYSPETDKWTVVTPMSSNRSAAG
VTVFEGRIYVSGGHDGLQIFSSVEHYNHHTATWHPAASMLNKRCRHGAASLGSKMFVCGGYDGSGFLSIA
EMYSSVADQWCLIVPMHTRRSRVSLVASCGRLYAVGGYDGQSNLSSVEMYDPETDRWTFMAPMACHEGGV
GVGCIPLLTI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC025563
ORF Size 1290 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC025563, AAH25563
RefSeq Size 4559 bp
RefSeq ORF 1292 bp
Locus ID 270201
Cytogenetics 9 F2
MW 47.5 kDa
Summary Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis (By similarity). The BCR(KLHL18) E3 ubiquitin ligase complex mediates the ubiquitination of AURKA leading to its activation at the centrosome which is required for initiating mitotic entry (By similarity). Regulates light- and dark-dependent alpha-transducin localization changes in rod photoreceptors through UNC119 ubiquitination and degradation (PubMed:31696965). Preferentially ubiquitinates the unphosphorylated form of UNC119 over the phosphorylated form (PubMed:31696965). In the presence of UNC119, under dark-adapted conditions alpha-transducin mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light (PubMed:31696965).UniProtKB/Swiss-Prot Function
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"BC025563" in other vectors (4)
SKU Description Size Price
MC218672 Klhl18 (untagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893), (10ug) 10 ug
$503.00
MG206865 Klhl18 (tGFP-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893) 10 ug
$657.00
MR206865L3 Lenti ORF clone of Klhl18 (Myc-DDK-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893) 10 ug
$757.00
MR206865L4 Lenti ORF clone of Klhl18 (mGFP-tagged) - Mouse kelch-like 18 (Drosophila) (cDNA clone MGC:36415 IMAGE:5322893) 10 ug
$757.00

Plasmid Map for MR206865

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206865
Circular map for MR206865

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