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Fbxo30 (BC037691) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR206074
Fbxo30 (Myc-DDK-tagged) - Mouse F-box protein 30 (cDNA clone MGC:47100 IMAGE:3601336)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxo30
Synonyms 1700026A16Rik; MUSA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR206074 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGATGAAGACGATGAGAAGGACTTGTGTTGGAAAAAAGTAGACTTGGGAGACTTGAAGGATGTTA
ATGGTTCACCTTTCAGTCATGCGCCTTCATTCAAGTTTCTTTCTAATTCATGGTATATACCAAAGGAAGA
CAAAGCAGTTGATACATCAGATTTAGAAGTTGCAGAAGATCCCATGGGCCTCCAAGGAATAGATCTGATT
ACAGCAGCATTACTGTTTTGTCTGGGAGATTCTCCAGGTGGGAGAGGTATATCTGATAGCCGCATGACTG
ATGTTTATCACGTTGACTTTGGGACACAGACTTTTTCACTTCCATCTGCAATATTAGCTACAAATACAAT
GGTTGGAGAAATAGCTTCGGCTTCAGCTTGTGATCATGCCAATCCACAGCTTTCAAATCCAAGCCCTTTT
CAGACACTTGGGCTGGACTTAGTTTTGGAATGTGTTGCTAGGTACCAGCCTAAGCAGCGTTCAATGTTTA
CCTTTGTATGCGGACAGCTATTTAGAAGAAAAGAATTTTCTTCCCATTTTAAGAATGTGCATGGTGACAT
TCATGCTGGACTCAATGGATGGATGGAACAGAGGTGTCCTTTAGCTTATTATGGTTGTACCTATTCTCAG
CGTAGATTTTGCCCATCGACACAAGGAGCAAAGATCATACATGATCGCCATTTAAGGTCATTTGGAGTTC
AGCCATGTGTATCTACAGTATTAGAAGAACCTTCTAGAAACTGTGTGTTGGGATTGCGTAGTGACCATCT
AAGTAGTCTCCCTTTTGAGGTACTCCAACATATTGCAGGCTTCCTTGACGGCTTCAGTTTATGCCAGCTT
GCATGTGTATCCCGGCTAATGAGGGACGTTTGTGGCAGTTTACTTCAGTCTCGTGGAATGGTAATCTTGC
AGTGGGGGAAGAAGAAGTATCCGGAAGGAAATTCATCATGGCAGATAAAAGAGAAGGTATGGCGATTCAG
TACTGCATTTTGTTCTGTTAATGACTGGAAATTTGCTGACATCCTGAGCATGGCTGACCACTTGAAGAAC
TGCAGTTACAACGTGATAGAGAAACGGGAGGAAGCCATCCCATTGCCGTGTATGTGTGTGACTCGAGAGC
TTACAAAAGAAGGACGTTCCCTGCGCTCAGTTTTAAAGCCTGTGCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR206074 protein sequence
Red=Cloning site Green=Tags(s)

MPDEDDEKDLCWKKVDLGDLKDVNGSPFSHAPSFKFLSNSWYIPKEDKAVDTSDLEVAEDPMGLQGIDLI
TAALLFCLGDSPGGRGISDSRMTDVYHVDFGTQTFSLPSAILATNTMVGEIASASACDHANPQLSNPSPF
QTLGLDLVLECVARYQPKQRSMFTFVCGQLFRRKEFSSHFKNVHGDIHAGLNGWMEQRCPLAYYGCTYSQ
RRFCPSTQGAKIIHDRHLRSFGVQPCVSTVLEEPSRNCVLGLRSDHLSSLPFEVLQHIAGFLDGFSLCQL
ACVSRLMRDVCGSLLQSRGMVILQWGKKKYPEGNSSWQIKEKVWRFSTAFCSVNDWKFADILSMADHLKN
CSYNVIEKREEAIPLPCMCVTRELTKEGRSLRSVLKPVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC037691
ORF Size 1167 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037691, AAH37691
RefSeq Size 2119 bp
RefSeq ORF 1169 bp
Locus ID 71865
Cytogenetics 10 A1
MW 43.5 kDa
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Required for muscle atrophy following denervation.UniProtKB/Swiss-Prot Function
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"BC037691" in other vectors (4)
SKU Description Size Price
MC217880 Fbxo30 (untagged) - Mouse F-box protein 30 (cDNA clone MGC:47100 IMAGE:3601336), (10ug) 10 ug
$503.00
MG206074 Fbxo30 (tGFP-tagged) - Mouse F-box protein 30 (cDNA clone MGC:47100 IMAGE:3601336) 10 ug
$657.00
MR206074L3 Lenti ORF clone of Fbxo30 (Myc-DDK-tagged) - Mouse F-box protein 30 (cDNA clone MGC:47100 IMAGE:3601336) 10 ug
$757.00
MR206074L4 Lenti ORF clone of Fbxo30 (mGFP-tagged) - Mouse F-box protein 30 (cDNA clone MGC:47100 IMAGE:3601336) 10 ug
$757.00

Plasmid Map for MR206074

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR206074
Circular map for MR206074

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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