Preb (NM_016703) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206570
Preb (Myc-DDK-tagged) - Mouse prolactin regulatory element binding (Preb)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Preb
Synonyms C85705
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206570 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCGGCGCCGGGGTGTGGAGCTGTACCGGGCCCCGTTCCCGTTGTACGCGCTTCGGATAGACCCCA
AGACTGGGCTGCTCATCGCTGCGGGCGGAGGAGGAGCTGCCAAGACCGGCATAAAGAATGGCGTGCATTT
TCTGCAGCTAGAGCTGATCAACGGGTGCCTGAGCGCTTCCTTGCTGCACTCTCATGACACGGAGACACGG
GCCACCATGAATTTGGCGCTTGCTGGTGACATTCTTGCTGCCGGACAGGATGCCCAGTGTCAGCTTCTTC
GTTTCCAGGTCCATCAACAGAAGGGCAGTAAAGCGGAGAAGTCAGGTTCCAAGGAGCAGGGACCTCGACA
GAGAAAGGGGGCTCCTCCAGCAGAGAAGAAATCGGGAGCACAAGTTCACCCCGAAGGGGTTGAACTCAAA
GTAAAGAATTTGGAGGCAGTACAGACAGACTTCAGCAATGAACCGCTGCAGAAAGTTGTGTGCTTCAACC
ATGATAACACCCTGCTTGCCACTGGAGGAACTGATGGTCATGTTCGTGTCTGGAAGGTACCTAGCCTGGA
GAAAGTTCTGGAGTTCAAAGCCCATGAAGGGGAGATTGGAGATTTGACTTTGGGTCCTGATGGCAAGCTG
GTTACAGTGGGCTGGGACTTTAAGGCCTCTGTGTGGCAGAAGGATCAACTGGTGACACAGCTACAGTGGC
AAGAGAATGGACCAGCCTCTTCTAATACACCATACCGCTACCAGGCCTGCAGGTTTGGGCAGGTTCCAGA
TCAGCTCGGTGGGCTGCGACTCTTCACAGTGCAGATACCCCACAAGCGCCTACGTCAGCCCCCACCCTGC
TACCTCACAGCCTGGGACAGTTCCACCTTCTTGCCTCTTCGGACCAGATCCTGTGGCCATGAGGTCATTT
CCTGCCTCAGTGTCAGTGATTCGGGTACCTTCCTAGGCCTAGGCACGGTCACTGGCTCTGTCGCCATCTA
CATAGCTTTCTCTCTCCAGCGCCTGTATTATGTGAAGGAGGCCCATGGCATTGTGGTAACAGATGTGACC
TTTCTACCTGAGAAGGGTTGTGGTCCAAAGCTCCTTGGGCCCCATGAAACTGCTCTTTTCTCTGTGGCTG
TGGATAGTCGTTGCCAGTTGCACCTGCTGCCTTCACGGCGGAGTGTTCCTGTATGGCTTCTGCTCCTGCT
GTGTGTCGGCCTTATTATTGTGACCATCCTGCTGCTCCAGACTGCCTTCCCGGGATTTCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206570 protein sequence
Red=Cloning site Green=Tags(s)

MGRRRGVELYRAPFPLYALRIDPKTGLLIAAGGGGAAKTGIKNGVHFLQLELINGCLSASLLHSHDTETR
ATMNLALAGDILAAGQDAQCQLLRFQVHQQKGSKAEKSGSKEQGPRQRKGAPPAEKKSGAQVHPEGVELK
VKNLEAVQTDFSNEPLQKVVCFNHDNTLLATGGTDGHVRVWKVPSLEKVLEFKAHEGEIGDLTLGPDGKL
VTVGWDFKASVWQKDQLVTQLQWQENGPASSNTPYRYQACRFGQVPDQLGGLRLFTVQIPHKRLRQPPPC
YLTAWDSSTFLPLRTRSCGHEVISCLSVSDSGTFLGLGTVTGSVAIYIAFSLQRLYYVKEAHGIVVTDVT
FLPEKGCGPKLLGPHETALFSVAVDSRCQLHLLPSRRSVPVWLLLLLCVGLIIVTILLLQTAFPGFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016703
ORF Size 1251 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016703.3
RefSeq Size 5471 bp
RefSeq ORF 1254 bp
Locus ID 50907
UniProt ID Q9WUQ2
Cytogenetics 5 B1
MW 45.4 kDa
Summary Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016703" in other vectors (4)
SKU Description Size Price
MC203971 Preb (untagged) - Mouse prolactin regulatory element binding (Preb), (10ug) 10 ug
$457.00
MG206570 Preb (tGFP-tagged) - Mouse prolactin regulatory element binding (Preb) 10 ug
$657.00
MR206570L3 Lenti ORF clone of Preb (Myc-DDK-tagged) - Mouse prolactin regulatory element binding (Preb) 10 ug
$757.00
MR206570L4 Lenti ORF clone of Preb (mGFP-tagged) - Mouse prolactin regulatory element binding (Preb) 10 ug
$757.00

Plasmid Map for MR206570

Download sequence for

Cloning scheme for MR206570

Circular map for MR206570

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