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Ring1 (NM_009066) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205874
Ring1 (Myc-DDK-tagged) - Mouse ring finger protein 1 (Ring1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ring1
Synonyms Ring1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205874 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGTACAGAGATTGCGGTTTCGCCTCGGTCACTGCATTCTGAGCTCATGTGTCCCATCTGCCTGG
ACATGCTGAAGAACACAATGACCACCAAGGAGTGCCTGCATAGGCTCTGCTCGGACTGCATCGTCACCGC
CCTGCGGAGCGGGAACAAGGAGTGCCCTACCTGCCGGAAAAAGCTGGTATCCAAGCGGTCCCTACGGCCG
GACCCCAACTTCGACGCCCTGATCTCCAAAATCTACCCTAGCCGGGAGGAATATGAGGCCCATCAGGACC
GGGTGCTCATCCGCCTCAGCCGCCTGCACAACCAGCAGGCGCTGAGCTCCAGTATCGAGGAAGGGCTCCG
GATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATGCCTGGATCTGATCAGACCGCCACAATG
AGTGGGGGGGAAGGAGAACCTGGGGAGGGAGAAGGGGATGGAGAGGATGTAAGCTCTGACTCCGCCCCAG
ACTCTGCTCCAGGCCCTGCTCCCAAGCGACCCCGCGGAGCGGGTGCAGGGGCCAGCAGTGTAGGGACAGG
GGGTGGGGCTGCTGGTGGGGCTTGCGGGGGTGCCGGTTCTGAAGACTCTGGTGACCGGGGCGGCACCCTG
GGAGGGGGAACCCTGGGGCCCCCAAGCCCTCCTGGGGCCCCCAGCCCTCCAGAGCCCGGTGGAGAGATTG
AGCTTGTGTTCCGGCCCCATCCCCTGCTTGTGGAGAAAGGAGAGTACTGCCAGACTCGGTACGTGAAGAC
TACTGGGAATGCCACAGTGGATCATCTCTCCAAGTACCTGGCCCTGCGCATTGCCCTGGAGAGGAGGCAG
CAGCAGGAAACCGCAGAGCCCGGAGGGCCTGGTGGGGGTGCTTCGGACACAGGCGGACCTGATGGGGGTG
GTGGGGAGAGAGGCGTTGCCGGAGGAGGCGAAGGTCCTGAGGAGCCTGCGCTCCCCAGCCTGGAAGGTGT
CAGCGAAAAGCAGTACACCATCTACATCGCTCCTGGGGGCGGAGCGTTCACGACGCTGAATGGATCACTG
ACCTTGGAGCTTGTAAACGAGAAGTTCTGGAAGGTGTCCAGGCCCTTAGAGCTCTGCTATGCCCCCACGA
AGGACCCAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205874 protein sequence
Red=Cloning site Green=Tags(s)

MDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRLCSDCIVTALRSGNKECPTCRKKLVSKRSLRP
DPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPMPGSDQTATM
SGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGAGAGASSVGTGGGAAGGACGGAGSEDSGDRGGTL
GGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIALERRQ
QQETAEPGGPGGGASDTGGPDGGGGERGVAGGGEGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSL
TLELVNEKFWKVSRPLELCYAPTKDPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009066
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009066.2, NP_033092.2
RefSeq Size 2048 bp
RefSeq ORF 1221 bp
Locus ID 19763
UniProt ID O35730
Cytogenetics 17 B1
MW 39.3 kDa
Summary Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009066" in other vectors (4)
SKU Description Size Price
MC207379 Ring1 (untagged) - Mouse ring finger protein 1 (Ring1), (10ug) 10 ug
$503.00
MG205874 Ring1 (tGFP-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$657.00
MR205874L3 Lenti ORF clone of Ring1 (Myc-DDK-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$757.00
MR205874L4 Lenti ORF clone of Ring1 (mGFP-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$757.00

Plasmid Map for MR205874

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205874
Circular map for MR205874

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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