Il2rg (NM_013563) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR205689
Il2rg (Myc-DDK-tagged) - Mouse interleukin 2 receptor, gamma chain (Il2rg)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Il2rg
Synonyms CD132; gamm; gamma(; gamma(c); gc; p64; gc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205689 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAAACTATTATTGTCACCTAGATCCTTCTTAGTCCTTCAGCTGCTCCTGCTGAGGGCAGGGTGGA
GCTCCAAGGTCCTCATGTCCAGTGCGAATGAAGACATCAAAGCTGATTTGATCCTGACTTCTACAGCCCC
TGAACACCTCAGTGCTCCTACTCTGCCCCTTCCAGAGGTTCAGTGCTTTGTGTTCAACATAGAGTACATG
AATTGCACTTGGAATAGCAGTTCTGAGCCTCAGGCAACCAACCTCACGCTGCACTATAGGTACAAGGTAT
CTGATAATAATACATTCCAGGAGTGCAGTCACTATTTGTTCTCCAAAGAGATTACTTCTGGCTGTCAGAT
ACAAAAAGAAGATATCCAGCTCTACCAGACATTTGTTGTCCAGCTCCAGGACCCCCAGAAACCCCAGAGG
CGAGCTGTACAGAAGCTAAACCTACAGAATCTTGTGATCCCACGGGCTCCAGAAAATCTAACACTCAGCA
ATCTGAGTGAATCCCAGCTAGAGCTGAGATGGAAAAGCAGACATATTAAAGAACGCTGTTTACAATACTT
GGTGCAGTACCGGAGCAACAGAGATCGAAGCTGGACGGAACTAATAGTGAATCATGAACCTAGATTCTCC
CTGCCTAGTGTGGATGAGCTGAAACGGTACACATTTCGGGTTCGGAGCCGCTATAACCCAATCTGTGGAA
GTTCTCAACAGTGGAGTAAATGGAGCCAGCCTGTCCACTGGGGGAGTCATACTGTAGAGGAGAATCCTTC
CTTGTTTGCACTGGAAGCTGTGCTTATCCCTGTTGGCACCATGGGGTTGATTATTACCCTGATCTTTGTG
TACTGTTGGTTGGAACGAATGCCTCCAATTCCCCCCATCAAGAATCTAGAGGATCTGGTTACTGAATACC
AAGGGAACTTTTCGGCCTGGAGTGGTGTGTCTAAAGGGCTGACTGAGAGTCTGCAGCCAGACTACAGTGA
ACGGTTCTGCCACGTCAGCGAGATTCCCCCCAAAGGAGGGGCCCTAGGAGAGGGGCCTGGAGGTTCTCCT
TGCAGCCTGCATAGCCCTTACTGGCCTCCCCCATGTTATTCTCTGAAGCCGGAAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR205689 protein sequence
Red=Cloning site Green=Tags(s)

MLKLLLSPRSFLVLQLLLLRAGWSSKVLMSSANEDIKADLILTSTAPEHLSAPTLPLPEVQCFVFNIEYM
NCTWNSSSEPQATNLTLHYRYKVSDNNTFQECSHYLFSKEITSGCQIQKEDIQLYQTFVVQLQDPQKPQR
RAVQKLNLQNLVIPRAPENLTLSNLSESQLELRWKSRHIKERCLQYLVQYRSNRDRSWTELIVNHEPRFS
LPSVDELKRYTFRVRSRYNPICGSSQQWSKWSQPVHWGSHTVEENPSLFALEAVLIPVGTMGLIITLIFV
YCWLERMPPIPPIKNLEDLVTEYQGNFSAWSGVSKGLTESLQPDYSERFCHVSEIPPKGGALGEGPGGSP
CSLHSPYWPPPCYSLKPEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013563
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013563.4
RefSeq Size 1612 bp
RefSeq ORF 1110 bp
Locus ID 16186
UniProt ID P34902
Cytogenetics X 43.9 cM
MW 42.2 kDa
Summary This gene encodes a transmembrane protein that is a common subunit of several interleukin receptor complexes. These receptors are comprised of alpha and beta subunits in addition to this gamma subunit. Signalling through this pathway in important in immune cell differentiation and function. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2015
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Documents
Resources
"NM_013563" in other vectors (4)
SKU Description Size Price
MC204067 Il2rg (untagged) - Mouse interleukin 2 receptor, gamma chain (Il2rg), (10ug) 10 ug
$457.00
MG205689 Il2rg (tGFP-tagged) - Mouse interleukin 2 receptor, gamma chain (Il2rg) 10 ug
$657.00
MR205689L3 Lenti ORF clone of Il2rg (Myc-DDK-tagged) - Mouse interleukin 2 receptor, gamma chain (Il2rg) 10 ug
$757.00
MR205689L4 Lenti ORF clone of Il2rg (mGFP-tagged) - Mouse interleukin 2 receptor, gamma chain (Il2rg) 10 ug
$757.00

Plasmid Map for MR205689

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Cloning scheme for MR205689

Circular map for MR205689

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