Klf15 (NM_023184) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Klf15 |
| Synonyms | 1810013I09Rik; AV048136; AW494632; CKLF; KKLF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_023184 |
| ORF Size | 1245 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_023184.4 |
| RefSeq Size | 2294 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 66277 |
| UniProt ID | Q9EPW2 |
| Cytogenetics | 6 D1 |
| MW | 44.3 kDa |
| Summary | Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_023184" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC205755 | Klf15 (untagged) - Mouse Kruppel-like factor 15 (Klf15), (10ug) | 10 ug |
$457.00
|
|
| MG206548 | Klf15 (tGFP-tagged) - Mouse Kruppel-like factor 15 (Klf15) | 10 ug |
$657.00
|
|
| MR206548L3 | Lenti ORF clone of Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15) | 10 ug |
$757.00
|
|
| MR206548L4 | Lenti ORF clone of Klf15 (mGFP-tagged) - Mouse Kruppel-like factor 15 (Klf15) | 10 ug |
$757.00
|
|
Citations
White adipocytes in subcutaneous fat depots require KLF15 for maintenance in preclinical models
Li, Feldman
J Clin Invest (2024) 134 (13) DOI: 10.1172/JCI172360
Li, Feldman
J Clin Invest (2024) 134 (13) DOI: 10.1172/JCI172360
AI Summary: OriGene’s Klf15 (NM_023184) mouse tagged (Myc-DDK) ORF plasmid (MR206548) was used to study the role of KLF15 in maintaining white adipocyte properties in subcutaneous fat depots.
Snippet: rb1-Mut plasmid was generated from Adrb1-WT plasmid by performing site-directed mutagenesis (E0554S, NEB). Klf15 (NM_023184) mouse tagged (Myc-DDK) ORF was cloned into a pCMV6-Entry vector (MR206548, ORIGENE). All plasmids were purified using the HiPure Plasmid Midiprep Kit (K210004, Thermo Fisher Scientific). Differentiated adipocytes were transfected using Lipofectamine 3000 transfection reagent
Pulsed glucocorticoids enhance dystrophic muscle performance through epigenetic-metabolic reprogramming
Quattrocelli, Zelikovich, Jiang et al
JCI Insight (2019) 4 (24) DOI: 10.1172/jci.insight.132402
Quattrocelli, Zelikovich, Jiang et al
JCI Insight (2019) 4 (24) DOI: 10.1172/jci.insight.132402
Cited by 50
Glucose promotes cell growth by suppressing branched-chain amino acid degradation
Shao, Villet, Zhang et al
Nat Commun (2018) 9 (1), 2935 DOI: 10.1038/s41467-018-05362-7
Shao, Villet, Zhang et al
Nat Commun (2018) 9 (1), 2935 DOI: 10.1038/s41467-018-05362-7
AI Summary: OriGene’s cDNA clones for Glut1 and KLF15 were used in this study to investigate how glucose regulates branched-chain amino acid degradation and promotes cell growth.
Snippet: … The viruses were propagated in 293 cells as described60. The cDNA clones for Glut1 and KLF15 were purchased from Origene. …The cDNA clones for Glut1 and KLF15 were purchased from Origene. Adenovirus harboring β-galactosidase (Ad-LacZ) was used as a control. …
Showing 1-3 of 3 papers.
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