Klf15 (NM_023184) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG206548
Klf15 (tGFP-tagged) - Mouse Kruppel-like factor 15 (Klf15)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Klf15
Synonyms 1810013I09Rik; AV048136; AW494632; CKLF; KKLF
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206548 representing NM_023184
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGACCACCTGCTTCCAGTGGACGAGACCTTCTCGTCACCGAAATGCTCAGTGGGTTACCTAGGGG
ACAGGCTGGCCAGCCGGCAGCCATACCACATGTTGCCCTCGCCCATCTCGGAGGATGACAGCGATGTCTC
CAGCCCCTGCTCTTGTGCCAGCCCTGACTCGCAAGCCTTCTGTTCCTGCTACAGTGCGGGTCCAGGCCCT
GAGGCCCAGGGCAGCATCTTGGATTTCCTCCTGTCCCGGGCCACACTGGGCAGTGGTGGTGGCAGTGGAG
GTATTGGAGATAGCAGTGGCCCTGTGACCTGGGGATCATGGAGGAGAGCCTCTGTGCCTGTGAAGGAGGA
ACATTTCTGCTTCCCTGAATTTCTGTCAGGGGACACTGATGACGTCTCCAGGCCCTTCCAGCCTACCCTG
GAGGAGATTGAAGAATTCCTGGAAGAGAACATGGAGGCTGAGGTCAAGGAGGCCCCAGAGAACGGTAGCA
GGGACCTGGAGACCTGTAGCCAGCTCTCAGCTGGGTCACACCGGAGCCACCTTCATCCAGAGTCTGCTGG
GAGAGAGCGCTGTACCCCACCACCAGGTGGCACGAGTGGGGGTGGTGCCCAAAGTGCAGGTGAGGGGCCA
GCACATGATGGCCCCGTGCCGGTGCTACTGCAGATCCAGCCTGTTGCTGTGAAGCAGGAGGCAGGTACAG
GGCCAGCCTCCCCAGGGCAGGCCCCAGAGAGCGTCAAGGTCGCCCAGCTTCTAGTCAACATCCAGGGGCA
GACCTTTGCACTCCTGCCTCAAGTGGTACCATCCTCCAACTTGAACCTGCCCTCAAAGTTTGTGCGAATT
GCGCCTGTGCCCATTGCCGCCAAACCTATTGGCTCAGGATCCCTAGGGCCCGGCCCTGCTGGCCTCCTTG
TGGGCCAGAAGTTTCCCAAGAACCCAGCAGCAGAACTTCTCAAAATGCACAAATGCACTTTCCCAGGCTG
CAGCAAGATGTACACCAAGAGCAGCCACCTCAAGGCCCACCTGCGTCGGCACACAGGCGAGAAGCCCTTT
GCCTGCACCTGGCCAGGCTGCGGCTGGAGGTTTTCCCGCTCAGATGAGTTGTCAAGGCACCGGCGATCTC
ACTCGGGTGTGAAGCCGTACCAGTGTCCCGTGTGCGAGAAGAAATTCGCGCGGAGTGACCACCTCTCCAA
ACACATCAAAGTGCATCGCTTCCCACGAAGCAGCCGCGCAGTACGCGCCATCAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206548 representing NM_023184
Red=Cloning site Green=Tags(s)

MVDHLLPVDETFSSPKCSVGYLGDRLASRQPYHMLPSPISEDDSDVSSPCSCASPDSQAFCSCYSAGPGP
EAQGSILDFLLSRATLGSGGGSGGIGDSSGPVTWGSWRRASVPVKEEHFCFPEFLSGDTDDVSRPFQPTL
EEIEEFLEENMEAEVKEAPENGSRDLETCSQLSAGSHRSHLHPESAGRERCTPPPGGTSGGGAQSAGEGP
AHDGPVPVLLQIQPVAVKQEAGTGPASPGQAPESVKVAQLLVNIQGQTFALLPQVVPSSNLNLPSKFVRI
APVPIAAKPIGSGSLGPGPAGLLVGQKFPKNPAAELLKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKPF
ACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHIKVHRFPRSSRAVRAIN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023184
ORF Size 1245 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023184.4
RefSeq Size 2294 bp
RefSeq ORF 1248 bp
Locus ID 66277
UniProt ID Q9EPW2
Cytogenetics 6 D1
Summary Transcriptional regulator that binds to the GA element of the CLCNKA promoter (By similarity). Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart. Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. Is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A, GATA4 and MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation (By similarity). Involved in podocyte differentiation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023184" in other vectors (4)
SKU Description Size Price
MC205755 Klf15 (untagged) - Mouse Kruppel-like factor 15 (Klf15), (10ug) 10 ug
$457.00
MR206548 Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15) 10 ug
$289.00 $457.00
MR206548L3 Lenti ORF clone of Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15) 10 ug
$757.00
MR206548L4 Lenti ORF clone of Klf15 (mGFP-tagged) - Mouse Kruppel-like factor 15 (Klf15) 10 ug
$757.00

Plasmid Map for MG206548

Download sequence for

Cloning scheme for MG206548

Circular map for MG206548

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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