Rtn4ip1 (NM_130892) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206202
Rtn4ip1 (Myc-DDK-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1), nuclear gene encoding mitochondrial protein
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rtn4ip1
Synonyms D10Ertd690e; NIMP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206202 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGTTTTGAAGACCTGTGTACTTAGAAGAAGTGCTTGTGCCGCCGCCTGCTTCTGGAGAAGGACAG
TTATCCCAAAGCCACCATTTAGAGGCATTAGTACTACGTCTGCGAGGAGCACTGTCATGCCCGCTTGGGT
GATAGATAAATATGGAAAGAATGAAGTCCTTCGATTCACCCAGAACATGATGTTACCTATCATACACTAT
CCAAATGAAGTCATTATCAAAGTCCACGCTGCCAGTGTAAATCCTATAGATGTTAATATGAGAAGTGGTT
ATGGAGCCACAGCCCTAAATATGAAGCGTGATCCCCTGCATATGAAAACCAAAGGAGAAGAGTTTCCTCT
GACTCTGGGTCGGGATGTCTCTGGTGTGGTGATGGAGTGTGGGCTTGATGTGAAGTATTTCCAGCCTGGA
GATGAGGTCTGGGCTGCAGTTCCTCCTTGGAAGCAAGGCACTCTCTCAGAATTTGTTGTGGTCAGTGGGA
ATGAGGTTTCACACAAGCCCAAATCGCTTACTCATACTCAAGCTGCCTCTTTGCCATATGTGGCTCTCAC
GGCCTGGTCTGCCATAAACAAGGTTGGCGGCCTGAGTGACAGGAACTGCAAGGGGAAGCGTGCCTTAATC
TTGGGTGCGTCAGGTGGAGTTGGTACTTTTGCGATACAGGTAATGAAAGCATGGGGGGCTCATGTGACAG
CCGTTTGCTCGAAAGATGCCAGTGAACTTGTGCGGAAGCTTGGAGCAGATGAGGTCATTGATTACACGTT
GGGAAGTGTGGAAGAGCAATTGAAATCTCTAAAACTGTTTGACTTTATCCTTGATAACGTTGGTGGATCT
ACTGAAACATGGGCTCTAAATTTTCTCAAGAAGTGGTCAGGAGCTACCTATGTGACTCTGGTGACTCCTT
TCCTCCTGAACATGGACCGACTGGGTGTTGCTGATGGCATGTTGCAGACAGGAGTCACCGTGGGTACAAA
GGCATTGAAGCATCTCTGGCAAGGAGTCCATTACAGATGGGCCTTCTTCATGGCCAGTGGTCCATATCTA
GATGAAATTGCAGAACTGGTGGATGCAGGAAAGATCCGGCCAGTTATTGAGCGGACATTCCCTTTCTCTG
AAGTCCCAGAAGCCTTCCTGAAGGTGGAGAGAGGCCACGCCCGAGGAAAGACTGTGGTTAATGTAGTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206202 protein sequence
Red=Cloning site Green=Tags(s)

MGVLKTCVLRRSACAAACFWRRTVIPKPPFRGISTTSARSTVMPAWVIDKYGKNEVLRFTQNMMLPIIHY
PNEVIIKVHAASVNPIDVNMRSGYGATALNMKRDPLHMKTKGEEFPLTLGRDVSGVVMECGLDVKYFQPG
DEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLSDRNCKGKRALI
LGASGGVGTFAIQVMKAWGAHVTAVCSKDASELVRKLGADEVIDYTLGSVEEQLKSLKLFDFILDNVGGS
TETWALNFLKKWSGATYVTLVTPFLLNMDRLGVADGMLQTGVTVGTKALKHLWQGVHYRWAFFMASGPYL
DEIAELVDAGKIRPVIERTFPFSEVPEAFLKVERGHARGKTVVNVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130892
ORF Size 1188 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130892.5
RefSeq Size 2981 bp
RefSeq ORF 1191 bp
Locus ID 170728
UniProt ID Q924D0
Cytogenetics 10 23.12 cM
MW 43.4 kDa
Summary Plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve (PubMed:26593267). Appears to be a potent inhibitor of regeneration following spinal cord injury (PubMed:12067236).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_130892" in other vectors (4)
SKU Description Size Price
MC205832 Rtn4ip1 (untagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MG206202 Rtn4ip1 (tGFP-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1) 10 ug
$657.00
MR206202L3 Lenti ORF clone of Rtn4ip1 (Myc-DDK-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206202L4 Lenti ORF clone of Rtn4ip1 (mGFP-tagged) - Mouse reticulon 4 interacting protein 1 (Rtn4ip1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206202

Download sequence for

Cloning scheme for MR206202

Circular map for MR206202

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