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Ddx25 (NM_013932) Mouse Untagged Clone

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SKU
MC205011
Ddx25 (untagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ddx25
Synonyms AW047046; GRTH
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC061130
AGGGGCGAGAGCGGAGACCGCAGCTATGGCGTCGTTACTTTGGGGAGGCGACGCCGGGGCGGCGGAGAGC GAGCGGCTGAACAGCCACTTTTCAAATCTCGTCCATCCCAGGAAGAACCTTCGTGGTATTAGGAGTACCA CAGTCCCAAACATAGACGGCTCTCTTAACACGGAAGATGATGATGATGATGAAGACGATGTAGTGGATTT GGCAGCCAATTCACTCTTAAACAAGCTAATCCGTCAGTCCTTAATAGAATCCAGCCACCGAGTGGAAGTC TTACAGAAGGATCCCAGCTCTCCACTCTACTCGGTGAAAACCTTTGAAGAGCTACGGCTAAAAGAAGAAT TACTAAAAGGGATTTATGCAATGGGATTTAACCGACCATCCAAAATCCAAGAGATGGCCCTCCCCATGAT GCTAGCACACCCTCCCCAGAACCTCATAGCACAGAGCCAGTCCGGCACAGGAAAAACTGCTGCCTTTGTC TTAGCAATGCTAAGCCGAGTGAATGCCTTGGAACTGTTTCCACAGTGCCTCTGCCTGGCTCCTACCTATG AACTGGCTCTGCAGACCGGTCGTGTGGTTGAAAGGATGGGCAAGTTCTGCGTGGATGTTGAAGTGATGTA TGCCATTCGAGGAAACCGAATTCCAAGGGGCACCGAAGTCACCAAACAGATTATTATTGGTACTCCTGGG ACTGTCCTAGATTGGTGTTTCAAGCGAAAACTGATTGATTTGACAAAGATCCGTGTGTTTGTCCTGGATG AAGCAGATGTGATGATTGACACCCAAGGATTCTCCGATCAGAGCATCCGTATCCAAAGAGCTTTACCCTC TGAATGCCAGATGCTCCTCTTTTCGGCAACCTTTGAGGACTCTGTGTGGCAGTTTGCTGAACGAATCATT CCTGACCCCAATGTTATCAAGTTGCGAAAAGAGGAACTGACCCTGAACAACATCCGGCAGTATTACGTGT TGTGTGAGAACAGGAAAGGCAAATACCAAGCCCTCTGTAACATCTACGGTGGCATCACCATTGGCCAAGC CATTATCTTCTGCCAGACACGTCGAAATGCCAAGTGGCTGACTGTGGAGATGATGCAGGATGGCCACCAG GTGTCCCTGCTAAGTGGGGAACTCACAGTGGAGCAGCGAGCTTCCATCATTCAGAGATTCCGAGATGGCA AAGAGAAGGTTCTCATAACGACCAATGTCTGTGCTCGAGGGATTGATGTGAAGCAGGTCACCATCGTCGT GAACTTTGACCTCCCTGTAAACCAATCAGAAGAGCCAGACTATGAGACCTACCTCCACCGTATAGGGCGG ACGGGACGCTTTGGGAAAAAAGGTCTCGCCTTCAACATGATTGAAGTGGATAAGCTGCCCCTGCTTATGA AAATACAAGACCACTTCAATAGCAATATTAAGCAGCTTGACCCAGAAGACATGGATGAGATTGAGAAGAT CGAATATTGAAGAGAGAGAGAGAAGAGAGGCCTTCGTTGTTTGTGGGCTAGTTTACGTGAGAATGTTTCA GCAGAGTTTGAAGATAATTTTCTATGTTGAGACTCTGTCAAGGTGAAACTGTCACAGATAAAAAATAAAT GCCATGACTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites SfiI-SfiI |
ACCN NM_013932
Insert Size 1110 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC061130, AAH61130
RefSeq Size 1652 bp
RefSeq ORF 1110 bp
Locus ID 30959
UniProt ID Q9QY15
Cytogenetics 9 A4
Summary ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013932" in other vectors (4)
SKU Description Size Price
MG205683 Ddx25 (tGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$657.00
MR205683 Ddx25 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$289.00 $457.00
MR205683L3 Lenti ORF clone of Ddx25 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$757.00
MR205683L4 Lenti ORF clone of Ddx25 (mGFP-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 25 (Ddx25) 10 ug
$757.00

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