Nptx2 (NM_016789) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nptx2 |
| Synonyms | Narp; np2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016789 |
| ORF Size | 1287 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016789.2, NP_058069.1 |
| RefSeq Size | 2602 bp |
| RefSeq ORF | 1290 bp |
| Locus ID | 53324 |
| UniProt ID | O70340 |
| Cytogenetics | 5 84.58 cM |
| MW | 47.6 kDa |
| Summary | Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016789" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208054 | Nptx2 (untagged) - Mouse neuronal pentraxin 2 (Nptx2), (10ug) | 10 ug |
$457.00
|
|
| MG206833 | Nptx2 (tGFP-tagged) - Mouse neuronal pentraxin 2 (Nptx2) | 10 ug |
$886.00
|
|
| MR206833L3 | Lenti ORF clone of Nptx2 (Myc-DDK-tagged) - Mouse neuronal pentraxin 2 (Nptx2) | 10 ug |
$986.00
|
|
| MR206833L4 | Lenti ORF clone of Nptx2 (mGFP-tagged) - Mouse neuronal pentraxin 2 (Nptx2) | 10 ug |
$986.00
|
|
Citations
Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch
Kanehisa, Koga, Maejima et al
Nat Commun (2022) 13 (1), 2367 DOI: 10.1038/s41467-022-30089-x
Kanehisa, Koga, Maejima et al
Nat Commun (2022) 13 (1), 2367 DOI: 10.1038/s41467-022-30089-x
Cited by 21
AI Summary: OriGene’s pCMV6-Nptx2 (MR206833) plasmid was used to clone the Nptx2 gene for expression studies, enabling the investigation of NPTX2’s role in facilitating excitatory synaptic inputs onto spinal neurons in chronic itch models.
Snippet: ied from Addgene #45547) into the above-modified pZac2.1 to generate pZac2.1-GrprP-mCherry-WPRE and pZac2.1-GrprP-hM3Dq-WPRE, respectively. We cloned Nptx2 gene using pCMV6-Nptx2 (ORF) purchased from Origene (MR206833) into pZac2.1-ESYN promoter (pZac2.1-ESYN-Nptx2). The gene encoding the dominant-negative form of NPTX2 (dnNPTX2)20 was provided by Prof. Paul Worley and was subcloned into pZac2.1-E
Profiling the Gene Expression and DNA Methylation in the Mouse Brain after Ischemic Preconditioning
Cai, Zhu, Li et al
Neuroscience (2019) 406, 249-261 DOI: 10.1016/j.neuroscience.2019.03.023
Cai, Zhu, Li et al
Neuroscience (2019) 406, 249-261 DOI: 10.1016/j.neuroscience.2019.03.023
Showing 1-2 of 2 papers.
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