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Txnip (BC011212) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206284
Txnip (Myc-DDK-tagged) - Mouse thioredoxin interacting protein (cDNA clone MGC:18703 IMAGE:4193517)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Other products for "Txnip or BC011212"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Txnip
Synonyms THIF, VDUP1, mVDUP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206284 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATGTTCAAGAAGATCAAGTCTTTTGAGGTGGTCTTCAACGACCCCGAGAAAGTGTACGGCAGCG
GGGAGAAGGTGGCCGGACGGGTAATTGTGGAAGTGTGTGAAGTTACCCGAGTCAAAGCTGTCAGGATCCT
GGCTTGCGGCGTGGCCAAGGTCCTGTGGATGCAAGGGTCTCAGCAGTGCAAACAGACTTTGGACTACTTG
CGCTATGAAGACACACTTCTCCTAGAAGAGCAGCCTACAGCAGGTGAGAACGAGATGGTGATCATGAGGC
CTGGAAACAAATATGAGTACAAGTTCGGCTTCGAGCTTCCTAGAGGGCCCCTGGGAACATCCTTTAAAGG
AAAATATGGTTGCGTAGACTACTGGGTGAAGGCTTTTCTCGATCGCCCCAGCCAGCCAACTCAAGAGGCA
AAGAAAAACTTCGAAGTGATGGATCTAGTGGATGTCAATACCCCTGACTTAATGGCACCAGTGTCTGCCA
AAAAGGAGAAGAAAGTTTCCTGCATGTTCATTCCTGATGGACGTGTGTCAGTCTCTGCTCGAATTGACCG
AAAAGGATTCTGTGAAGGTGATGACATCTCCATCCATGCTGACTTTGAGAACACGTGTTCCCGAATCGTG
GTCCCCAAAGCGGCTATTGTGGCCCGACACACTTACCTTGCCAATGGCCAGACCAAAGTGTTCACTCAGA
AGCTGTCCTCGGTCAGAGGCAATCACATTATCTCAGGGACTTGCGCATCGTGGCGTGGCAAGAGCCTCAG
AGTGCAGAAGATCAGACCATCCATCCTGGGCTGCAACATCCTCAAAGTCGAATACTCCTTGCTGATCTAC
GTCAGTGTCCCTGGCTCCAAGAAAGTCATCCTTGATCTGCCCCTAGTGATTGGCAGCAGGTCTGGTCTGA
GCAGCCGGACATCCAGCATGGCCAGCCGGACGAGCTCTGAGATGAGCTGGATAGACCTAAACATCCCAGA
TACCCCAGAAGCTCCTCCTTGCTATATGGACATCATTCCTGAAGATCACAGACTAGAGAGCCCCACCACC
CCTCTGCTGGACGATGTGGACGACTCTCAAGACAGCCCTATCTTTATGTACGCCCCTGAGTTCCAGTTCA
TGCCCCCACCCACTTACACTGAGGTGGATCCGTGCGTCCTTAACAACAACAACAACAACAACAACAACAG
CAACGTGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206284 protein sequence
Red=Cloning site Green=Tags(s)

MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTLDYL
RYEDTLLLEEQPTAGENEMVIMRPGNKYEYKFGFELPRGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQEA
KKNFEVMDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDDISIHADFENTCSRIV
VPKAAIVARHTYLANGQTKVFTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILKVEYSLLIY
VSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWIDLNIPDTPEAPPCYMDIIPEDHRLESPTT
PLLDDVDDSQDSPIFMYAPEFQFMPPPTYTEVDPCVLNNNNNNNNNSNVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC011212
ORF Size 1200 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC011212, AAH11212
RefSeq Size 2799 bp
RefSeq ORF 1202 bp
Locus ID 56338
Cytogenetics 3 41.93 cM
MW 44.7 kDa
Summary May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.UniProtKB/Swiss-Prot Function
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"BC011212" in other vectors (4)
SKU Description Size Price
MC201032 Txnip (untagged) - Mouse thioredoxin interacting protein (cDNA clone MGC:18703 IMAGE:4193517), (10ug) 10 ug
$457.00
MG206284 Txnip (tGFP-tagged) - Mouse thioredoxin interacting protein (cDNA clone MGC:18703 IMAGE:4193517) 10 ug
$657.00
MR206284L3 Lenti ORF clone of Txnip (Myc-DDK-tagged) - Mouse thioredoxin interacting protein (cDNA clone MGC:18703 IMAGE:4193517) 10 ug
$757.00
MR206284L4 Lenti ORF clone of Txnip (mGFP-tagged) - Mouse thioredoxin interacting protein (cDNA clone MGC:18703 IMAGE:4193517) 10 ug
$757.00

Plasmid Map for MR206284

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206284
Circular map for MR206284

file-alt Citations

Deletion of the stress-response protein REDD1 promotes ceramide-induced retinal cell death and JNK activation
Dai, Miller, Toro et al
FASEB J. (2018)
AI Summary: In this study, OriGene's Myc-FLAG-TXNIP expression plasmid (MR206284) was used to investigate the role of TXNIP in retinal cell death and JNK activation, contributing to the elucidation of molecular pathways affected by REDD1 deletion.
Snippet: USA). Plasmid for expression of Myc-FLAG-TXNIP (MR206284) was obtained from Origene (Rockville, MD, USA). Silencer R Origene Technologies (Rockville, MD, USA) DDK (FLAG tag) TA5011-100 A043 - Mouse M Bethyl
Showing 1-1 of 1 papers.
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