Gpr84 (NM_030720) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206206
Gpr84 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 84 (Gpr84)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr84
Synonyms EX33
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206206 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAACAGCTCAGATGCCAACTTCTCCTGCTACCATGAGTCTGTGTTGGGCTATCGATACTTTGCAG
TTATCTGGGGCGTGGCAGTGGCTGTGACAGGCACGGTGGGCAATGTGCTCACTCTGCTGGCCTTGGCCAT
TCGTCCCAAGCTCCGAACCCGCTTCAACCTGCTCATTGCCAACCTCACCCTGGCTGATCTACTCTACTGC
ACGCTCCTGCAGCCTTTCTCCGTGGACACATACCTCCACCTCCATTGGCGTACCGGCGCGGTCTTCTGTA
GAATATTTGGACTCCTCCTCTTTACTTCCAATTCTGTCTCCATCCTCACCCTCTGTCTCATTGCTCTAGG
ACGCTACCTCCTCATTGCCCACCCTAAGCTCTTTCCCCAGGTTTTCAGTGCCAAGGGGATCGTGCTGGCA
CTGGTGGGCAGCTGGGTTGTGGGGGTGACCAGCTTTGCCCCCCTCTGGAATGTTTTTGTCTTGGTGCCAG
TTGTCTGCACCTGCAGCTTTGACCGCATGCGAGGCCGGCCTTACACCACCATCCTCATGGGCATCTACTT
TGTGCTTGGGCTCAGCAGCGTGGGCGTCTTCTACTGCCTCATCCACCGGCAAGTGAAGCGTGCGGCTCGA
GCACTGGACCAATACGGGCTGCATCAGGCCAGCATCCGCTCTCATCAGGTGGCTGGGACACAAGAAGCCA
TGCCTGGCCACTTCCAGGAGCTAGACAGCGGGGTTGCCTCAAGAGGGCCCAGCGAGGGGATTTCATCTGA
GCCAGTCAGTGCTGCGACCACGCAGACCCTGGAAGGTGATTCGTCCGAAGCTGGGGGCCAGGGCATTAGA
AAGGCAGCTCAACAGATCGCAGAGAGAAGCCTTCCAGAAGTGCATCGCAAGCCCCGGGAAACTGCAGGAG
CTCGCAGAGCCACAGATGCCCCATCAGAGTTCGGGAAGGTGACCCGTATGTGCTTCGCAGTGTTCCTCTG
CTTCGCCCTCAGCTACATCCCCTTCCTGTTGCTCAACATTCTGGACGCCAGGGGCCGTGCTCCACGAGTA
GTGCACATGGTGGCTGCCAACCTCACCTGGCTCAACAGCTGCATCAACCCTGTGCTCTATGCAGCCATGA
ACCGCCAGTTTCGCCACGCGTATGGCTCCATCCTGAAACGCGGGCCACAGAGTTTCCGCCGGTTCCAT


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206206 protein sequence
Red=Cloning site Green=Tags(s)

MWNSSDANFSCYHESVLGYRYFAVIWGVAVAVTGTVGNVLTLLALAIRPKLRTRFNLLIANLTLADLLYC
TLLQPFSVDTYLHLHWRTGAVFCRIFGLLLFTSNSVSILTLCLIALGRYLLIAHPKLFPQVFSAKGIVLA
LVGSWVVGVTSFAPLWNVFVLVPVVCTCSFDRMRGRPYTTILMGIYFVLGLSSVGVFYCLIHRQVKRAAR
ALDQYGLHQASIRSHQVAGTQEAMPGHFQELDSGVASRGPSEGISSEPVSAATTQTLEGDSSEAGGQGIR
KAAQQIAERSLPEVHRKPRETAGARRATDAPSEFGKVTRMCFAVFLCFALSYIPFLLLNILDARGRAPRV
VHMVAANLTWLNSCINPVLYAAMNRQFRHAYGSILKRGPQSFRRFH

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_030720
ORF Size 1188 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030720.1, NP_109645.1
RefSeq Size 1611 bp
RefSeq ORF 1191 bp
Locus ID 80910
UniProt ID Q8CIM5
Cytogenetics 15 F3
MW 43.7 kDa
Summary Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030720" in other vectors (4)
SKU Description Size Price
MC204096 Gpr84 (untagged) - Mouse G protein-coupled receptor 84 (Gpr84), (10ug) 10 ug
$686.00
MG206206 Gpr84 (tGFP-tagged) - Mouse G protein-coupled receptor 84 (Gpr84) 10 ug
$886.00
MR206206L3 Lenti ORF clone of Gpr84 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 84 (Gpr84) 10 ug
$986.00
MR206206L4 Lenti ORF clone of Gpr84 (mGFP-tagged) - Mouse G protein-coupled receptor 84 (Gpr84) 10 ug
$986.00

Plasmid Map for MR206206

Download sequence for

Cloning scheme for MR206206

Circular map for MR206206

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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