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Trmt10c (NM_029092) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206543
Trmt10c (Myc-DDK-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 1 (Rg9mtd1), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trmt10c
Synonyms 1300018J16Rik; D16Ertd454e; Rg9mtd1; Rnmtd1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206543 representing NM_029092
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGTGACTGTCCGTTTCTTAAGACCTTTTGCCAGATGTCTGGTGCCATATACCTTTCATAGGAAGA
GAAGTCATTTATATTCAGGAGTTCTGCAGAGATACATGTCCTCCAAAGCACCTTCTTTGTCTTGTCATAA
TAAGGACAGTGCATCCCCTCCTGAGCAGCTAGAATTGGATGGGTGGAAAGCTACAATGAAGTCGAGCATT
CAAGAAGATGGTGTTTCAGAGGTCTCAGACAAAGATGAAGATTCCCTCGCCTCAACCAGAGAACTAATTG
AGATGTGGAGATTGCTTGGCAAAGAAGTACCAGAACATATTACTGAAGAAGATCTCAAAACCCTTATGGA
ATGTGCCTCTAAATCAGCCAAGAAGAAATACTTACGGTATCTGTATGGGAAGGAAAAGGCGAAAAAAGCT
AAGCAGGTAAAGAAGGAGATGAAAGCGGAGGCCAGGGAAGAAGCAAAAAGGGCCAGGTTGCTAGAGACCA
CCGCAGAAGAACAACAGCAAGACTTCATGTTTCTTCGACTATGGGATCGACAGATCAACATTGCACTGGG
TTGGAAGGGTGTCCAGGCTATGCAGTTTGGACAGCCTTTGGTTTTTGACATGGCCTATGATAATTACATG
AAACCAAGTGAACTGCAGAATACTGTTTCTCAACTTTTAGAAAGTGAAGGATGGAACAGAAGAAATGTTG
ATCCTTTCCACATTTATTTCTGCAATCTTAAAATAGATAGTGCTTATCATAGAGAATTAGTTAAACGTTA
TAGAGAAAAATGGGACAAATTGCTCTTAACAGCAACAGAAAAGTCTCCTGTTGATTTATTTCCAAAGGAC
AGTATTATATATTTAACTGCAGATTCTCCCAATGTTATGACTACCTTCAAGCATGATAAAATTTATATAA
TTGGATCATTTGTTGATAAAAATACACAGACAGGAACATCCTTAGCCAAGGCCAAACGGTTAAACATAGC
AACAGAGTGCCTTCCACTAGATAAATATTTACAATGGGAAATTGGTAACAAAAATCTCACCTTAGATCAG
ATGATCCGAATTTTGCTCTGCCTGAAAAACACTGGTAACTGGGAAGAGGCTCTCAAGTTTGTTCCCAGGA
GAAAGCATACTGGTTATTTAGAGGTTTCTGAGCAATCTCAGGAACTTGTTAGGAAACTGAAGAAGACAAA
GACTTTAAATTCATTTCGAAAAGGCTCCTTAAATGTGCGCACGTGGAAAAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206543 representing NM_029092
Red=Cloning site Green=Tags(s)

MNVTVRFLRPFARCLVPYTFHRKRSHLYSGVLQRYMSSKAPSLSCHNKDSASPPEQLELDGWKATMKSSI
QEDGVSEVSDKDEDSLASTRELIEMWRLLGKEVPEHITEEDLKTLMECASKSAKKKYLRYLYGKEKAKKA
KQVKKEMKAEAREEAKRARLLETTAEEQQQDFMFLRLWDRQINIALGWKGVQAMQFGQPLVFDMAYDNYM
KPSELQNTVSQLLESEGWNRRNVDPFHIYFCNLKIDSAYHRELVKRYREKWDKLLLTATEKSPVDLFPKD
SIIYLTADSPNVMTTFKHDKIYIIGSFVDKNTQTGTSLAKAKRLNIATECLPLDKYLQWEIGNKNLTLDQ
MIRILLCLKNTGNWEEALKFVPRRKHTGYLEVSEQSQELVRKLKKTKTLNSFRKGSLNVRTWKR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029092
ORF Size 1242 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029092.4
RefSeq Size 1846 bp
RefSeq ORF 1245 bp
Locus ID 52575
UniProt ID Q3UFY8
Cytogenetics 16 33.79 cM
MW 48.4 kDa
Summary Mitochondrial tRNA N(1)-methyltransferase involved in mitochondrial tRNA maturation. Component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and MRPP3, which cleaves tRNA molecules in their 5'-ends. Together with HSD17B10/MRPP2, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity. The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)-methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs; TRMT10C/MRPP1 acting as the catalytic N(1)-methyltransferase subunit. The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme. In addition to tRNA N(1)-methyltransferase activity, TRMT10C/MRPP1 also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA.UniProtKB/Swiss-Prot Function
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Documents
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"NM_029092" in other vectors (4)
SKU Description Size Price
MC209897 Trmt10c (untagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 1 (Rg9mtd1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG206543 Trmt10c (tGFP-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 1 (Rg9mtd1) 10 ug
$657.00
MR206543L3 Lenti ORF clone of Trmt10c (Myc-DDK-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 1 (Rg9mtd1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206543L4 Lenti ORF clone of Trmt10c (mGFP-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 1 (Rg9mtd1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206543

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206543
Circular map for MR206543

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