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Pdha1 (NM_008810) Mouse Untagged Clone

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SKU
MC207346
Pdha1 (untagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pdha1
Synonyms Pdha-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_008810, the custom clone sequence may differ by one or more nucleotides


ATGAGGAAGATGCTTGCCGCTGTATCCCGCGTGTTGGCAGGCTCTGCGCAGAAGCCGGCAAGCCGAGTGC
TGGTTGCTTCCCGTAATTTTGCAAATGATGCTACATTTGAGATTAAGAAATGTGACCTTCATCGGCTAGA
AGAGGGCCCCCCAGTCACCACAGTGCTCACCAGAGAGGATGGGCTCAAGTACTACAGGATGATGCAGACT
GTGCGCCGGATGGAGCTAAAGGCGGATCAGCTGTATAAGCAGAAAATCATTCGTGGTTTCTGTCACTTGT
GTGATGGTCAGGAAGCCTGCTGCGTGGGCCTGGAGGCTGGCATAAACCCTACGGACCACCTCATCACTGC
CTATCGAGCACATGGCTTCACCTTCACTCGGGGCCTGCCTGTGCGAGCAATTCTTGCAGAGCTAACAGGA
CGAAGAGGAGGTTGTGCTAAAGGGAAAGGCGGGTCAATGCACATGTACGCCAAGAACTTCTATGGAGGCA
ACGGCATCGTTGGAGCTCAGGTGCCCCTGGGAGCAGGAATTGCCCTGGCCTGCAAGTACAATGGAAAAGA
TGAGGTCTGTTTGACATTATACGGCGATGGTGCTGCTAATCAGGGTCAGATCTTTGAAGCTTACAATATG
GCAGCACTGTGGAAATTACCTTGCATTTTTATCTGTGAGAACAACCGCTATGGCATGGGGACGTCTGTTG
AGAGAGCAGCAGCCAGCACGGACTACTACAAAAGAGGAGATTTTATTCCTGGACTCAGGGTAGATGGAAT
GGATATCTTGTGCGTCCGAGAGGCAACAAAGTTTGCGGCTGCCTATTGCAGGTCTGGTAAGGGGCCCATC
CTGATGGAGCTCCAGACTTACCGCTACCATGGACACAGCATGAGTGACCCTGGAGTAAGCTACCGCACTC
GAGAAGAAATCCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTCAAGGATAGAATGGTGAACAG
CAATCTTGCAAGTGTTGAAGAATTAAAGGAGATTGATGTGGAAGTGAGGAAAGAAATCGAGGATGCTGCC
CAGTTTGCCACGGCTGATCCTGAGCCCCCGTTGGAGGAACTAGGCTATCACATCTACAGCAGTGATCCTC
CCTTTGAAGTGCGTGGTGCCAACCAGTGGATCAAGTTTAAGTCAGTCAGTT:AA
Restriction Sites SgfI-MluI |
ACCN NM_008810
Insert Size 1173 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC007142, AAH07142
RefSeq Size 2778 bp
RefSeq ORF 1173 bp
Locus ID 18597
UniProt ID P35486
Cytogenetics X 73.95 cM
Summary The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008810" in other vectors (3)
SKU Description Size Price
MR206079 Pdha1 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $457.00
MR206079L3 Lenti ORF clone of Pdha1 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR206079L4 Lenti ORF clone of Pdha1 (mGFP-tagged) - Mouse pyruvate dehydrogenase E1 alpha 1 (Pdha1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

file-alt Citations

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