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Alg2 (NM_019998) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206559
Alg2 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Alg2 or NM_019998"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alg2
Synonyms 1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi; MNCb-5081
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206559 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAAAACCTGTACCGAGCCCGTTCCCGGGTTTACAGCCCATCTGTGCTGTTTCTGCACCCAGACA
TGGGTATAGGCGGAGCCGAGCGCCTAGTGCTGGACGCGGCGCTGGCGCTGCAGGAGTACGGCTGTGATGT
GAAGATATGGACCGCGCACTACGACCCGAACCACTGCTTCATCGAGACCCGCGAGCTCTCGGTGCAATGC
GCAGGGGACTGGCTGCCTCGCAGCCTGGGCTGGGGCGGCCGCGGCGCCGCCATCTGCTCCTACGTGCGCA
TGGTCTTTCTGGCGCTCTACGTGCTGTTTCTCTCCGGCGAGGAGTTCGACGTGGTGGTGTGCGACCAGGT
GTCTGCCTGTATCCCGGTGTTCAAACTGGCCAGACGGCGTAAGAGGGTCCTATTTTACTGTCACTTCCCA
GATCTGCTGCTTACTCAGAGAAATTCAGCTCTGAAGAAGTTCTACAGGGCCCCCATCGACTGGATCGAGG
AATACACCACAGGCATGGCAGACCGCATCTTGGTCAACAGCCAGTACACTGCTTCCGTCTTTAAAGAAAC
CTTCAAGACCCTGTCTCACAGAAATCCTGATGTGCTCTACCCATCTCTGAATATCGGCAGCTTTGACTTG
GCTATTCCTGAAAAGATAGATGACCTCGTCCCCAAGGGGAAGCAATTCCTGTTCCTCTCTATCAACCGAT
ACGAAAGGAAGAAAAATCTGCCCTTGGCACTGAGATCCTTGGTGCAGCTTCGGAATCGGTTACCATCTCA
AGAGTGGGATAAGGTTCATCTCTTCATGGCCGGTGGTTATGACGATAGGATCCCGGAGAACGTGGAGCAC
TATAAGGAGTTGAAGAAAATGGTCCAAGAGTCAGACCTTGAGCGTCATGTGACCTTCCTGCGGTCCTTCT
CGGACAGACAGAAGATCTCACTCCTCCACGGCTGCTTGTGTGTGCTCTACACTCCGAGCAACGAGCACTT
TGGCATCGTCCCTCTGGAGGCCATGTACATGCAGTGCCCAGTCATCGCTGTTAATAACGGTGGGCCCCTG
GAGTCCATTGTCCACAAGGTCACGGGGTTCCTGTGTGAGCCAGACCCAGTGCACTTCTCAGAAGCCATGG
AGAAGTTCATCCACAAACCATCCTTAAAAGCCACGATGGGCCTCGCTGGAAAAGCCAGGGTGGCGGAGAA
GTTTTCAGCTGATGCATTTGCAGACCAGCTGTACCAGTATGTCACGAAGCTGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206559 protein sequence
Red=Cloning site Green=Tags(s)

MAENLYRARSRVYSPSVLFLHPDMGIGGAERLVLDAALALQEYGCDVKIWTAHYDPNHCFIETRELSVQC
AGDWLPRSLGWGGRGAAICSYVRMVFLALYVLFLSGEEFDVVVCDQVSACIPVFKLARRRKRVLFYCHFP
DLLLTQRNSALKKFYRAPIDWIEEYTTGMADRILVNSQYTASVFKETFKTLSHRNPDVLYPSLNIGSFDL
AIPEKIDDLVPKGKQFLFLSINRYERKKNLPLALRSLVQLRNRLPSQEWDKVHLFMAGGYDDRIPENVEH
YKELKKMVQESDLERHVTFLRSFSDRQKISLLHGCLCVLYTPSNEHFGIVPLEAMYMQCPVIAVNNGGPL
ESIVHKVTGFLCEPDPVHFSEAMEKFIHKPSLKATMGLAGKARVAEKFSADAFADQLYQYVTKLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019998
ORF Size 1245 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019998.3
RefSeq Size 3062 bp
RefSeq ORF 1248 bp
Locus ID 56737
UniProt ID Q9DBE8
Cytogenetics 4 B1
MW 47.4 kDa
Summary Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019998" in other vectors (4)
SKU Description Size Price
MC202015 Alg2 (untagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2), (10ug) 10 ug
$457.00
MG206559 Alg2 (tGFP-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2) 10 ug
$657.00
MR206559L3 Lenti ORF clone of Alg2 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2) 10 ug
$757.00
MR206559L4 Lenti ORF clone of Alg2 (mGFP-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2) 10 ug
$757.00

Plasmid Map for MR206559

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206559
Circular map for MR206559

file-alt Citations

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