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Dus3l (BC052481) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206792
Dus3l (Myc-DDK-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (cDNA clone MGC:56820 IMAGE:5401486)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dus3l
Synonyms MGC56820
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206792 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGACGGCAGCGGAGAGCGGTGGTGGCGGTGACTCCGGGGTTGGCGCGTGCGAACGGGGGGTGG
CGCCCATTAAAGCTCAGTACCGCACAACCAAAGAACGGTTCCACGAATATCTGGATGGAGACAAGCAGGA
GGGTGCATGCCAGGAAGTGCCCACAGGAGACCCAGCTGAGCCAGGGGCCAAAAGAATTCGCCTGGAAGAT
GGGCAGGAAAATGGGAAGACAGAGGTGGCAATTGAATCCCGTGAAAGGCAGGTGCCCAAGAGGGCCCGTG
GCCAAAACAAAAGCCGGCCCCATGTGAAGCCAGCACACTACGACAAGGACAGGCTCTGCCCGTCCTTCCT
CCAGGAGCCGGCCACCCCATGTGCATTTGGAGACCGTTGCCGCTTCCTGCACGATGTGGGGCGCTACCTG
GAGACCAAACCGGCAGACCTGGGCCCCCGCTGCGTGCTCTTCGAAACCTTTGGCCGGTGCCCTTTCAGCA
TGACCTGTCGCTTTGCTGGAGCACACCTGGGGCCTGAGGGGCAGAACCTGGTGCAGGAAGAGGTGGTGGC
CCGCTGTGCACAGCTCCCTTCAGTGCGGAATGGCTTAGACAGGGCCCTGCAGCAGCAGCTGCGGAAGCGC
CAAGTGTGCTTTGAGCGAGCTGAGCAGGCCCTGAACCGCCTCACCCAGAGCCCCATGCCCACTGTTGTCC
CTGAGACCACTGTGGCCATGGCGACCCCAAAGCAGAATAGCTGCCATGCCCAGCTAGATACCGTAGGAGG
GGCTGGCACCCCGCAGAGCAGCCCTGTGCCAACCTGTGGCCCCTTGACAGACGAGGACGTGATCCGGCTG
CGGCCCTGTGAGAAGAAGCGGCTAGACATCAGCGGAAAGCTGTATCTGGCTCCTTTAACCACGTGTGGAA
ACCTACCCTTTCGCCGCATTTGCAAGCGCTTTGGCGCGGATGTGACCTGCGGTGAGATGGCCGTGTGTAC
GAACCTGCTCCAGGGACAGATGTCTGAGTGGGCTTTGCTCAAGCGCCACCCCTGTGAGGACATCTTTGGG
GTGCAGCTGGAGGGCGCGTTCCCTGACACCATGACCAAATGTGCCGAGCTCCTCAACCGCACCATTGACG
TGGACTTCGTGGACATCAACGTGGGCTGCCCCATCGACCTCGTCTACAAGAAGGTGATGGCTGCTCTACC
CTGGAGCCTGGGCTGGGTGTGGCCCAAGCCCGGGACCCTGCCCTGCTGGCCCTGTGTCTCAGTTTCCCCA
TCTGGACACGGAGCACAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206792 protein sequence
Red=Cloning site Green=Tags(s)

MAETAAESGGGGDSGVGACERGVAPIKAQYRTTKERFHEYLDGDKQEGACQEVPTGDPAEPGAKRIRLED
GQENGKTEVAIESRERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQEPATPCAFGDRCRFLHDVGRYL
ETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVARCAQLPSVRNGLDRALQQQLRKR
QVCFERAEQALNRLTQSPMPTVVPETTVAMATPKQNSCHAQLDTVGGAGTPQSSPVPTCGPLTDEDVIRL
RPCEKKRLDISGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFG
VQLEGAFPDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKVMAALPWSLGWVWPKPGTLPCWPCVSVSP
SGHGAH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC052481
ORF Size 1278 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052481, AAH52481
RefSeq Size 2763 bp
RefSeq ORF 1280 bp
Locus ID 224907
Cytogenetics 17 D
MW 46.7 kDa
Summary Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.UniProtKB/Swiss-Prot Function
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"BC052481" in other vectors (4)
SKU Description Size Price
MC217998 Dus3l (untagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (cDNA clone MGC:56820 IMAGE:5401486), (10ug) 10 ug
$457.00
MG206792 Dus3l (tGFP-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (cDNA clone MGC:56820 IMAGE:5401486) 10 ug
$657.00
MR206792L3 Lenti ORF clone of Dus3l (Myc-DDK-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (cDNA clone MGC:56820 IMAGE:5401486) 10 ug
$757.00
MR206792L4 Lenti ORF clone of Dus3l (mGFP-tagged) - Mouse dihydrouridine synthase 3-like (S. cerevisiae) (cDNA clone MGC:56820 IMAGE:5401486) 10 ug
$757.00

Plasmid Map for MR206792

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206792
Circular map for MR206792

file-alt Citations

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