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Actr2 (NM_146243) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206161
Actr2 (Myc-DDK-tagged) - Mouse ARP2 actin-related protein 2 homolog (yeast) (Actr2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Actr2 or NM_146243"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Actr2
Synonyms 4921510D23Rik; AA409782; Arp2; D6Ertd746e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206161 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACAGCCAGGGCAGGAAGGTGGTGGTGTGCGACAACGGCACTGGGTTTGTGAAGTGTGGATATGCAG
GCTCTAATTTTCCAGAACACATCTTCCCAGCTTTGGTTGGAAGACCTATTATCAGATCAACCACCAAAGT
GGGAAACATTGAAATCAAGGACCTTATGGTTGGTGATGAGGCAAGTGAGCTGCGTTCCATGTTGGAAGTG
AACTACCCTATGGAGAATGGCATCGTGCGGAATTGGGATGACATGAAGCACCTGTGGGACTACACATTTG
GACCAGAGAAACTCAATATAGACACCAGAAACTGCAAGATTTTACTTACAGAACCTCCCATGAATCCAAC
CAAAAACAGAGAGAAGATTGTAGAGGTAATGTTTGAAACTTACCAGTTTTCTGGTGTGTATGTAGCCATC
CAGGCAGTTCTGACTTTGTATGCTCAAGGTTTACTGACTGGTGTAGTAGTGGACTCTGGAGATGGCGTCA
CTCACATTTGCCCAGTATATGAAGGCTTTTCTCTCCCTCACCTTACAAGGAGGCTGGATATTGCTGGGAG
GGATATTACCAGGTATCTTATCAAGCTGCTGCTGTTGCGAGGATATGCCTTCAACCATTCTGCTGATTTT
GAGACAGTTCGCATGATTAAGGAAAAACTTTGTTATGTGGGTTACAATATTGAGCAGGAGCAGAAGCTGG
CCTTAGAGACCACAGTGTTAGTTGAGTCATATACACTTCCAGATGGACGTATCATTAAGGTTGGAGGAGA
ACGATTTGAAGCACCAGAGGCTTTATTTCAGCCTCATTTGATCAATGTCGAGGGGGTTGGTGTTGCTGAA
CTGCTTTTTAACACAATCCAGGCAGCCGACATTGATACCAGATCTGAATTTTACAAGCACATTGTGCTTT
CTGGAGGTTCTACCATGTATCCTGGCCTGCCATCGAGGTTGGAACGAGAGCTTAAACAGCTTTACTTAGA
ACGAGTTCTGAAAGGAGATGTGGAAAAACTTTCAAAATTTAAGATCCGAATTGAAGACCCACCCCGCAGG
AAGCACATGGTGTTCTTGGGTGGCGCAGTCCTAGCAGACATCATGAAAGACAAAGACAACTTTTGGATGA
CCAGACAAGAGTACCAAGAAAAGGGTGTCCGTGTGCTGGAGAAACTTGGTGTAACTGTTCGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206161 protein sequence
Red=Cloning site Green=Tags(s)

MDSQGRKVVVCDNGTGFVKCGYAGSNFPEHIFPALVGRPIIRSTTKVGNIEIKDLMVGDEASELRSMLEV
NYPMENGIVRNWDDMKHLWDYTFGPEKLNIDTRNCKILLTEPPMNPTKNREKIVEVMFETYQFSGVYVAI
QAVLTLYAQGLLTGVVVDSGDGVTHICPVYEGFSLPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADF
ETVRMIKEKLCYVGYNIEQEQKLALETTVLVESYTLPDGRIIKVGGERFEAPEALFQPHLINVEGVGVAE
LLFNTIQAADIDTRSEFYKHIVLSGGSTMYPGLPSRLERELKQLYLERVLKGDVEKLSKFKIRIEDPPRR
KHMVFLGGAVLADIMKDKDNFWMTRQEYQEKGVRVLEKLGVTVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146243
ORF Size 1182 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146243.1, NP_666355.1
RefSeq Size 3688 bp
RefSeq ORF 1185 bp
Locus ID 66713
UniProt ID P61161
Cytogenetics 11 12.88 cM
MW 44.8 kDa
Summary ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_146243" in other vectors (4)
SKU Description Size Price
MC204224 Actr2 (untagged) - Mouse ARP2 actin-related protein 2 homolog (yeast) (Actr2), (10ug) 10 ug
$457.00
MG206161 Actr2 (tGFP-tagged) - Mouse ARP2 actin-related protein 2 homolog (yeast) (Actr2) 10 ug
$657.00
MR206161L3 Lenti ORF clone of Actr2 (Myc-DDK-tagged) - Mouse ARP2 actin-related protein 2 homolog (yeast) (Actr2) 10 ug
$757.00
MR206161L4 Lenti ORF clone of Actr2 (mGFP-tagged) - Mouse ARP2 actin-related protein 2 homolog (yeast) (Actr2) 10 ug
$757.00

Plasmid Map for MR206161

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206161
Circular map for MR206161

file-alt Citations

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