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Ddx25 (NM_013932) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR205683
Ddx25 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ddx25
Synonyms AW047046; GRTH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR205683 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGATTTAACCGACCATCCAAAATCCAAGAGATGGCCCTCCCCATGATGCTAGCACACCCTCCCCAGA
ACCTCATAGCACAGAGCCAGTCCGGCACAGGAAAAACTGCTGCCTTTGTCTTAGCAATGCTAAGCCGAGT
GAATGCCTTGGAACTGTTTCCACAGTGCCTCTGCCTGGCTCCTACCTATGAACTGGCTCTGCAGACCGGT
CGTGTGGTTGAAAGGATGGGCAAGTTCTGCGTGGATGTTGAAGTGATGTATGCCATTCGAGGAAACCGAA
TTCCAAGGGGCACCGAAGTCACCAAACAGATTATTATTGGTACTCCTGGGACTGTCCTAGATTGGTGTTT
CAAGCGAAAACTGATTGATTTGACAAAGATCCGTGTGTTTGTCCTGGATGAAGCAGATGTGATGATTGAC
ACCCAAGGATTCTCCGATCAGAGCATCCGTATCCAAAGAGCTTTACCCTCTGAATGCCAGATGCTCCTCT
TTTCGGCAACCTTTGAGGACTCTGTGTGGCAGTTTGCTGAACGAATCATTCCTGACCCCAATGTTATCAA
GTTGCGAAAAGAGGAACTGACCCTGAACAACATCCGGCAGTATTACGTGTTGTGTGAGAACAGGAAAGGC
AAATACCAAGCCCTCTGTAACATCTACGGTGGCATCACCATTGGCCAAGCCATTATCTTCTGCCAGACAC
GTCGAAATGCCAAGTGGCTGACTGTGGAGATGATGCAGGATGGCCACCAGGTGTCCCTGCTAAGTGGGGA
ACTCACAGTGGAGCAGCGAGCTTCCATCATTCAGAGATTCCGAGATGGCAAAGAGAAGGTTCTCATAACG
ACCAATGTCTGTGCTCGAGGGATTGATGTGAAGCAGGTCACCATCGTCGTGAACTTTGACCTCCCTGTAA
ACCAATCAGAAGAGCCAGACTATGAGACCTACCTCCACCGTATAGGGCGGACGGGACGCTTTGGGAAAAA
AGGTCTCGCCTTCAACATGATTGAAGTGGATAAGCTGCCCCTGCTTATGAAAATACAAGACCCCTTCAAT
AGCAATATTAAGCAGCTTGACCCAGAAGACATGGATGAGATTGAGAAGATCGAATAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR205683 protein sequence
Red=Cloning site Green=Tags(s)

MGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLSRVNALELFPQCLCLAPTYELALQTG
RVVERMGKFCVDVEVMYAIRGNRIPRGTEVTKQIIIGTPGTVLDWCFKRKLIDLTKIRVFVLDEADVMID
TQGFSDQSIRIQRALPSECQMLLFSATFEDSVWQFAERIIPDPNVIKLRKEELTLNNIRQYYVLCENRKG
KYQALCNIYGGITIGQAIIFCQTRRNAKWLTVEMMQDGHQVSLLSGELTVEQRASIIQRFRDGKEKVLIT
TNVCARGIDVKQVTIVVNFDLPVNQSEEPDYETYLHRIGRTGRFGKKGLAFNMIEVDKLPLLMKIQDPFN
SNIKQLDPEDMDEIEKIEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013932
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013932.1, NM_013932.2, NM_013932.3, NP_038960.1
RefSeq Size 1675 bp
RefSeq ORF 1455 bp
Locus ID 30959
UniProt ID Q9QY15
Cytogenetics 9 A4
MW 42.1 kDa
Summary ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.UniProtKB/Swiss-Prot Function
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"NM_013932" in other vectors (4)
SKU Description Size Price
MC205011 Ddx25 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25), (10ug) 10 ug
$457.00
MG205683 Ddx25 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$657.00
MR205683L3 Lenti ORF clone of Ddx25 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$757.00
MR205683L4 Lenti ORF clone of Ddx25 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$757.00

Plasmid Map for MR205683

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR205683
Circular map for MR205683

file-alt Citations

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