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Jaml (NM_001005421) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205923
Amica1 (Myc-DDK-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Jaml or NM_001005421"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Jaml
Synonyms AMICA; Amica1; Crea7; Gm638
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205923 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTTGCCTCCTGAAACTGATTGTGATTCCAGTAATCCTGGCCCCTGTAGGTTATCCACAGGGCCTGC
CAGGCTTGACCGTTTCCTCCCCTCAGCTGAGAGTGCATGTGGGTGAATCAGTCTTGATGGGATGTGTTGT
CCAGCGCACAGAAGAGAAACACGTGGACAGAGTGGATTGGCTCTTCTCGAAAGATAAAGATGATGCGAGT
GAATATGTGCTGTTCTACTATTCCAACCTTAGCGTGCCTACGGGGCGCTTCCAGAACCGGTCACATTTGG
TGGGGGACACCTTCCATAATGATGGTTCTCTCCTGCTCCAAGATGTTCAGAAGGCCGATGAGGGAATCTA
CACCTGTGAAATCCGCCTCAAAAATGAGAGCATGGTGATGAAAAAGCCCGTGGAACTGTGGGTGCTACCA
GAGGAACCTAAAGATCTCAGAGTCCGAGTAGGTGATACAACTCAGATGAGATGTTCTATCCAGAGCACAG
AAGAGAAACGGGTGACCAAAGTAAACTGGATGTTTTCTTCAGGGAGCCATACTGAGGAGGAGACAGTCTT
GAGCTATGACTCCAACATGCGTAGTGGAAAATTCCAGAGCCTGGGCCGCTTCCGCAACCGTGTAGACCTG
ACAGGTGACATCTCCCGCAATGATGGCTCAATCAAACTTCAAACAGTGAAGGAGTCTGACCGAGGAATCT
ACACTTGCAGCATCTACGTGGGAAAGCTGGAGTCCAGGAAAACCATTGTGCTGCATGTGGTCCAGGACGA
ATTTCAAAGGACAATTTCACCAACTCCTCCAACTGATAAGGGTCAGCAGGGCATCCTGAATGGAAATCAG
CTGGTGATCATTGTGGGGATCGTCTGTGCCACCTTCCTGCTGCTTCCGGTTTTGATATTAATTGTGAAGA
AAGCCAAGTGGAATAAGAGCTCAGTAAGTTCTATGGCTTCTGTGAAGAGCCTGGAGAACAAAGAGAAGAT
TAATCCAGAGAAGCATATCTACTCCTCCATAACTACGTGGGAGACGACAGAGAGAGGAATAAGTGGAGAG
TCAGAGGGCACCTACATGACCATGAATCCAGTTTGGCCTTCTTCCCCCAAAGCATCCAGTTTGGTCCGCT
CTTCAGTCAGATCCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205923 protein sequence
Red=Cloning site Green=Tags(s)

MLCLLKLIVIPVILAPVGYPQGLPGLTVSSPQLRVHVGESVLMGCVVQRTEEKHVDRVDWLFSKDKDDAS
EYVLFYYSNLSVPTGRFQNRSHLVGDTFHNDGSLLLQDVQKADEGIYTCEIRLKNESMVMKKPVELWVLP
EEPKDLRVRVGDTTQMRCSIQSTEEKRVTKVNWMFSSGSHTEEETVLSYDSNMRSGKFQSLGRFRNRVDL
TGDISRNDGSIKLQTVKESDRGIYTCSIYVGKLESRKTIVLHVVQDEFQRTISPTPPTDKGQQGILNGNQ
LVIIVGIVCATFLLLPVLILIVKKAKWNKSSVSSMASVKSLENKEKINPEKHIYSSITTWETTERGISGE
SEGTYMTMNPVWPSSPKASSLVRSSVRSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005421
ORF Size 1137 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005421.1
RefSeq Size 2472 bp
RefSeq ORF 1140 bp
Locus ID 270152
UniProt ID Q80UL9
Cytogenetics 9 A5.2
MW 42.6 kDa
Summary Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001005421" in other vectors (4)
SKU Description Size Price
MC201873 Amica1 (untagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1), (10ug) 10 ug
$457.00
MG205923 Amica1 (tGFP-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1) 10 ug
$489.00 $657.00
MR205923L3 Lenti ORF clone of Amica1 (Myc-DDK-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1) 10 ug
$757.00
MR205923L4 Lenti ORF clone of Amica1 (mGFP-tagged) - Mouse adhesion molecule, interacts with CXADR antigen 1 (Amica1) 10 ug
$757.00

Plasmid Map for MR205923

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205923
Circular map for MR205923

file-alt Citations

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