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Apmap (NM_027977) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206555
Apmap (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310001A20 gene (2310001A20Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Apmap or NM_027977"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apmap
Synonyms 2310001A20Rik; AI314817
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206555 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGAAGCGGACGGGCTGCGGCAGCGTCGGCCCCTGCGGCCGCAGGTCGTCACGGACGATGGCCAGG
TCCCGGAGGTCAAGGAGGGCAGTTCCTTTAGTGGCAGAGTCTTCAGAATGACCTTCTTGATGCTGGCTGT
GTCCCTTGCCATTCCCCTACTTGGAGCCATGATGCTGTTGGAGTCCCCCATAGATCCTCAGAGTTTCAGC
TTCAAAGAACCCCCTTTCATGTTTGGTGTTCTGCATCCAAATACGAAGTTGCGGCAAGCAGAAAGGCTAT
TTGAAAACCAACTTAGTGGACCAGAATCCATAGTAAATATTGGGGATGTGCTGTTTACTGGTACAGCAGA
TGGCCGAGTTGTAAAACTTGAAAATGGAGAAATAGAGACCATCGCTCGGTTCGGTTCAGGCCCTTGCAAA
ACCCGAGATGATGAACCTACCTGTGGGAGACCCCTGGGCATCCGGGCAGGGCCCAATGGGACTCTTTTTG
TGGTTGATGCGTACAAGGGACTGTTCGAAGTAAATCCTCAGAAACGTTCAGTGAAACTGCTGCTATCCTC
TGAGACGCCCATTGAGGGCAAGAAGATGTCCTTTGTGAATGACCTCACTGTTACTCGGGATGGAAGGAAG
ATTTATTTCACGGATTCCAGCAGCAAGTGGCAGAGACGAGACTACCTGCTTCTGGTGATGGAGGCCACTG
ATGATGGGCGCCTGTTAGAGTATGATACTGTGACGAAAGAAGTGAAGGTTTTGTTGGACCAGTTGCAGTT
CCCTAATGGAGTTCAGCTCTCTCCTGAGGAAGACTTTGTCCTAGTGGCAGAGACAACTATGGCCAGGATA
CGAAGAGTCTATGTGTCTGGCCTGATGAAAGGAGGGGCAGATATGTTTGTGGAGAACATGCCTGGATTTC
CTGACAATATCCGGCCTAGCAGCTCTGGCGGATACTGGGTTGCTGCTGCAACCATTCGTGCTAACCCTGG
GTTTTCCATGTTGGATTTCTTATCTGACAAGCCTTTTATTAAGAGAATGATTTTTAAGATGTTCAGTCAG
GAGACAGTGATGAAGTTTGTGCCACGATATAGCCTGGTCCTAGAAGTCAGTGACAGTGGTGCCTTCCGGA
GAAGCCTGCATGATCCTGATGGACAGGTGGTTACCTATGTGAGTGAGGCGCATGAACATGATGGATACCT
GTACCTGGGCTCCTTCAGATCTCCCTTCATCTGCAGACTCAGTCTCCAGTCTATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206555 protein sequence
Red=Cloning site Green=Tags(s)

MSEADGLRQRRPLRPQVVTDDGQVPEVKEGSSFSGRVFRMTFLMLAVSLAIPLLGAMMLLESPIDPQSFS
FKEPPFMFGVLHPNTKLRQAERLFENQLSGPESIVNIGDVLFTGTADGRVVKLENGEIETIARFGSGPCK
TRDDEPTCGRPLGIRAGPNGTLFVVDAYKGLFEVNPQKRSVKLLLSSETPIEGKKMSFVNDLTVTRDGRK
IYFTDSSSKWQRRDYLLLVMEATDDGRLLEYDTVTKEVKVLLDQLQFPNGVQLSPEEDFVLVAETTMARI
RRVYVSGLMKGGADMFVENMPGFPDNIRPSSSGGYWVAAATIRANPGFSMLDFLSDKPFIKRMIFKMFSQ
ETVMKFVPRYSLVLEVSDSGAFRRSLHDPDGQVVTYVSEAHEHDGYLYLGSFRSPFICRLSLQSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027977
ORF Size 1245 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027977.1, NM_027977.2, NP_082253.1
RefSeq Size 2190 bp
RefSeq ORF 1248 bp
Locus ID 71881
UniProt ID Q9D7N9
Cytogenetics 2 G3
MW 46.4 kDa
Summary Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate (By similarity). May play a role in adipocyte differentiation.UniProtKB/Swiss-Prot Function
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"NM_027977" in other vectors (4)
SKU Description Size Price
MC202798 Apmap (untagged) - Mouse RIKEN cDNA 2310001A20 gene (2310001A20Rik), (10ug) 10 ug
$457.00
MG206555 Apmap (tGFP-tagged) - Mouse RIKEN cDNA 2310001A20 gene (2310001A20Rik) 10 ug
$657.00
MR206555L3 Lenti ORF clone of Apmap (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310001A20 gene (2310001A20Rik) 10 ug
$757.00
MR206555L4 Lenti ORF clone of Apmap (mGFP-tagged) - Mouse RIKEN cDNA 2310001A20 gene (2310001A20Rik) 10 ug
$757.00

Plasmid Map for MR206555

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206555
Circular map for MR206555

file-alt Citations

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