Pten (NM_008960) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pten |
| Synonyms | 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; MMAC; MMAC1; PTENbeta; TEP; TEP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008960 |
| ORF Size | 1209 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008960.2, NP_032986.1 |
| RefSeq Size | 8229 bp |
| RefSeq ORF | 1212 bp |
| Locus ID | 19211 |
| UniProt ID | O08586 |
| Cytogenetics | 19 28.14 cM |
| MW | 47.6 kDa |
| Summary | This gene encodes a phosphatase with dual activity against phospholipids and proteins, and acts as a tumor-suppressor. The protein contains four structural domains, a PIP2-binding domain, a catalytic tensin-type phosphatase domain, a C2 tensin-type domain and a PDZ-binding domain. The protein belongs to the protein tyrosine phosphatase family. Deletion of this gene in mice contribute to tumorigenesis in multiple tissues. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008960" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG206321 | Pten (tGFP-tagged) - Mouse phosphatase and tensin homolog (Pten) | 10 ug |
$886.00
|
|
| MR206321L1 | Lenti ORF clone of Pten (Myc-DDK-tagged) - Mouse phosphatase and tensin homolog (Pten) | 10 ug |
$986.00
|
|
| MR206321L2 | Lenti ORF clone of Pten (mGFP-tagged) - Mouse phosphatase and tensin homolog (Pten) | 10 ug |
$986.00
|
|
| MR206321L3 | Lenti ORF clone of Pten (Myc-DDK-tagged) - Mouse phosphatase and tensin homolog (Pten) | 10 ug |
$986.00
|
|
| MR206321L4 | Lenti ORF clone of Pten (mGFP-tagged) - Mouse phosphatase and tensin homolog (Pten) | 10 ug |
$986.00
|
|
Citations
Cerebellar Ganglioglioma in Childhood: Histopathologic Implications for Management During Long-term Survival: A Case Report
Ohno, Saito, Tamasaki-Kondo et al
Yonago Acta Med (2017) 60 (4), 255-259 DOI: 10.24563/yam.2017.12.008
Ohno, Saito, Tamasaki-Kondo et al
Yonago Acta Med (2017) 60 (4), 255-259 DOI: 10.24563/yam.2017.12.008
Mutation in the FGFR1 tyrosine kinase domain or inactivation of PTEN is associated with acquired resistance to FGFR inhibitors in FGFR1-driven leukemia/lymphomas
Cowell, Qin, Hu et al
Int. J. Cancer (2017)
Cowell, Qin, Hu et al
Int. J. Cancer (2017)
AI Summary: OriGene products, specifically the mouse PTEN ORF clone (Cat# MR206321) and the pLenti-C-Myc-DDK-IRES-tRFP vector (plasmid #PS100080), were used to stably express PTEN in resistant leukemia cells, enabling the researchers to demonstrate that restoring PTEN resensitizes these cells to FGFR inhibitors.
Snippet: Stable expression of human PTEN in resistant cells The wild type, PTEN mouse ORF clone (Cat#MR206321) was purchased from OriGene (www.origene.com) and subsequently subcloned into the pLenti-C-Myc-DDK-IRES-tRFP vector (OriGene, plasmid #PS100080 ... … Stable expression of human PTEN in resistant cells The wild type, PTEN mouse ORF clone (Cat#MR206321) was purchased from OriGene (www.origene.com) and subsequently subcloned into the pLenti-C-Myc-DDK-IRES-tRFP vector (OriGene, plasmid #PS100080 ... …
Showing 1-2 of 2 papers.
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