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Ccna2 (NM_009828) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206720
Ccna2 (Myc-DDK-tagged) - Mouse cyclin A2 (Ccna2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccna2
Synonyms AA408589; Ccn-1; Ccn1; Ccna; Cyca; CycA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206720 representing NM_009828
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGCACCTCGAGGCATTCGGGTCGCGATGCGGGCTCAGCCCTGCTCTCGCTGCATCAGGAAGACC
AAGAGAATGTCAACCCCGAAAAACTGGCGCCAGCCCAGCAGCCGCGGGCGCAGGCGGTGCTGAAGGCCGG
GAACGTGCGTGGACCCGCGCCGCAGCAGAAGCTCAAGACTCGACGGGTTGCTCCTCTTAAGGACCTTCCT
ATAAACGATGAGCATGTCACTGCTGGTCCTTCATGGAAAGCAGTCAGTAAACAGCCTGCCTTCACCATTC
ATGTGGATGAAGCAGAAGAGACTCAGAAGAGGCCAGCTGAGCTTAAAGAAACAGAGTGTGAAGATGCCCT
GGCTTTTAATGCAGCTGTCTCTTTACCCGGAGCAAGAAAACCACTGACACCTCTTGACTATCCAATGGAT
GGCAGTTTTGAATCACCACATGCTATGGATATGTCTATTGTCTTAGAAGACAAGCCAGTGAACGTTAATG
AAGTACCTGACTATCAAGAAGACATTCACACGTACCTTAGGGAAATGGAGGTTAAATGTAAACCTAAAGT
GGGTTACATGAAGAGGCAGCCAGACATCACTAACAGCATGAGGGCCATCCTTGTGGACTGGCTGGTTGAG
GTGGGAGAAGAATATAAACTACAGAATGAGACCCTGCATTTGGCTGTGAACTACATTGATAGATTCCTCT
CCTCCATGTCTGTGTTAAGAGGGAAGCTTCAGCTTGTAGGCACGGCTGCTATGCTGCTAGCTTCGAAGTT
TGAAGAAATATACCCCCCAGAAGTAGCAGAGTTTGTGTATATTACAGACGATACCTATTCCAAGAAGCAG
GTTCTGAGAATGGAGCACCTAGTATTGAAAGTCCTTGCTTTTGACTTGGCTGCACCAACAGTAAATCAGT
TCCTTACCCAGTACTTCCTGCACCTGCAGCCTGCAAACTGTAAGGTTGAAAGCTTAGCAATGTTTTTGGG
AGAACTGAGTTTGATAGATGCTGACCCGTACCTTAAGTACCTGCCTTCACTCATTGCTGGAGCTGCCTTC
CACTTGGCTCTCTACACAGTCACAGGACAGAGCTGGCCTGAGTCATTGGCACAACAGACTGGATATACCC
TGGAGAGTCTTAAGCCTTGTCTTGTGGACCTTCACCAGACCTACCTCAAAGCGCCACAACATGCCCAACA
GTCAATACGGGAAAAGTACAAGCATTCAAAATATCACAGTGTTTCTCTTCTCAACCCACCAGAGACACTA
AGTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009828
ORF Size 1266 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009828.3
RefSeq Size 2796 bp
RefSeq ORF 1269 bp
Locus ID 12428
UniProt ID P51943
Cytogenetics 3 17.67 cM
MW 47.7 kDa
Summary Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 and CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.UniProtKB/Swiss-Prot Function
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"NM_009828" in other vectors (4)
SKU Description Size Price
MC202654 Ccna2 (untagged) - Mouse cyclin A2 (Ccna2), (10ug) 10 ug
$457.00
MG206720 Ccna2 (tGFP-tagged) - Mouse cyclin A2 (Ccna2) 10 ug
$657.00
MR206720L3 Lenti ORF clone of Ccna2 (Myc-DDK-tagged) - Mouse cyclin A2 (Ccna2) 10 ug
$757.00
MR206720L4 Lenti ORF clone of Ccna2 (mGFP-tagged) - Mouse cyclin A2 (Ccna2) 10 ug
$757.00

Plasmid Map for MR206720

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206720
Circular map for MR206720

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