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Pdha1 (NM_008810) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206079
Pdha1 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdha1
Synonyms Pdha-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206079 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAGATGCTTGCCGCTGTATCCCGCGTGTTGGCAGGCTCTGCGCAGAAGCCGGCAAGCCGAGTGC
TGGTTGCTTCCCGTAATTTTGCAAATGATGCTACATTTGAGATTAAGAAATGTGACCTTCATCGGCTAGA
AGAGGGCCCCCCAGTCACCACAGTGCTCACCAGAGAGGATGGGCTCAAGTACTACAGGATGATGCAGACT
GTGCGCCGGATGGAGCTAAAGGCGGATCAGCTGTATAAGCAGAAAATCATTCGTGGTTTCTGTCACTTGT
GTGATGGTCAGGAAGCCTGCTGCGTGGGCCTGGAGGCTGGCATAAACCCTACGGACCACCTCATCACTGC
CTATCGAGCACATGGCTTCACCTTCACTCGGGGCCTGCCTGTGCGAGCAATTCTTGCAGAGCTAACAGGA
CGAAGAGGAGGTTGTGCTAAAGGGAAAGGCGGGTCAATGCACATGTACGCCAAGAACTTCTATGGAGGCA
ACGGCATCGTTGGAGCTCAGGTGCCCCTGGGAGCAGGAATTGCCCTGGCCTGCAAGTACAATGGAAAAGA
TGAGGTCTGTTTGACATTATACGGCGATGGTGCTGCTAATCAGGGTCAGATCTTTGAAGCTTACAATATG
GCAGCACTGTGGAAATTACCTTGCATTTTTATCTGTGAGAACAACCGCTATGGCATGGGGACGTCTGTTG
AGAGAGCAGCAGCCAGCACGGACTACTACAAAAGAGGAGATTTTATTCCTGGACTCAGGGTAGATGGAAT
GGATATCTTGTGCGTCCGAGAGGCAACAAAGTTTGCGGCTGCCTATTGCAGGTCTGGTAAGGGGCCCATC
CTGATGGAGCTCCAGACTTACCGCTACCATGGACACAGCATGAGTGACCCTGGAGTAAGCTACCGCACTC
GAGAAGAAATCCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTCAAGGATAGAATGGTGAACAG
CAATCTTGCAAGTGTTGAAGAATTAAAGGAGATTGATGTGGAAGTGAGGAAAGAAATCGAGGATGCTGCC
CAGTTTGCCACGGCTGATCCTGAGCCCCCGTTGGAGGAACTAGGCTATCACATCTACAGCAGTGATCCTC
CCTTTGAAGTGCGTGGTGCCAACCAGTGGATCAAGTTTAAGTCAGTCAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206079 protein sequence
Red=Cloning site Green=Tags(s)

MRKMLAAVSRVLAGSAQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQT
VRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLPVRAILAELTG
RRGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNM
AALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATKFAAAYCRSGKGPI
LMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAA
QFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008810
ORF Size 1170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008810.3
RefSeq Size 2778 bp
RefSeq ORF 1173 bp
Locus ID 18597
UniProt ID P35486
Cytogenetics X 73.95 cM
MW 43.2 kDa
Summary The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.UniProtKB/Swiss-Prot Function
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"NM_008810" in other vectors (3)
SKU Description Size Price
MC207346 Pdha1 (untagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$457.00
MR206079L3 Lenti ORF clone of Pdha1 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206079L4 Lenti ORF clone of Pdha1 (mGFP-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR206079

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206079
Circular map for MR206079

file-alt Citations

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