Sgms1 (NM_144792) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR206639
Sgms1 (Myc-DDK-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sgms1 |
| Synonyms | 9530058O11Rik; AI841905; C80702; Mob; Sms1; Sor1; Tmem23 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_144792 |
| ORF Size | 1257 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_144792.5 |
| RefSeq Size | 3624 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 208449 |
| UniProt ID | Q8VCQ6 |
| Cytogenetics | 19 C1 |
| MW | 49.3 kDa |
| Summary | Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types (By similarity). Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_144792" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC202951 | Sgms1 (untagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2, (10ug) | 10 ug |
$457.00
|
|
| MG206639 | Sgms1 (tGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1) | 10 ug |
$886.00
|
|
| MR206639L3 | Lenti ORF clone of Sgms1 (Myc-DDK-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 | 10 ug |
$986.00
|
|
| MR206639L4 | Lenti ORF clone of Sgms1 (mGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 | 10 ug |
$986.00
|
|
Citations
Visualizing intra-Golgi localization and transport by side-averaging Golgi ministacks
Tie, Mahajan, Lu
J Cell Biol (2022) 221 (6) DOI: 10.1083/jcb.202109114
Tie, Mahajan, Lu
J Cell Biol (2022) 221 (6) DOI: 10.1083/jcb.202109114
Cited by 43
AI Summary: OriGene products B4GALT7-Myc (#RC200258), B3GALT6-Myc (#MR204731), POMGNT1-Myc (#RC200176), SLC35C1-Myc (#RC200101), and SMS1-Myc (#MR206639) were used as tagged Golgi protein constructs to visualize and analyze the cisternal organization and intra-Golgi transport in this study.
Snippet: … B4GALT7-Myc (#RC200258), B3GALT6-Myc (#MR204731), POMGNT1-Myc (#RC200176), SLC35C1-Myc (#RC200101), and SMS1-Myc (#MR206639) were purchased from Origene. …B4GALT7-Myc (#RC200258), B3GALT6-Myc (#MR204731), POMGNT1-Myc (#RC200176), SLC35C1-Myc (#RC200101), and SMS1-Myc (#MR206639) were purchased from Origene. …
Showing 1-1 of 1 papers.
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