Serpinb5 (NM_009257) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205822
Serpinb5 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 5 (Serpinb5)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Serpinb5
Synonyms 1110036M19Rik; AI462524; AI646751; Maspin; ovalbumin; PI-5; Spi7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205822 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCCCTGAGACTGGCAAATTCAGCTTTTGCTGTTGACTTGTTCAAACAACTATGTGAAAGGGACC
CAGCAGGAAACATTCTCTTCTCTCCAATATGCCTCTCTACTTCTCTGTCCCTTGCGCAAGTGGGCACCAA
AGGCGACACAGCAAATGAAATTGGACAGGTCCTTCATTTTGAGAATGTCAAAGATGTACCCTTTGGGTTT
CAAACAGTCACTTCTGATGTTAATAAGCTCAGTTCTTTTTACTCTTTGAAACTTGTCAAGCGACTCTACA
TAGACAAATCCCTGAACCCTTCTACAGAATTTATCAGTTCTACCAAAAGACCATATGCAAAAGAATTGGA
AACTGTTGACTTCAAAGACAAACTGGAAGAAACGAAAGGTCAAATTAACAGCTCCATTAAGGAGCTCACA
GATGGCCACTTTGAGGACATTTTGTCAGAGAACAGTATAAGTGACCAGACCAAAATCCTTGTGGTTAATG
CTGCCTACTTTGTTGGAAAGTGGATGAAGAAATTTCCGGAATCAGAAACAAAAGAATGTCCTTTCAGAAT
CAGCAAGACAGACACCAAACCCGTACAAATGATGAATCTTGAGGCCACTTTCTGCTTGGGTAACATTGAT
GACATCAGCTGTAAGATCATAGAACTTCCTTTCCAGAATAAGCATCTGAGTATGCTCATTGTGCTCCCCA
AGGACGTGGAGGATGAGTCCACAGGCCTGGAGAAGATTGAACAGCAACTCAACCCAGAAACATTGTTACA
GTGGACCAACCCCAGTACCATGGCCAATGCCAAAGTCAAACTTTCCCTCCCAAAGTTTAAGGTAGAAAAG
ATGATTGATCCCAAGGCTAGTCTGGAAAGCCTAGGGCTGAAAAGTCTCTTCAATGAAAGTACATCGGATT
TCTCTGGAATGTCAGAGACCAAGGGAGTGTCCCTGTCAAATGTGATTCATAGAGTATGCCTAGAAATAAC
CGAAGATGGTGGTGAGTCCATCGAGGTGCCAGGGTCCCGGATCTTACAGCACAAGGATGAATTCAATGCT
GACCATCCATTTATTTATATCATTAGACACAACAAAACTCGAAACATCATTTTCTTTGGCAAATTCTGTT
CTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205822 protein sequence
Red=Cloning site Green=Tags(s)

MDALRLANSAFAVDLFKQLCERDPAGNILFSPICLSTSLSLAQVGTKGDTANEIGQVLHFENVKDVPFGF
QTVTSDVNKLSSFYSLKLVKRLYIDKSLNPSTEFISSTKRPYAKELETVDFKDKLEETKGQINSSIKELT
DGHFEDILSENSISDQTKILVVNAAYFVGKWMKKFPESETKECPFRISKTDTKPVQMMNLEATFCLGNID
DISCKIIELPFQNKHLSMLIVLPKDVEDESTGLEKIEQQLNPETLLQWTNPSTMANAKVKLSLPKFKVEK
MIDPKASLESLGLKSLFNESTSDFSGMSETKGVSLSNVIHRVCLEITEDGGESIEVPGSRILQHKDEFNA
DHPFIYIIRHNKTRNIIFFGKFCSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009257
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009257.2
RefSeq Size 2565 bp
RefSeq ORF 1128 bp
Locus ID 20724
UniProt ID P70124
Cytogenetics 1 E2.1
MW 42.1 kDa
Summary Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009257" in other vectors (4)
SKU Description Size Price
MC207406 Serpinb5 (untagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 5 (Serpinb5), (10ug) 10 ug
$503.00
MG205822 Serpinb5 (tGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 5 (Serpinb5) 10 ug
$657.00
MR205822L3 Lenti ORF clone of Serpinb5 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 5 (Serpinb5) 10 ug
$757.00
MR205822L4 Lenti ORF clone of Serpinb5 (mGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 5 (Serpinb5) 10 ug
$757.00

Plasmid Map for MR205822

Download sequence for

Cloning scheme for MR205822

Circular map for MR205822

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