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Sgms1 (NM_144792) Mouse Untagged Clone

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SKU
MC202951
Sgms1 (untagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sgms1
Synonyms 9530058O11Rik; AI841905; C80702; Mob; Sms1; Sor1; Tmem23
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC019443 sequence for NM_144792
AGTCCCTTGAGCTCCGTGTCCTCTGCGTCTGGCTGTGCTTCCCAGAGGCGGCCTGTGGGGACCGCTGGCC ACTCCCAACCGCTGGCCAGCTGCACGCAGAGACCCGCGTAAACTTGTCAGGAACTTTCCAGGAGGCTGCC TTTGGGAGGAGAGAAGGAAGAATTCTGTTCAGAAATGAGGCGAACGAATGTTTGGACACCGTTGCATGAA GTGTTTTAAAACTGCCCTGACCATCTTGCCAAATGAGTCTCTGCTCATGAGGCCCAACAAGATGAACCAG CCAAGGAGGGATAGTGTCCTGCCCTGTACGAACAGTGACTGCTGACGTCTGAGAGCAAGCTGGGGGTACT GAATGTTGTCTGCCAGGACCATGAAGGAAGTGGTTTACTGGTCACCCAAGAAGGTGGCAGACTGGCTGCT GGAGAATGCTATGCCAGAATACTGTGAGCCTCTGGAGCACTTCACAGGCCAGGACTTAATCAACCTAACC CAAGAGGATTTCAAAAAACCCCCACTGTACCGAGTCTCCTCTGACAATGGGCAGCGACTCTTAGACATGA TAGAGACCCTGAAGATGGAGCACCATATGGAAGCACACAAGAATGGCCACGCCAACGGACACCTCAGCAT TGGCGTTGACATTCCCAACCCCGATGGCAGCTTCAGCATCAAGACTAAACCCAACGGAATGCCAAATGGG TTTAGGAAAGAGATGATCAAGATCCCCATGCCAGAACCGGAGCGCTCCCAGTATCCCATGGAGTGGGGCA AGACTTTCCTGGCCTTTCTTTATGCACTTTCCTGTTTTGTTCTCACTACAGTGATGATCTCGGTCGTCCA TGAACGAGTACCTCCTAAGGAGGTGCAGCCTCCACTACCGGACACGTTTTTTGACCATTTTAACCGGGTG CAGTGGGCGTTTTCTATTTGCGAAATTAACGGCATGATCCTTGTAGGACTCTGGCTATTTCAGTGGCTGC TCTTAAAATACAAGTCTATTATTAGCAGAAGATTTTTCTGCATAGTTGGCACGCTGTACCTGTATCGGTG TATTACAATGTATGTAACTACACTCCCAGTACCTGGCATGCATTTCAACTGTTCTCCGAAGCTCTTTGGA GACTGGGAAGCTCAAGTGCGGAGAATAATGAAGCTCATTGCTGGAGGTGGCTTATCCATCACAGGCTCGC ACAACATGTGTGGCGACTATCTGTACAGTGGCCACACGGTCATGCTAACGCTCACCTACCTATTTATCAA AGAGTATTCTCCTCGGCGGCTCTGGTGGTACCACTGGATTTGCTGGCTCCTCAGCGTCGTTGGAATCTTC TGTATTCTCTTAGCGCATGACCACTACACTGTGGACGTGGTGGTGGCCTACTACATCACCACAAGACTCT TCTGGTGGTATCACACGATGGCCAATCAGCAAGTGCTTAAGGAAGCCTCCCAGATGAACCTCCTGGCCAG GGTGTGGTGGTACAGGCCATTTCAGTACTTTGAAAAGAATGTCCAAGGAATTGTACCTCGATCTTACCAT TGGCCCTTCCCCTGGCCGGTAGTCCACCTTAGTAGGCAAGTTAAATATAGCCGGCTGGTAAACGACACAT AACAGCTCTGCCGACTAGGGAATGAAGAACAGTCCGAAGTGCTAAGAACTCCATGACAGAAGATGCCATA AAGTAACCACCTCCCCCTGCTATCTCTCAAGTTACCTTGACTTAACCTATTGCGTTACCTGCTCAGCACT GTGATCTTTTTTTTTCTCTCCAAAGGACCCGCGTTGGACAACAATAAAGAAAAGTTCCCACATCATGCAC TGTAGACATTTCCTGTTGTTAAGCTTGGGGCTTCTATAACAGGCAGCCACCGTGTCCCTCTGTGTCATTC AGCCGCACCACCGTGTGCTTCTGTATCCTAAGCTCCGGCCTTTCCAGCCATATCTGGAAATAAAGTGTGT TATTTTCTTGGGAAAACATACTTTTCATATCTCTTGCCCCAAAGACAGGGCTGTAAGCCATTTTCTAGTA AATGTCTCTATGAAGGGTCCTAAGTGTCTGCATGGGAGTTGATTTAGAATCTCTCTGCCTATTGCACTGA TGCTCGGCTGAAGACGATAGGTCCAGAGAGGTTTGCTCGCCTGGGGTTTTATACAGTCAGCAGAGACGGT GTATCAAAAAGTGCAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_144792
Insert Size 1260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019443, AAH19443
RefSeq Size 2199 bp
RefSeq ORF 1260 bp
Locus ID 208449
UniProt ID Q8VCQ6
Cytogenetics 19 C1
Summary Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types (By similarity). Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2, also known as Sms1alpha1) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.
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Documents
Resources
"NM_144792" in other vectors (4)
SKU Description Size Price
MG206639 Sgms1 (tGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1) 10 ug
$886.00
MR206639 Sgms1 (Myc-DDK-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 10 ug
$686.00
MR206639L3 Lenti ORF clone of Sgms1 (Myc-DDK-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 10 ug
$986.00
MR206639L4 Lenti ORF clone of Sgms1 (mGFP-tagged) - Mouse sphingomyelin synthase 1 (Sgms1), transcript variant 2 10 ug
$986.00

file-alt Citations

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