OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Atg4a (NM_174875) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR206212
Atg4a (Myc-DDK-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Atg4a or NM_174875"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atg4a
Synonyms AI627006; Apg4a; Atg4al; Autl2; AV169859
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206212 representing NM_174875
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCAGTTATGTCCAAGTATGAAAACCAGATTCTTATTTTCCCTGACTACCTGGAAGAATTCCCGG
ATACAGATGAGCTGGTATGGATTCTGGGGAAGCAGCACCCCCTTAAGACAGAAAAATCTAAGCTGTTGTC
TGATATAAGTGCTCGTCTATGGTTTACATACAGAAGGAAGTTTTCCCCGATTGGGGGAACAGGCCCTTCA
TCTGATGCTGGATGGGGATGTATGCTGCGCTGTGGGCAGATGATGCTGGCTCAAGCCCTCATCTGCAGAC
ACTTGGGAAGGGATTGGAACTGGGAGAGACAAAAAGAACAACCCAAAGAATACCAAAGGATACTGCAGTG
TTTCCTAGATAGAAAAGACTGTTGCTATTCAATCCATCAGATGGCACAAATGGGTGTAGGAGAAGGGAAA
TCAATTGGCGAATGGTTTGGACCAAACACAGTTGCACAGGTGATAAAAAAACTCGCTTTATTTGATGAAT
GGAATTCCTTGGCTGTTTATGTTTCCATGGATAACACAGTGGTCATTGAAGATATCAAGAAAATGTGCTG
TGTTCTTCCTGTGGGTGCTGCTGACCCAGCTGGTGATTTTCTGACTGCATCCAATCAGAGTAGAGACACC
TCTGTCCCGTGCTCAGCCTGGAAACCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCATAAACCAAATCA
ATCCTGTGTATGTTGAAGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCTTTAGGAGGAAA
GCCAAATAACGCCTATTATTTCATAGGATTCTTAGGAGATGAGCTCATTTTTTTGGACCCTCACACAACC
CAGACTTTTGTTGACATTGAAGAGAGTGGACTAGTAGACGACCAGACTTTTCATTGCCTGCAGTCTCCAC
AGCGGATGAGTATCCTGAACTTGGATCCTTCTGTGGCCTTGGGATTTTTCTGCAAAGAAGAGAAAGACTT
TGATAATTGGTGTAGCCTTGTTCAGAAGGAAATTCTAAAGGAGAATTTGAGGATGTTTGAATTGGTTCAG
AAGCACCCATCACACTGGCCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACAGGGGCAGAAT
TCATAGAGTCGACTGAACAACTGGAGGACTTTGAGCTGGAGGAAGATTTTGAAATTCTGAGTGTAGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174875
ORF Size 1188 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174875.5
RefSeq Size 2192 bp
RefSeq ORF 1191 bp
Locus ID 666468
UniProt ID Q8C9S8
Cytogenetics X F1
MW 45.5 kDa
Summary Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_174875" in other vectors (4)
SKU Description Size Price
MC208093 Atg4a (untagged) - Mouse autophagy-related 4A (yeast) (Atg4a), (10ug) 10 ug
$503.00
MG206212 Atg4a (tGFP-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$657.00
MR206212L3 Lenti ORF clone of Atg4a (Myc-DDK-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$757.00
MR206212L4 Lenti ORF clone of Atg4a (mGFP-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$757.00

Plasmid Map for MR206212

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206212
Circular map for MR206212

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products