Kin (NM_025280) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR206100
Kin (Myc-DDK-tagged) - Mouse antigenic determinant of rec-A protein (Kin)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kin
Synonyms Kin17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206100 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAGTCGGATTTTCTGAGCCCCAAAGCCATCGCCAATAGAATTAAGTCCAAAGGGCTCCAGAAGC
TTCGCTGGTACTGCCAGATGTGCCAAAAGCAATGCCGCGACGAGAATGGCTTTAAGTGTCACTGTATGTC
TGAATCTCATCAAAGACAACTGTTGCTGGCTTCAGAAAACCCTCAGCAGTTTATGGATTATTTTTCAGAG
GAATTCCGAAATGACTTTCTGGAACTTCTGAGGCGACGCTTTGGCACTAAAAGGGTCCACAACAACATTG
TCTACAATGAATACATCAGCCACCGAGAGCACATCCACATGAACGCTACTCAGTGGGAGACACTGACCGA
CTTTACCAAGTGGCTGGGCAGAGAGGGCTTGTGTAAAGTGGATGAGACACCGAAAGGCTGGTACATTCAG
TACATAGACAGAGACCCAGAAACCATCCGTCGGCAACTGGAATTAGAAAAAAAGAAGAAGCAAGATCTGG
ACGATGAAGAAAAAACTGCCAAGTTCATTGAGGAGCAGGTGAGAAGAGGCCTGGAAGGGAAAGAGCAGGA
GACACCTGTTTTTACAGAACTAAGCCGAGAAAATGAGGAAGAAAAAGTTACGTTCAATCTGAATAAAGGA
GCGGGTGGCTCAGCGGGAGCTACAACATCCAAGTCAAGCTCTTTGGGACCAAGTGCACTGAAGCTGCTGG
GGAGCGCAGCATCCGGGAAACGGAAAGAGTCTTCACAGAGCTCCGCCCAGCCTGCGAAGAAGAAGAAGTC
CGCCCTGGATGAGATCATGGAGCTCGAAGAGGAAAAGAAAAGGACCGCACGGACAGACGCCTGGTTACAG
CCGGGGATCGTTGTGAAAATTATAACGAAGAAGCTTGGGGAGAAATATCACAAGAAGAAAGGGGTCGTTA
AGGAAGTGATTGACAGGTACACAGCTGTGGTAAAGATGACTGACTCTGGAGACAGGCTGAAACTGGACCA
GACTCATTTAGAGACAGTCATTCCGGCCCCGGGGAAAAGGGTTCTAGTTTTAAATGGAGGCTACAGAGGA
AATGAAGGCACTCTCGAATCCATCAATGAGAAGGCTTTTTCAGCCACGATAGTCATTGAAACTGGACCTT
TGAAAGGACGCAGAGTTGAAGGTATTCAATATGAAGACATATCTAAACTTGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR206100 protein sequence
Red=Cloning site Green=Tags(s)

MGKSDFLSPKAIANRIKSKGLQKLRWYCQMCQKQCRDENGFKCHCMSESHQRQLLLASENPQQFMDYFSE
EFRNDFLELLRRRFGTKRVHNNIVYNEYISHREHIHMNATQWETLTDFTKWLGREGLCKVDETPKGWYIQ
YIDRDPETIRRQLELEKKKKQDLDDEEKTAKFIEEQVRRGLEGKEQETPVFTELSRENEEEKVTFNLNKG
AGGSAGATTSKSSSLGPSALKLLGSAASGKRKESSQSSAQPAKKKKSALDEIMELEEEKKRTARTDAWLQ
PGIVVKIITKKLGEKYHKKKGVVKEVIDRYTAVVKMTDSGDRLKLDQTHLETVIPAPGKRVLVLNGGYRG
NEGTLESINEKAFSATIVIETGPLKGRRVEGIQYEDISKLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025280
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025280.1
RefSeq Size 1379 bp
RefSeq ORF 1176 bp
Locus ID 16588
UniProt ID Q8K339
Cytogenetics 2 A1
MW 44.7 kDa
Summary Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo. Binds via its C-terminal domain to RNA in vitro.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025280" in other vectors (4)
SKU Description Size Price
MC204589 Kin (untagged) - Mouse antigenic determinant of rec-A protein (Kin), (10ug) 10 ug
$457.00
MG206100 Kin (tGFP-tagged) - Mouse antigenic determinant of rec-A protein (Kin) 10 ug
$657.00
MR206100L3 Lenti ORF clone of Kin (Myc-DDK-tagged) - Mouse antigenic determinant of rec-A protein (Kin) 10 ug
$757.00
MR206100L4 Lenti ORF clone of Kin (mGFP-tagged) - Mouse antigenic determinant of rec-A protein (Kin) 10 ug
$757.00

Plasmid Map for MR206100

Download sequence for

Cloning scheme for MR206100

Circular map for MR206100

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