Trim46 (BC065049) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG206757
Trim46 (tGFP-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trim46
Synonyms TRIFIC
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206757 representing BC065049
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAACATGGAGAAGGAACTGCTGTGCCCAGTGTGCCAAGAGATGTACAAGCAGCCACTAGTGCTGC
CTTGTACCCACAACGTGTGCCAGGCCTGTGCCCGGGAGGTTTTGGGCCAGCAGGGGTACATAGGCCATGG
TGGGGATCCGAGTTCGGAGCCCACTTCTCCTGCTTCCACCCCTTCTACCCGCAGCCCCCGCCTCTCTCGG
AGAACTCTCCCCAAGCCAGATCGCTTGGACCGGCTCCTTAAGTCAGGCTTTGGGACATACCCTGGGCGGA
AGCGTGGTGCCTTGCACCCCCAGACGATCCTATTCCCGTGCCCAGCCTGCCAAGGCGATGTGGAGCTGGG
GGAGAGGGGCCTTTCCGGGCTTTTCCGAAACCTGACCCTAGAGCGAGTGGTGGAGAGGTACCGCCAGAGT
GTGAGTGTGGGAGGAGCCATCCTGTGCCAGTTGTGCAAGCCCCCACCACTAGAGGCCACCAAGGGCTGCA
CAGAGTGTCGAGCTACCTTCTGTAACGAATGCTTCAAGCTCTTCCACCCCTGGGGCACTCAGAAGGCGCA
GCATGAGCCCACGCTACCCACTCTTTCATTCCGCCCCAAGGGTCTGATGTGTCCAGACCACAAGGAAGAG
GTGACCCACTACTGTAAGACATGTCAACGACTGGTGTGCCAACTCTGCAGAGTACGGCGTACCCACAGTG
GGCACAAGATCACACCAGTGCTCAGTGCCTACCAGGCCCTCAAGGATAAGCTAACAAAGAGCCTGGCATA
CATCTTGGGAAACCAGGACACTGTGCAGACCCAGATTTGTGAGCTGGAAGAGACCATCAGGCACACTGAG
GTGAGTGGTCAGCAAGCAAAAGAGGAGGTGTCCCAGCTGGTTCGGGGACTAGGGGCTGTGCTGGAAGAGA
AGCGGGCCTCACTGCTTCAGGCCATTGAAGAATGCCAGCAAGAGCGCTTATCCCGGCTCAGCGCCCAGAT
CCATGAACACCAGAGCCTGCTGGATGGCTCGGGTCTGGTGGGTTATGCGCAGGAAGTCCTTAAGGAAACA
GACCAGCCTTGTTTTGTACAAGCAGCCAAACAGCTGCATAACAGGATTGCCCGAGCCACTGAGGCCCTCC
AGACATTCCGGCCAGCTGCCAGCTCCTCCTTCCGCCACTGCCAGCTGGATGTGGGGCGTGAGATGAAGCT
GTTGACCGAGCTTAGCTTCCTGAGAGGCTGTGGCCACCGCGGACTCTGCTCCGGAGCACCCCAGTGCCCG
AGGCGCCCGTCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206757 representing BC065049
Red=Cloning site Green=Tags(s)

MKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSR
RTLPKPDRLDRLLKSGFGTYPGRKRGALHPQTILFPCPACQGDVELGERGLSGLFRNLTLERVVERYRQS
VSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEE
VTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLAYILGNQDTVQTQICELEETIRHTE
VSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLSRLSAQIHEHQSLLDGSGLVGYAQEVLKET
DQPCFVQAAKQLHNRIARATEALQTFRPAASSSFRHCQLDVGREMKLLTELSFLRGCGHRGLCSGAPQCP
RRPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC065049
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC065049, AAH65049
RefSeq Size 3233 bp
RefSeq ORF 1274 bp
Locus ID 360213
Cytogenetics 3 F1
Summary Microtubule-associated protein that is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon formation, the establishment of neuronal polarity and proper neuronal migration.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC065049" in other vectors (4)
SKU Description Size Price
MC218696 Trim46 (untagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851), (10ug) 10 ug
$503.00
MR206757 Trim46 (Myc-DDK-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851) 10 ug
$289.00 $457.00
MR206757L3 Lenti ORF clone of Trim46 (Myc-DDK-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851) 10 ug
$757.00
MR206757L4 Lenti ORF clone of Trim46 (mGFP-tagged) - Mouse tripartite motif protein 46 (cDNA clone MGC:86021 IMAGE:6418851) 10 ug
$757.00

Plasmid Map for MG206757

Download sequence for

Cloning scheme for MG206757

Circular map for MG206757

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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