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C130026I21Rik (BC007193) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206232
C130026I21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C130026I21 gene (cDNA clone MGC:8305 IMAGE:3593825)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol C130026I21Rik
Synonyms 4930565N07Rik; OTTMUSG00000029174
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206232 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGAGGCTACAATGAACTCAGCATCAGGACAATCCCAGAGGACCAGAATGAAGAGGAGTCAGATG
ACTATCAGCTCATGTTCAAGCACTTCAAAGAAAACAAGGTGGAGATTGCAAGTGCCATCACCAAGCCGTT
TCCTTTCCTCATGAGCCTTCGAGACCGAGACTTCATCTCGGAGCAGAAGTTTCAGGAATATCAAGAAACA
TGTAAGAACCTGGTCCCAGTGGAGAGAGTCGTGTACGATGTCCTCAGCAATGTACAGAAGAATTTTAGCC
GGAATCTTCTGAAAGTGATATTCAGCAAAACACATCTGAAGGCCTACCCCGACTTAAAGGAGACTCTAAA
ACACTTCTTCCTAAATGTTTCTGACAGTCACAGGACTCATCAGAGGATAAATGGAAGAAATGTTGAAGAG
AGGCCCAGACTGCCATCAGTTGTTAGGGAAGCATCCAAAACGAATGATGAACAAGCAGAGGAGATGCTTA
GCCTCCCACAATGCAATGGAGGAGAGGGAAGTAGTTCTTGTGAACAAACACGTGATGAACAAGAGCCCCA
GGATGACCTGCCCTCATCCCTGAGACAAGAAGCAGCAAGAAGAAAAAAACGTCCGAACTGGTCAAATTCC
AAAAGAAGACTGCAGAAAAAAAAGCCCCGTCAAGATGAGATGATGGGAGTGGCCTCACCTGGACATGGAG
TTCAAGAGAAGCTCAAGGCGGTGAGCAGGAGGACTTTGTGGAAAGATGACTCATCTACGAACGTGAAGGA
GGTGACCAAGACACAGAGAGCAAGGATGAGGCGTGCCCAGACATCCAATTCACAAGAGATCAGCAAAGAG
GCATCAAAAACAAGTGGTAGAAAGAGGCCCAGCACAGCACGAAGAACCACACAAGTTCCAGAGAAGACCA
AGAATGACGCTGTGGATTTCTCTCCCACACTCCCTGTGACCTGTGGTAAGGCCAAAGGGACTTTGTTCCA
AGAGAAACTGAAGCAAGGAGCCTCAAAAAAGTGCATTCAGAATGAGGCAGGAGATTGGCTCACTGTAAAG
GAATTTTTAAATGAAGGGAGAAGGGCCACATCAAAAGCCTGGAAGGGCGTTATATGTTGTAACGGGGCGA
CATTAAGACATCTGGAGCAGAAAGGACTTTTGTTCTGTACCTCCAAGAGTAAACCTCAAAAGAAGGGTGC
C

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206232 protein sequence
Red=Cloning site Green=Tags(s)

MAGGYNELSIRTIPEDQNEEESDDYQLMFKHFKENKVEIASAITKPFPFLMSLRDRDFISEQKFQEYQET
CKNLVPVERVVYDVLSNVQKNFSRNLLKVIFSKTHLKAYPDLKETLKHFFLNVSDSHRTHQRINGRNVEE
RPRLPSVVREASKTNDEQAEEMLSLPQCNGGEGSSSCEQTRDEQEPQDDLPSSLRQEAARRKKRPNWSNS
KRRLQKKKPRQDEMMGVASPGHGVQEKLKAVSRRTLWKDDSSTNVKEVTKTQRARMRRAQTSNSQEISKE
ASKTSGRKRPSTARRTTQVPEKTKNDAVDFSPTLPVTCGKAKGTLFQEKLKQGASKKCIQNEAGDWLTVK
EFLNEGRRATSKAWKGVICCNGATLRHLEQKGLLFCTSKSKPQKKGA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC007193
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC007193, AAH07193
RefSeq Size 1684 bp
RefSeq ORF 1193 bp
Locus ID 620078
Cytogenetics 1 C5
MW 45.4 kDa
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"BC007193" in other vectors (4)
SKU Description Size Price
MC200479 C130026I21Rik (untagged) - Mouse RIKEN cDNA C130026I21 gene (cDNA clone MGC:8305 IMAGE:3593825), (10ug) 10 ug
$457.00
MG206232 C130026I21Rik (tGFP-tagged) - Mouse RIKEN cDNA C130026I21 gene (cDNA clone MGC:8305 IMAGE:3593825) 10 ug
$657.00
MR206232L3 Lenti ORF clone of C130026I21Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA C130026I21 gene (cDNA clone MGC:8305 IMAGE:3593825) 10 ug
$757.00
MR206232L4 Lenti ORF clone of C130026I21Rik (mGFP-tagged) - Mouse RIKEN cDNA C130026I21 gene (cDNA clone MGC:8305 IMAGE:3593825) 10 ug
$757.00

Plasmid Map for MR206232

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206232
Circular map for MR206232

file-alt Citations

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