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Brf2 (NM_025686) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206680
Brf2 (Myc-DDK-tagged) - Mouse BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (Brf2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Brf2
Synonyms 2700059M06Rik; 5730512K07Rik; AU018504; AU022602; BRFU; TFIIIB50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206680 representing NM_025686
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGAACGGAAGCCGCTGCCCGGATTGCGGCTCCTCCGAGCTCGTGGAAGATTCGCATTATTCTCAGA
GCCAGCTGGTGTGCTCCGACTGCGGCTGCGTGGTCACCGAAGGGGTCCTAACTACCACCTTTAGCGACGA
GGGCAACTTCAGGGAAGTGACATATTCTCGAAGCACTGGGGAAAACGAACAAGTTAGTCGCTGCCAGCAA
CGAGATCTCCGTCGAGTAAGAGACCTGTGTCGGATTCTGAAGCTGCCGCTAACGTTTGAGGATACAGCAA
TCTCCTACTACCAGAAGGCATATCAGCTGTCTGGGATCCGAGCTGCCAGGCTGCAGAAGAAGGAAGTGTT
AGTCGGATGCTGTGTTTTAATCACCTGTCGGCAACATAACTGGCCCCTCACCATGGGAACCATCTGCACA
CTGCTCTATGCCGATCTGGACTTGTTTTCTGGCACCTACATGCAGATGGTAAAGCTCCTGGGGCTGGATG
TGCCCTCCCTGTGCCTGGCAGACTTGGTGAAGTCTTACTGCAGCAGCTTCAAACTGTTCCAAGCGTCCCC
GTCCGTGCCAGCCAAATACGTGGAAGACAAAGACAAGATGCTGTCTCGAACCTTGCTGTTGGTGGAGCTA
GCAGATGAGACGTGGCTAGTGACTGGACGGCATCCCTTGCCCATCATCACTGCAGCCACCTTCCTGGCAT
GGCAGTCTCTGCGGCCTTCAGACCGGCTGACCTGCTCTCTTGCCCAGTTTTGTAAATTGGCGAATGTGGA
CCTGCCCTACCCAGCTGCCTCTCGCCTGCAGGAGCTCCTGGCCGTGCTCCTTCAGATGGCCGGCCAGCTG
GCCTGGCTACAGGTTCTAAAACTTAACAAGCGGTCGGTGGTGAAACACATTGGTGACCTTCTCCAGCACC
GCCACATGCTGGTCCGAACGGCTTTTCGAGATGGAACAGCAGAAGTGGAGACCCAGCAGCAGCAGCAGCA
GCAGCAGGGACAAGGACAAGGACAGCAGGACGAGGTGGGGGATGGTCCCTTCGATTTGCCCAAGAGGAAA
CGTCCCGCCAGTCCCACTCCCCTCCTGCCCCCCTGCATGTTGAAGCCCCCCAAGCGGACCCACACCTTGC
CCCCCGAATCTGCAGTGACTGGCGATGAGGATATTTCAGATAGTGAAATAGAGCAGTATTTGCGCACCCC
TCAGGAAGTTAGAGACTTTGAGAGAGCCCAAGCTGCTAGCCAGGCAGCCATGCGTGTCCCTAACCCTCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025686
ORF Size 1260 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025686.2, NP_079962.1
RefSeq Size 1383 bp
RefSeq ORF 1263 bp
Locus ID 66653
UniProt ID Q3UAW9
Cytogenetics 8 A2
MW 47.4 kDa
Summary General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.UniProtKB/Swiss-Prot Function
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"NM_025686" in other vectors (4)
SKU Description Size Price
MC210411 Brf2 (untagged) - Mouse BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (Brf2), (10ug) 10 ug
$503.00
MG206680 Brf2 (tGFP-tagged) - Mouse BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (Brf2) 10 ug
$657.00
MR206680L3 Lenti ORF clone of Brf2 (Myc-DDK-tagged) - Mouse BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (Brf2) 10 ug
$757.00
MR206680L4 Lenti ORF clone of Brf2 (mGFP-tagged) - Mouse BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (Brf2) 10 ug
$757.00

Plasmid Map for MR206680

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206680
Circular map for MR206680

file-alt Citations

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