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Ring1 (NM_009066) Mouse Untagged Clone

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SKU
MC207379
Ring1 (untagged) - Mouse ring finger protein 1 (Ring1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ring1
Synonyms Ring1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207379 representing NM_009066
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGACGCCGGCGAATGCACAGAATGCCAGCAAAACGTGGGAACTGAGTCTCTATGAGCTGCACCGGA
CCCCGCAGGAAGCCATCATGGATGGTACAGAGATTGCGGTTTCGCCTCGGTCACTGCATTCTGAGCTCAT
GTGTCCCATCTGCCTGGACATGCTGAAGAACACAATGACCACCAAGGAGTGCCTGCATAGGTTCTGCTCG
GACTGCATCGTCACCGCCCTGCGGAGCGGGAACAAGGAGTGCCCTACCTGCCGGAAAAAGCTGGTATCCA
AGCGGTCCCTACGGCCGGACCCCAACTTCGACGCCCTGATCTCCAAAATCTACCCTAGCCGGGAGGAATA
CGAGGCCCATCAGGACCGGGTGCTCATCCGCCTCAGCCGCCTGCACAACCAGCAGGCGCTGAGCTCCAGT
ATCGAGGAAGGGCTCCGGATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATGCCTGGATCTG
ATCAGACCGCCACAATGAGTGGGGGGGAAGGAGAACCTGGGGAGGGAGAAGGGGATGGAGAGGATGTAAG
CTCTGACTCCGCCCCAGACTCTGCTCCAGGCCCTGCTCCCAAGCGACCCCGCGGAGCGGGTGCAGGGGCC
AGCAGTGTAGGGACAGGGGGTGGGGCTGCTGGTGGGGCTTGCGGGGGTGCCGGTTCTGAAGACTCTGGTG
ACCGGGGCGGCACCCTGGGAGGGGGAACCCTGGGGCCCCCAAGCCCTCCTGGGGCCCCCAGCCCTCCAGA
GCCCGGTGGAGAGATTGAGCTTGTGTTCCGGCCCCATCCCCTGCTTGTGGAGAAAGGAGAGTACTGCCAG
ACTCGGTACGTGAAGACTACTGGGAATGCCACAGTGGATCATCTCTCCAAGTACCTGGCCCTGCGCATTG
CCCTGGAGAGGAGGCAGCAGCAGGAAACCACAGAGCCCGGAGGGCCTGGTGGGGGTGCTTCGGACACAGG
CGGACCTGATGGGGGTGGTGGGGAGAGAGGCGTTGCCGGAGGAGGCGAAGGTCCTGAGGAGCCTGCGCTG
CCCAGCCTGGAAGGTGTCAGCGAAAAGCAGTACACCATCTACATCGCTCCTGGGGGCGGAGCGTTCACGA
CGCTGAATGGATCACTGACCTTGGAGCTTGTAAACGAGAAGTTCTGGAAGGTGTCCAGGCCCTTAGAGCT
CTGCTATGCCCCCACGAAGGACCCAAAATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_009066
Insert Size 1221 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009066.3, NP_033092.3
RefSeq Size 2048 bp
RefSeq ORF 1221 bp
Locus ID 19763
UniProt ID O35730
Cytogenetics 17 B1
Summary Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009066" in other vectors (4)
SKU Description Size Price
MG205874 Ring1 (tGFP-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$657.00
MR205874 Ring1 (Myc-DDK-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$289.00 $457.00
MR205874L3 Lenti ORF clone of Ring1 (Myc-DDK-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$757.00
MR205874L4 Lenti ORF clone of Ring1 (mGFP-tagged) - Mouse ring finger protein 1 (Ring1) 10 ug
$757.00

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