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Egln2 (NM_053208) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206645
Egln2 (Myc-DDK-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Egln2
Synonyms 0610011A13Rik; C85656; Hif-p4h-1; Ier4; Phd1; SM-20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206645 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAGCCCGTGCCAGCCGCAGGCCCTGAATCAAGCTCTCCCTCAGTTGCCAGGGTCTGTGTCAGAGT
CCTTGGAGTCTAGCCGAGCCAGAATGGGGGTGGAGAGTTACCTGCCCTGCCCTCTGCTCCCGGCCTATCA
CCGTCCAGGAGCATCTGGGGAGGCCTCGGCTGGCAATGGGACCCCCAGAACCACAGCCACTGCTACTACG
ACCACTGCCAGTCCCCTGCGGGAGGGCTTTGGTGGGCAGGATGGTGGTGAGCTTTGGCCACTGCAGAGTG
AAGGTGCTGCTGCGTTGGTCACCAAGGAGTGCCAGCGACTGGCGGCCCAGGGTGCCCGGCCTGAGGCCCC
CAAACGGAAGTGGGCCAAGGATGGTGGGGATGCCCCTTCACCCAGCAAGCGACCGTGGGCCAGGCAAGAG
AACCAGGAGGCCAAGGGGGAAAGTGGTATGGGCTGTGACAGCGGTGCCAGCAACAGCAGCAGCAGCAGCA
GCAACACTACCAGTAGCAGTGGCGAGGCAAGTGCTAGGCTGAGGGAGGAAGTCCAGCCCTCTGCACCTGA
GCGCCTGGCCCTGGACTATATTGTGCCTTGCATGCGGTACTATGGTATCTGTGTCAAGGACAACTTCTTG
GGGGCAGTACTGGGTGGCCGTGTGCTGGCTGAGGTGGAAGCCCTGAAGTGGGGCGGGCGTCTTCGTGATG
GGCAACTAGTGAGCCAGCGGGCGATCCCACCGCGCAGCATTCGTGGGGACCAGATTGCCTGGGTAGAAGG
TCACGAGCCAGGCTGCCGGAGCATTGGTGCCCTCATGGCTCACGTGGACGCAGTAATCCGCCACTGTGCA
GGGCGGCTGGGCAACTACGTCATCAATGGGCGCACCAAGGCCATGGTGGCGTGTTATCCAGGCAATGGGC
TCGGGTACGTGAGGCATGTTGACAATCCCCACGGCGATGGGCGCTGCATCACCTGTATCTATTACCTGAA
TCAGAACTGGGATGTTAAGGTGCATGGCGGCCTGCTGCAGATCTTCCCCGAGGGTCGGCCAGTGGTAGCC
AACATCGAGCCACTCTTTGACCGGTTGCTCATTTTCTGGTCTGACCGACGGAACCCACATGAGGTGAAGC
CAGCCTATGCCACCAGGTACGCCATCACTGTCTGGTATTTTGATGCCAAGGAACGGGCAGCAGCCAGAGA
CAAGTATCAGCTAGCATCGGGACAGAAAGGTGTTCAAGTACCAGTATCACAGCCAACTACACCTACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206645 protein sequence
Red=Cloning site Green=Tags(s)

MDSPCQPQALNQALPQLPGSVSESLESSRARMGVESYLPCPLLPAYHRPGASGEASAGNGTPRTTATATT
TTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQE
NQEAKGESGMGCDSGASNSSSSSSNTTSSSGEASARLREEVQPSAPERLALDYIVPCMRYYGICVKDNFL
GAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCA
GRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVA
NIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPTTPT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053208
ORF Size 1257 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053208.4
RefSeq Size 2124 bp
RefSeq ORF 1260 bp
Locus ID 112406
UniProt ID Q91YE2
Cytogenetics 7 15.83 cM
MW 45.1 kDa
Summary Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_053208" in other vectors (4)
SKU Description Size Price
MC201719 Egln2 (untagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2), (10ug) 10 ug
$457.00
MG206645 Egln2 (tGFP-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2) 10 ug
$657.00
MR206645L3 Lenti ORF clone of Egln2 (Myc-DDK-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2) 10 ug
$757.00
MR206645L4 Lenti ORF clone of Egln2 (mGFP-tagged) - Mouse EGL nine homolog 2 (C. elegans) (Egln2) 10 ug
$757.00

Plasmid Map for MR206645

Download sequence for

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Cloning scheme for MR206645
Circular map for MR206645

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