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Ercc8 (NM_028042) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206229
Ercc8 (Myc-DDK-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ercc8 or NM_028042"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ercc8
Synonyms 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik; Ckn1; Csa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206229 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAGCTTTTTGTCCGCTCGACAGTCGGGTTTGGAGGACCCTCTTCGCTTGCGTAGAGCGCAGTCCA
CACGGAGGGTCCTGGGGCTGGAGTTAAACAAAGACAGGGATGTGGAAAGAATCCATGGCAGTGGGGTTAA
CACCCTCGACATTGAGCCCGTTGAAGGAAGATACATGTTGTCAGGTGGCTCCGATGGCGTGGTTGTACTC
TATGACCTTGAGAACGCCAGCAGACAGCCCCATTACACATGTAAAGCAGTGTGTTCCATTGGCAGAAGCC
ATCCCGATGTTCACAAATACAGCGTGGAGACTGTTCAGTGGTATCCTCATGACACTGGCATGTTCACATC
CAGCTCATTTGATAAAACTCTGAAAGTGTGGGATACAAACACATTGCAGGCTGCAGATGTGTTTAATTTT
GAGGAAACAGTTTACAGTCATCACATGTCCCCAGCAGCCACCAAGCACTGTCTGGTAGCAGTTGGAACAA
GAGGACCCAAGGTACAACTTTGTGACTTAAAGTCTGGATCCTGTTCTCACATTCTACAGGGTCACAGACA
GGAAATCTTGGCAGTTTCCTGGTCACCACGCCATGACTATATCTTGGCAACAGCAAGTGCTGACAGTAGA
GTAAAATTATGGGATGTGAGAAGAGCGTCAGGATGCTTGCTTACTCTTGACCAGCATAATGGGAAAAAGT
CACAAGCTGCTGAATCAGCAAACACTGCTCACAATGGGAAAGTTAACGGCTTATGTTTTACAAGTGACGG
CCTTCACCTGCTCACCATTGGCACAGACAACCGAATGCGCCTCTGGAATAGCTCCAGCGGGGACAACACC
CTGGTGAACTATGGGAAGGTGTGTAATGACAGCAGGAAAGGGCTGCAGTTCGCTGTCTCCTGTGGCTGCA
GCTCAGAGTTTGTGTTTGTTCCCCACGGCAGCACCATCGCTGTGTACGCAGTCCACTCGGGAGAGCGGCT
CGCCATGCTCAAGGGACATTACAAAAGCGTCGACTGCTGTGTGTTCCAGCCTAATTTCCAGGAACTTTAC
AGTGGAAGCAGGGACTGCAATATTCTTGCTTGGGTACCACCTTCCTATGAGCCAGTTCCTGATGATGACG
ATGAAGCTCCAGCCAAATCCCAGTTAAACCCAGCATTCGCGGACGCCTGGAGCAGCAGTGACGAGGATGG
G

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206229 protein sequence
Red=Cloning site Green=Tags(s)

MLSFLSARQSGLEDPLRLRRAQSTRRVLGLELNKDRDVERIHGSGVNTLDIEPVEGRYMLSGGSDGVVVL
YDLENASRQPHYTCKAVCSIGRSHPDVHKYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQAADVFNF
EETVYSHHMSPAATKHCLVAVGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRHDYILATASADSR
VKLWDVRRASGCLLTLDQHNGKKSQAAESANTAHNGKVNGLCFTSDGLHLLTIGTDNRMRLWNSSSGDNT
LVNYGKVCNDSRKGLQFAVSCGCSSEFVFVPHGSTIAVYAVHSGERLAMLKGHYKSVDCCVFQPNFQELY
SGSRDCNILAWVPPSYEPVPDDDDEAPAKSQLNPAFADAWSSSDEDG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028042
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028042.1, NM_028042.2, NM_028042.3, NP_082318.2
RefSeq Size 2107 bp
RefSeq ORF 1194 bp
Locus ID 71991
UniProt ID Q8CFD5
Cytogenetics 13 D2.1
MW 43.7 kDa
Summary Involved in transcription-coupled nucleotide excision repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. It is the substrate-recognition component of the CSA complex (DCX(ERCC8) complex) which promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.UniProtKB/Swiss-Prot Function
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"NM_028042" in other vectors (4)
SKU Description Size Price
MC201902 Ercc8 (untagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8), (10ug) 10 ug
$457.00
MG206229 Ercc8 (tGFP-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8) 10 ug
$657.00
MR206229L3 Lenti ORF clone of Ercc8 (Myc-DDK-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8) 10 ug
$757.00
MR206229L4 Lenti ORF clone of Ercc8 (mGFP-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8) 10 ug
$757.00

Plasmid Map for MR206229

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206229
Circular map for MR206229

file-alt Citations

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