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Atg4a (NM_174875) Mouse Untagged Clone

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SKU
MC208093
Atg4a (untagged) - Mouse autophagy-related 4A (yeast) (Atg4a), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Atg4a
Synonyms AI627006; Apg4a; Atg4al; Autl2; AV169859
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208093 representing NM_174875
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCAGTTATGTCCAAGTATGAAAACCAGATTCTTATTTTCCCTGACTACCTGGAAGAATTCCCGG
ATACAGATGAGCTGGTATGGATTCTGGGGAAGCAGCACCCCCTTAAGACAGAAAAATCTAAGCTGTTGTC
TGATATAAGTGCTCGTCTATGGTTTACATACAGAAGGAAGTTTTCCCCGATTGGGGGAACAGGCCCTTCA
TCTGATGCTGGATGGGGATGTATGCTGCGCTGTGGGCAGATGATGCTGGCTCAAGCCCTCATCTGCAGAC
ACTTGGGAAGGGATTGGAACTGGGAGAGACAAAAAGAACAACCCAAAGAATACCAAAGGATACTGCAGTG
TTTCCTAGATAGAAAAGACTGTTGCTATTCAATCCATCAGATGGCACAAATGGGTGTAGGAGAAGGGAAA
TCAATTGGCGAATGGTTTGGACCAAACACAGTTGCACAGGTGATAAAAAAACTCGCTTTATTTGATGAAT
GGAATTCCTTGGCTGTTTATGTTTCCATGGATAACACAGTGGTCATTGAAGATATCAAGAAAATGTGCTG
TGTTCTTCCTGTGGGTGCTGCTGACCCAGCTGGTGATTTTCTGACTGCATCCAATCAGAGTAGAGACACC
TCTGTCCCGTGCTCAGCCTGGAAACCCCTGCTGCTCATTGTGCCCCTTCGCCTGGGCATAAACCAAATCA
ATCCTGTGTATGTTGAAGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCTTTAGGAGGAAA
GCCAAATAACGCCTATTATTTCATAGGATTCTTAGGAGATGAGCTCATTTTTTTGGACCCTCACACAACC
CAGACTTTTGTTGACATTGAAGAGAGTGGACTAGTAGACGACCAGACTTTTCATTGCCTGCAGTCTCCAC
AGCGGATGAGTATCCTGAACTTGGATCCTTCTGTGGCCTTGGGATTTTTCTGCAAAGAAGAGAAAGACTT
TGATAATTGGTGTAGCCTTGTTCAGAAGGAAATTCTAAAGGAGAATTTGAGGATGTTTGAATTGGTTCAG
AAGCACCCATCACACTGGCCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACAGGGGCAGAAT
TCATAGAGTCGACTGAACAACTGGAGGACTTTGAGCTGGAGGAAGATTTTGAAATTCTGAGTGTAGGCTA
G

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_174875
Insert Size 1191 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174875.3, NP_777364.3
RefSeq Size 2192 bp
RefSeq ORF 1191 bp
Locus ID 666468
UniProt ID Q8C9S8
Cytogenetics X F1
Summary Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).UniProtKB/Swiss-Prot Function
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"NM_174875" in other vectors (4)
SKU Description Size Price
MG206212 Atg4a (tGFP-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$657.00
MR206212 Atg4a (Myc-DDK-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$289.00 $457.00
MR206212L3 Lenti ORF clone of Atg4a (Myc-DDK-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$757.00
MR206212L4 Lenti ORF clone of Atg4a (mGFP-tagged) - Mouse autophagy-related 4A (yeast) (Atg4a) 10 ug
$757.00

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