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Ap1m1 (NM_007456) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206741
Ap1m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-1, mu subunit 1 (Ap1m1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ap1m1 or NM_007456"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ap1m1
Synonyms AA408894; Adtm; Adtm1A; AP; AP47; Clt; Cltnm; mu1; mu1A; m1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206741 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCCAGCGCCGTCTACGTACTGGATCTGAAGGGCAAGGTGCTCATCTGCAGGAACTACCGTGGGG
ATGTGGACATGTCAGAGGTGGAGCACTTCATGCCCATTCTGATGGAGAAGGAGGAGGAGGGCATGCTGTC
ACCTATCTTGGCCCATGGTGGCGTTCGTTTCATGTGGATTAAGCACAACAACCTGTACCTGGTCGCCACT
TCAAAAAAGAATGCTTGTGTGTCACTGGTGTTCTCCTTCCTCTACAAGGTGGTACAGGTCTTCTCCGAGT
ACTTTAAGGAGTTGGAGGAGGAGAGCATCCGAGACAACTTTGTCATCATCTACGAGCTGCTAGATGAGCT
CATGGACTTTGGCTACCCGCAGACCACTGACAGCAAGATCTTGCAGGAGTACATCACTCAGGAAGGCCAC
AAGCTGGAAACGGGGGCCCCTAGGCCCCCAGCCACAGTCACCAATGCTGTGTCCTGGCGTTCAGAAGGCA
TCAAGTATCGGAAGAATGAAGTATTCCTGGATGTCATTGAGGCTGTTAACCTCTTGGTCAGTGCCAATGG
CAACGTGCTGCGCAGTGAGATTGTGGGTTCCATCAAGATGCGGGTCTTCCTCTCAGGCATGCCTGAGTTA
CGCCTGGGTCTCAATGACAAGGTCCTCTTCGACAACACAGGCCGAGGGAAGAGCAAGTCAGTGGAGCTGG
AGGATGTGAAATTCCACCAGTGTGTGCGGCTGTCACGTTTTGAGAACGACCGCACTATCTCCTTCATCCC
ACCCGACGGAGAGTTTGAACTCATGTCCTACCGCCTCAACACCCATGTGAAGCCTTTGATCTGGATTGAG
TCCGTGATTGAGAAGCATTCCCACAGCCGCATTGAGTACATGGTCAAGGCCAAGAGCCAGTTCAAGAGGC
GGTCAACAGCCAACAATGTAGAGATCCATATACCAGTCCCCAACGATGCTGATTCACCCAAGTTCAAGAC
TACAGTGGGGAGTGTCAAGTGGGTCCCTGAAAACAGTGAGATCGTGTGGTCCGTCAAGTCCTTTCCGGGT
GGCAAGGAGTACCTGATGCGGGCCCACTTTGGCCTTCCCAGTGTGGAAGCTGAAGACAAGGAGGGAAAGC
CCCCCATCAGCGTCAAGTTTGAGATCCCCTATTTCACTACCTCTGGCATCCAGGTGCGCTACCTGAAAAT
CATTGAGAAGAGTGGGTACCAGGCCCTGCCCTGGGTACGATATATCACACAGAACGGAGATTATCAGCTC
CGGACCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206741 protein sequence
Red=Cloning site Green=Tags(s)

MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVAT
SKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGH
KLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIEAVNLLVSANGNVLRSEIVGSIKMRVFLSGMPEL
RLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIE
SVIEKHSHSRIEYMVKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSVKSFPG
GKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQL
RTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007456
ORF Size 1269 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007456.5
RefSeq Size 1964 bp
RefSeq ORF 1272 bp
Locus ID 11767
UniProt ID P35585
Cytogenetics 8 34.92 cM
MW 48.5 kDa
Summary This gene encodes the mu-1 subunit of the scaffolding adapter protein complex AP-1 and is a member of the mu adaptin family. The AP-1 complex, which consists of 4 subunits (mu-adaptin, beta-prime adaptin, gamma-adaptin, and the small chain adaptin), is one of the predominant coat proteins of membrane vesicles involved in eukaryotic post-Golgi trafficking. The AP-1 complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. AP-1 complex subunit mu-1 and other mu-adaptins select cargo proteins bearing sequence-specific sorting motifs. provided by RefSeq, Jul 2016
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"NM_007456" in other vectors (4)
SKU Description Size Price
MC205598 Ap1m1 (untagged) - Mouse adaptor-related protein complex AP-1, mu subunit 1 (Ap1m1), (10ug) 10 ug
$457.00
MG206741 Ap1m1 (tGFP-tagged) - Mouse adaptor-related protein complex AP-1, mu subunit 1 (Ap1m1) 10 ug
$657.00
MR206741L3 Lenti ORF clone of Ap1m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-1, mu subunit 1 (Ap1m1) 10 ug
$757.00
MR206741L4 Lenti ORF clone of Ap1m1 (mGFP-tagged) - Mouse adaptor-related protein complex AP-1, mu subunit 1 (Ap1m1) 10 ug
$757.00

Plasmid Map for MR206741

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206741
Circular map for MR206741

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