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Gcnt2 (NM_133219) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206293
Gcnt2 (Myc-DDK-tagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 3
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Gcnt2 or NM_133219"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gcnt2
Synonyms 5330430K10Rik; IGnT; IGnTA; IGnTB; IGnTC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206293 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCTACGCGGGAAAGTTTTCGCTGTCTCTGCGCTCAGCGTGGTGATTTTTGTGGTTTTTTATCACA
GCCAGCTAAGCCTGCCCAACCTCTACCAGCAGCTCAACAGCTCCAGCGAAAGGACGTCTGTCACCATCTG
TGATTACGGCTTGCAAAATCATACGTTCTTCACGACGGGGGACACATCACCACATCCTTTAGAAAGACTC
TCCTGTCCTCAATACCGGATCCAGAGCCACTATATTACCAGTCCCCTTTCGGAAGAAGAGGCTGCCTTTC
CCCTGGCTTACATCATGGTGATCCACAAAGACTTCGATACCTTTGAAAGGCTCTTCAGGGCCATTTACAT
GCCCCAGAACGTCTACTGTGTGCATGTGGATAGTAAGGCGACAGACACCTTCAAAGAAGCGGTGCGGCAG
TTACTAAGCTGTTTCCCCAATGCCTTCCTGGCCTCTAAGGTGGAGCAGGTGGTCTATGGTGGCTTCTCTC
GGCTCCAGGCTGATCTGAACTGCATGAAAGATCTGGTGGCCTCCAAGGTCCCCTGGAAATATGTCCTCAA
CACCTGCGGGCAGGACTTCCCTTTGAAAACCAACAAGGAAATAATTAACCATCTGAAAAGATTTAAGGGG
AAAAACATCACTCCGGGAGTGCTGCCCCCTGCGTACATAGTTGTACGGACTAAATATGTACACCAGGAAC
GCAAAGGCAAAGACGGATATTTTATGCATAAAACAAATATTTTGAAGACTCCACCTCCACACCAACTGAT
CATCTACTTCGGCACAGCCTACGTGGCCCTCACCCGGGACTTTGTCAACTTTATCCTGAATGACGAAAGG
GCCATTGCTCTCTTAGAGTGGTCTAAAGATACCTATAGCCCTGATGAACATTTTTGGGTGACACTCAATA
GGATTCCAGGAGTCCCTGGCTCCATGCCACCAAACGCATCCTGGACGGGTAACCTCAGAGCTGTGAAGTG
GATGGACATGGAAGCGAAGCATGGAGGCTGCCATGGTCACTACGTCCATGGCATTTGTATCTATGGAAAC
GGAGACTTGCAGTGGCTGATTAATTCGCAAAGCCTGTTTGCTAACAAATTTGAACTCAACACATACCCTC
TTACCGTGGAATGCCTGGAACTGAGGCTTCGAGAAAGAACACTCAACCAGAGTGAGATCGCCATACAGCC
GAGCTGGTATTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206293 protein sequence
Red=Cloning site Green=Tags(s)

MSLRGKVFAVSALSVVIFVVFYHSQLSLPNLYQQLNSSSERTSVTICDYGLQNHTFFTTGDTSPHPLERL
SCPQYRIQSHYITSPLSEEEAAFPLAYIMVIHKDFDTFERLFRAIYMPQNVYCVHVDSKATDTFKEAVRQ
LLSCFPNAFLASKVEQVVYGGFSRLQADLNCMKDLVASKVPWKYVLNTCGQDFPLKTNKEIINHLKRFKG
KNITPGVLPPAYIVVRTKYVHQERKGKDGYFMHKTNILKTPPPHQLIIYFGTAYVALTRDFVNFILNDER
AIALLEWSKDTYSPDEHFWVTLNRIPGVPGSMPPNASWTGNLRAVKWMDMEAKHGGCHGHYVHGICIYGN
GDLQWLINSQSLFANKFELNTYPLTVECLELRLRERTLNQSEIAIQPSWYF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133219
ORF Size 1203 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133219.1, NP_573482.1
RefSeq Size 4021 bp
RefSeq ORF 1206 bp
Locus ID 14538
Cytogenetics 13 20.12 cM
MW 46 kDa
Summary Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133219" in other vectors (4)
SKU Description Size Price
MC208534 Gcnt2 (untagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 3, (10ug) 10 ug
$503.00
MG206293 Gcnt2 (tGFP-tagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 3 10 ug
$657.00
MR206293L3 Lenti ORF clone of Gcnt2 (Myc-DDK-tagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 3 10 ug
$757.00
MR206293L4 Lenti ORF clone of Gcnt2 (mGFP-tagged) - Mouse glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (Gcnt2), transcript variant 3 10 ug
$757.00

Plasmid Map for MR206293

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206293
Circular map for MR206293

file-alt Citations

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