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Tmlhe (NM_138758) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206690
Tmlhe (Myc-DDK-tagged) - Mouse trimethyllysine hydroxylase, epsilon (Tmlhe), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tmlhe or NM_138758"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmlhe
Synonyms Bbox2; D430017M14Rik; TMLH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206690 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGTACCATAAGTTGTTGCACCAACAAAGCAGGCTCCGTAATTTGATGAAGAGAGGAAACATAGCTC
AAGGTCTTCATCTGTCCAACTTTAAAAGTTTATTTTCATCATCCATCCATTGGTGCCATACAACTTCCAA
GTCTGTGAACTGTACTTGGCACCAACATGAAGATCATTTGGAACTGCAATATGCTGGCACTGTGATGCGC
TTTGATTATGTCTGGCTTCGAGACCATTGCCGCTCAGCATCATGCTATAATTCTAAGACTCACCAGCGGA
GCCTGGACACTGCCAGTGTGGATTTATGTATTAAGCCAAAGACAGTTCATCTGGATGAAACTATGCTCTT
TTTCACTTGGCCAGATGGTCATGTGACTAGATATGATCTGGATTGGCTGGTGAAAAACAGTTATGAAGGG
CAGAAACAAAAGGTTATCCAACCTAGAATATTATGGAATTCTAAACTCTACCAGCAAGCCCAAGTTCCAT
CTGTTGATTTCCAGTGCTTTTTAGAAACAAATGAAGGGTTGAAGAAGTTTCTGCAGAACTTTTTGCTGTA
TGGAATTGCATTTGTAGAAAATGTCCCTCCCACTGAAGAGCATACAGAAAAATTGGCAGAAAGGATTAGC
TTAATCAGAGAAACCATCTATGGAAGGATGTGGTATTTCACTTCAGATTTTTCCAGAGGTGACACTGCAT
ACACCAAGCTAGCTCTAGACCGGCATACTGACACTACCTACTTTCAAGAGCCCTGTGGCATTCAAGTATT
TCATTGTCTTAAGCATGAAGGAACAGGTGGTAGAACACTGTTAGTGGATGGATTCTATGCAGCACAACAA
GTACTTCAAAAAGCACCTGAGGAATTTGAACTCCTCAGCAAAGTACCATTGAAGCATGAATATATTGAAA
ATGTTGGACAATGTCACAATCACATGATTGGGGTTGGGCCCATCTTAAATATTTACCCATGGAATAAAGA
GCTGTATTTGATCAGGTACAACAACTATGACAGGGCTGTCATCAACACTGTGCCTTATGATGTTGTCCAT
CGTTGGTATACAGCACACCGAACCCTAACAACTGAATTGAGAAGGCCTGAGAATGAGCTTTGGGTCAAAT
TGAAGCCTGGAAAGGTACTATTTATAGACAACTGGCGAGTACTTCATGGCAGGGAATCATTCACTGGCTA
CCGTCAACTCTGTGGCTGCTATTTAACTAGAGATGATGTATTGAATACTGCTCGTCTCCTGGGTCTTCAT
GCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206690 protein sequence
Red=Cloning site Green=Tags(s)

MWYHKLLHQQSRLRNLMKRGNIAQGLHLSNFKSLFSSSIHWCHTTSKSVNCTWHQHEDHLELQYAGTVMR
FDYVWLRDHCRSASCYNSKTHQRSLDTASVDLCIKPKTVHLDETMLFFTWPDGHVTRYDLDWLVKNSYEG
QKQKVIQPRILWNSKLYQQAQVPSVDFQCFLETNEGLKKFLQNFLLYGIAFVENVPPTEEHTEKLAERIS
LIRETIYGRMWYFTSDFSRGDTAYTKLALDRHTDTTYFQEPCGIQVFHCLKHEGTGGRTLLVDGFYAAQQ
VLQKAPEEFELLSKVPLKHEYIENVGQCHNHMIGVGPILNIYPWNKELYLIRYNNYDRAVINTVPYDVVH
RWYTAHRTLTTELRRPENELWVKLKPGKVLFIDNWRVLHGRESFTGYRQLCGCYLTRDDVLNTARLLGLH
A

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138758
ORF Size 1263 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138758.2
RefSeq Size 2264 bp
RefSeq ORF 1266 bp
Locus ID 192289
UniProt ID Q91ZE0
Cytogenetics X
MW 49.6 kDa
Summary Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML).UniProtKB/Swiss-Prot Function
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"NM_138758" in other vectors (4)
SKU Description Size Price
MC212444 Tmlhe (untagged) - Mouse trimethyllysine hydroxylase, epsilon (Tmlhe), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG206690 Tmlhe (tGFP-tagged) - Mouse trimethyllysine hydroxylase, epsilon (Tmlhe) 10 ug
$657.00
MR206690L3 Lenti ORF clone of Tmlhe (Myc-DDK-tagged) - Mouse trimethyllysine hydroxylase, epsilon (Tmlhe), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206690L4 Lenti ORF clone of Tmlhe (mGFP-tagged) - Mouse trimethyllysine hydroxylase, epsilon (Tmlhe), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206690

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206690
Circular map for MR206690

file-alt Citations

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