Trim46 (BC065049) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR206757
Trim46 (Myc-DDK-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851)
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Trim46 |
| Synonyms | TRIFIC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC065049 |
| ORF Size | 1272 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC065049, AAH65049 |
| RefSeq Size | 3233 bp |
| RefSeq ORF | 1274 bp |
| Locus ID | 360213 |
| Cytogenetics | 3 F1 |
| MW | 47.2 kDa |
| Summary | Microtubule-associated protein that is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon formation, the establishment of neuronal polarity and proper neuronal migration.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC065049" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC218696 | Trim46 (untagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851), (10ug) | 10 ug |
$503.00
|
|
| MG206757 | Trim46 (tGFP-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851) | 10 ug |
$657.00
|
|
| MR206757L3 | Lenti ORF clone of Trim46 (Myc-DDK-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851) | 10 ug |
$757.00
|
|
| MR206757L4 | Lenti ORF clone of Trim46 (mGFP-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851) | 10 ug |
$757.00
|
|
Citations
Dishevelled2 Condensates control Axon Initial Segment (AIS) assembly and function through MTCL1-Dependent microtubule recruitment
Belmonte, Poirault‐Chassac, Villagomez et al
bioRxiv (2026)
Belmonte, Poirault‐Chassac, Villagomez et al
bioRxiv (2026)
Snippet: pEGFP-C1, was generously provided by Prof. I. Macara (UVA, Charlottesville, VA, USA). Full-length rat _Shank3_ cDNA has been described and used previously98. pCMV6-mTRIM46-myc-Flag was obtained from OriGene (#MR206757). pEGFP-C1-GFP-mMTCL1, pCAGGS-MCX-V5-mMTCL1 and its respective ΔN, ΔKR and ΔN/ΔKR contructs lacking MT-binding sites were a kind gift of Pr. A. Suzuki (Yokohama City University,
Mapping axon initial segment structure and function by multiplexed proximity biotinylation
Hamdan, Lim, Torii et al
Nat Commun (2020) 11 (1), 100 DOI: 10.1038/s41467-019-13658-5
Hamdan, Lim, Torii et al
Nat Commun (2020) 11 (1), 100 DOI: 10.1038/s41467-019-13658-5
Cited by 112
AI Summary: OriGene products Ndel1C and Trim46 were used as DNA templates to generate fusion constructs for proximity biotinylation experiments, enabling the mapping of axon initial segment (AIS) molecular organization in this study.
Snippet: d NF186∆FIGQY were amplified by PCR from Rat NF186 tagged with an N-terminal HA epitope tag53. pENTR11-hENO2-Ndel1C-Myc-BirA* and pENTR11-hENO2-Myc-BirA*-Ndel1C were generated by amplifying Ndel1C (Origene) and inserting it into shuttle template pENTR11-hENO2-MCS-BirA*-MCS. pENTR11-hENO2-Trim46∆C -Myc-BirA* and pENTR11-hENO2-Myc-BirA*- Trim46∆C were generated by amplifying Trim46 (Origene) a
Showing 1-2 of 2 papers.
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